165,688 results

1. eSrtA(4S-9) pET29b

   Plasmid

   #75146

   Purpose

   reprogrammed sortase A variant that recognizes alternative substrate LPXSG with high activity and specificity

   Depositor

   David Liu

   Article

   Dorr et al Proc Natl Acad Sci U S A. 2014 Sep 16;11

   Insert

   reprogrammed sortase A variant
   

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   N98D S102C A104V A118T F122A K134R F144L I182V E1…

   Promoter

   T7

   Available Since

   Aug. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDEST17-UbE2C

   Plasmid

   #15779

   Depositor

   Wade Harper

   Article

   Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

   Insert

   Ubiquitin conjugating enzyme UbE2C (UBE2C Human)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Sept. 28, 2007

   Availability

   Academic Institutions and Nonprofits only

3. pLV UBC hTMEM115-3C-3xHA-P2A-eGFP

   Plasmid

   #214996

   Purpose

   Stably express the GolgiTag (GolgiIP handle) in mammalian cells (human ORF). eGFP for cell sorting desired expression levels.

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   TMEM115 (TMEM115 Human)
   

   Use

   Lentiviral

   Tags

   3xHA

   Expression

   Mammalian

   Promoter

   UBC

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAC-BETA

   Plasmid

   #53272

   Purpose

   Contains crtE, crtB, crtI, and crtY carotenoid pathway genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces beta-carotene in Escherichia coli

   Depositor

   Francis X Cunningham Jr

   Article

   Cunningham FX et al Plant Cell. 1996 Sep;8(9):1613-26.

   Insert

   crtE, crtY, crtI, crtB
   

   Use

   Low copy number bacterial cloning vector

   Promoter

   endogenous promoters

   Available Since

   Jan. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-mCherry-Cre-blast

   Plasmid

   #179390

   Purpose

   Lentiviral expression of Cre recombinase and mCherry reporter from CMV promoter (separated by P2A peptide), and blasticidin resistance gene for selection in mammalian cells.

   Depositor

   Moshe Szyf

   Article

   Sapozhnikov et al Nat Commun. 2021 Sep 29;12(1):5711. doi:

   Insert

   mCherry (P2A) Cre recombinase
   

   Use

   Cre/Lox and Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPB-cT3G-cERP2-RUNX1

   Plasmid

   #192928

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of RUNX1

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   RUNX1 (RUNX1 Human)
   

   Expression

   Mammalian

   Promoter

   TET3G-miniCMV

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. N1-Tq-Ca-FLITS

   Plasmid

   #191454

   Purpose

   Genetically encoded calcium sensor (GECI) Tq-Ca-FLITS that reports with lifetime and intensity changes

   Depositor

   Dorus Gadella

   Article

   van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:

   Insert

   Tq-Ca-FLITS
   

   Expression

   Mammalian

   Available Since

   Nov. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCI-neo-hCG

   Plasmid

   #16574

   Depositor

   Bert Vogelstein

   Article

   Shih et al Nat Med. 2000 Jun . 6(6):711-4.

   Insert

   Beta-hCG (CGB3 Human)
   

   Expression

   Mammalian

   Available Since

   April 7, 2008

   Availability

   Academic Institutions and Nonprofits only

9. TRE-UBC-hCD47-T2A-GFP

   Plasmid

   #205458

   Purpose

   lentiviral plasmid for expression of human CD47

   Depositor

   Deepta Bhattacharya

   Article

   Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

   Insert

   hCD47 (CD47 Human)
   

   Expression

   Mammalian

   Promoter

   UBC

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV4-BlaM-Vpr

    Plasmid

    #21950

    Depositor

    Warner Greene

    Article

    Cavrois et al Nat Biotechnol. 2002 Nov . 20(11):1151-4

    Insert

    BlaM-Vpr (vpr Fusion of BlaM and HIV-1 Vpr)
    

    Expression

    Mammalian

    Available Since

    Oct. 1, 2009

    Availability

    Academic Institutions and Nonprofits only