164,829 results

1. pAAVS1-NC-CRISPRi (Gen3)

   Plasmid

   #73499

   Purpose

   Constitutive CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus

   Depositor

   Bruce Conklin

   Article

   Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

   Insert

   dCas9-KRAB
   

   Use

   CRISPR

   Tags

   HA, KRAB, and NLS

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically deactivated Cas9)

   Promoter

   CAG

   Available Since

   Dec. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHdzCas9-KRAB

   Plasmid

   #92341

   Purpose

   Expression dzCas9-KRAB in microalgae. CRISPR/Cas based plant genome editing and gene regulation, Hyg resistance

   Depositor

   I-Son Ng

   Article

   Kao et al Bioresour Technol. 2017 May 4. pii: S096

   Type

   Empty backbone

   Use

   CRISPR; Plant expression

   Tags

   KRAB

   Expression

   Plant

   Mutation

   Promoter

   CaMV35S

   Available Since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. TFORF0209

   Plasmid

   #141556

   Purpose

   Lentiviral vector for overexpressing the FOXP2 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   FOXP2 (FOXP2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAVS1-idCas9-vpr

   Plasmid

   #89985

   Purpose

   inducible CRISPR-ON system for controllable gene activation; this plasmid is used to insert dCas9-VPR casette in one allele of AAVS1 locus

   Depositor

   Jie Na

   Article

   Guo et al Protein Cell. 2017 Jan 23. doi: 10.1007/

   Insert

   dCas9-VPR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE-tight

   Available Since

   June 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-SUMO-hGSDMD

   Plasmid

   #111559

   Purpose

   Express human GSDMD in E.coli

   Depositor

   Hongbo Luo

   Article

   Kambara et al Cell Rep. 2018 Mar 13;22(11):2924-2936.

   Insert

   Gasdermin D (GSDMD Human)
   

   Use

   Tags

   His, SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pIRES2_EGFP_KCNQ1

   Plasmid

   #173161

   Purpose

   For mammalian expression of KCNQ1 and creating variants

   Depositor

   Al George

   Article

   Vanoye et al Circ Genom Precis Med. 2018 Nov;11(11):e

   Insert

   KCNQ1 (KCNQ1 Human)
   

   Use

   Tags

   IRES2-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBMN FKBP(DD)-YFP

   Plasmid

   #31763

   Depositor

   Thomas Wandless

   Article

   Banaszynski et al Cell. 2006 Sep 8;126(5):995-1004.

   Insert

   FKBP(DD)-YFP-HA (FKBP1A Human)
   

   Use

   Retroviral

   Tags

   HA and YFP

   Expression

   Mutation

   F36V, L106P on FKBP12

   Promoter

   LTR

   Available Since

   Oct. 3, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CAG-UnaG-P2A-FusionRed

   Plasmid

   #191109

   Purpose

   Mammalian cell line expression plasmids for production of UnaG and FusionRed proteins

   Depositor

   Kiryl Piatkevich

   Article

   Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

   Insert

   UnaG
   

   Use

   AAV

   Tags

   FusionRed

   Expression

   Mammalian

   Mutation

   See depositor comments below

   Promoter

   chicken β-actin promoter

   Available Since

   Nov. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pWR76

   Plasmid

   #202776

   Purpose

   episomal fluorescent reporter plasmid for S. stipitis

   Depositor

   J. Brian Robertson

   Article

   Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

   Inserts

   TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
   coHPH
   ACT1 terminator (S. stipitis)
   TDH3 promoter (S. stipitis)
   coCherry
   ADH2 terminator (S. stipitis)
   ARS from S. stipitis chromosome 1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBlu-CGT-1

    Plasmid

    #170158

    Purpose

    Expresses bacterial Cyclomaltodextrin Glucanotransferase from Paenibacillus sp. c36 in E.coli

    Depositor

    Sarah MacDonald

    Insert

    Cyclomaltodextrin Glucanotransferase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available Since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. TFORF3536

    Plasmid

    #145012

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    HOXD4 (HOXD4 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMBP-HRV3C-AcrIF9

    Plasmid

    #141442

    Purpose

    Expression of AcrIF9 in E. coli, tagged with an HRV3C-cleavage fusion to Maltose Binding Protein.

    Depositor

    Blake Wiedenheft

    Article

    Hirschi et al Nat Commun. 2020 Jun 1;11(1):2730. doi:

    Insert

    AcrIF9
    

    Use

    Tags

    HRV3C cleavage site and Maltose Binding Protein

    Expression

    Bacterial

    Mutation

    Promoter

    ptac

    Available Since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. BB2_AB

    Plasmid

    #98534

    Purpose

    empty BB2 for transcription unit cloning

    Depositor

    Michael Sauer, Brigitte Gasser, Diethard Mattanovich

    Article

    Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONR223_EGFR_p.G719D

    Plasmid

    #81708

    Purpose

    Gateway Donor vector containing EGFR, part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, Jesse Boehm, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    EGFR (EGFR Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    G719D

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET303_sumocodo_GFP_codoopti

    Plasmid

    #210393

    Purpose

    Express SUMO-GFP in E. coli

    Depositor

    Magnus Rueping

    Article

    Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

    Insert

    GFP
    

    Use

    Tags

    SUMO-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 13, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pEGFP-Aurora A

    Plasmid

    #198402

    Purpose

    Expression of EGFP-tagged Aurora A in mammalian cells

    Depositor

    Kenji Sugimoto

    Article

    Sugimoto et al Cell Struct Funct. 2002 Dec;27(6):457-67

    Insert

    Aurora A
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHR_PGK_SNIPR_CD8alpha SNIPR

    Plasmid

    #188357

    Purpose

    Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor

    Depositor

    Kole Roybal

    Article

    Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

    Insert

    PGK_antiCD19_CD8alpha-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMXs-Klf4

    Plasmid

    #13370

    Purpose

    Retroviral expression of mouse Klf4

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

    Insert

    Kruppel-like factor 4 (gut) (Klf4 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available Since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKLV2-EF1a-Cas9Bsd-W

    Plasmid

    #68343

    Purpose

    Lentiviral vector expressing Cas9 fused with the Blasticidin resistant gene at the C-terminus

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    EF1a-Cas9 cassette, WPRE
    

    Use

    CRISPR and Lentiviral

    Tags

    Cas9 fused with Flag-tag and a NLS at the N termi…

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. LV-TRE-VP64 human MyoD-T2A-dsRedExpress2

    Plasmid

    #60629

    Purpose

    Expresses VP64 human MyoD and dsRedExpress2 in response to doxycycline

    Depositor

    Charles Gersbach

    Article

    Kabadi et al ACS Synth Biol. 2014 Dec 10.

    Insert

    VP64 human MyoD (MYOD1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TRE

    Available Since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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