We narrowed to 59,379 results for: %s

1. pyc111s

   Plasmid

   #158781

   Purpose

   ribosome-tagged, Cre-dependent GCaMP for soma localization

   Depositor

   Jennifer Garrison, Zachary Knight

   Article

   Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

   Insert

   GCaMP6s-RPL10a
   

   Use

   AAV

   Available Since

   Sept. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-mGold-Calnexin-N-14

   Plasmid

   #158004

   Purpose

   Visualization of ER in mammalian cells using mGold

   Depositor

   Francois St-Pierre

   Article

   Lee et al Sci Adv. 2020 Oct 23;6(43). pii: 6/43/ea

   Insert

   Calnexin (CANX Human)
   

   Tags

   mGold

   Expression

   Mammalian

   Mutation

   I474N

   Promoter

   CMV

   Available Since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAVS1-NDi-CRISPRd (ABI-dCas9-mCherry)

   Plasmid

   #216531

   Purpose

   Targeting vector for CRISPRd generation

   Depositor

   Makiko Iwafuchi

   Article

   Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

   Insert

   ABI-dCas9-mCherry
   

   Use

   CRISPR

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   TRE3G

   Available Since

   Sept. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-U6sgp53-U6sgbbSapI-GFAPCre

   Plasmid

   #100275

   Purpose

   AAV-CRISPR mediated genome editing in glia / astrocytes

   Depositor

   Sidi Chen

   Article

   Chow et al Nat Neurosci. 2017 Aug 14. doi: 10.1038/

   Type

   Empty backbone

   Use

   AAV, CRISPR, Mouse Targeting, and Synthetic Biolo…

   Expression

   Mammalian

   Available Since

   March 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pQHA-USP11 CS puroR

   Plasmid

   #46750

   Purpose

   Retroviral vector that expresses catalytically inactive form of HA-tagged human USP11

   Depositor

   Goedele Maertens, Gordon Peters

   Article

   Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

   Insert

   USP11 (USP11 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   C318S--catalytically inactive

   Promoter

   CMV

   Available Since

   March 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCIG PIK3CA E545K

   Plasmid

   #73055

   Purpose

   Human PIK3CA E545K

   Depositor

   Joseph Gleeson

   Article

   Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

   Insert

   PIK3CA (PIK3CA Human)
   

   Expression

   Mammalian

   Mutation

   E545K

   Available Since

   March 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMXs-hs-LIN-41

   Plasmid

   #52716

   Purpose

   retroviral expression of human LIN-41 / TRIM71

   Depositor

   Shinya Yamanaka

   Article

   Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

   Insert

   LIN-41 (TRIM71 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   June 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. (564) pAAV Alb-AAT KRAB-SadCas9 U6-mPcsk9

   Plasmid

   #163025

   Purpose

   Expression of CRISPRi with gRNA against mouse Pcsk9

   Depositor

   Tonia Rex

   Article

   Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

   Insert

   Mammalian codon-optimized SadCas9 (Pcsk9 S. aureus)
   

   Use

   AAV

   Tags

   KRAB, SV40 pA, and myc NLS

   Expression

   Mammalian

   Promoter

   Alb-AAT

   Available Since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. bArr2-V5

   Plasmid

   #201494

   Purpose

   Expression of the beta-arrestin2 containing a N-terminal V5 tag.

   Depositor

   Patrick Giguère

   Article

   Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

   Insert

   bArr2-V5 (ARRB2 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Aug. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAGM22082_cRed

    Plasmid

    #117225

    Purpose

    pET-28b backbone plasmid, Level 2 Golden Gate expression plasmid.

    Depositor

    Sylvestre Marillonnet

    Article

    Pullmann et al Sci Rep. 2019 Jul 29;9(1):10932. doi: 10

    Insert

    Canthaxanthin Operon
    

    Tags

    34 amino acid N-terminal linker, Hexahistidine Ta…

    Expression

    Bacterial

    Mutation

    removed Type IIS restriction sites within the bac…

    Promoter

    T7 RNA Polymerase

    Available Since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. PB-LacI-eGFP

    Plasmid

    #213021

    Purpose

    piggyBac donor vector containing LacI-eGFP for random integration by PBase

    Depositor

    Luca Giorgetti

    Article

    Mach et al Nat Genet. 2022 Dec;54(12):1907-1918. do

    Insert

    LacI**-eGFP
    

    Use

    Mouse Targeting; Piggybase integration (transposo…

    Tags

    eGFP

    Expression

    Mammalian

    Available Since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJOG1336

    Plasmid

    #167303

    Purpose

    Level 0 module (3U+Ter) for further assemblies based on the Modular Cloning system. 3'UTR and flanking sequence of a Solanum lycopersicum Oleosin gene, arbitrarily called OLE2 (Sollyc03g112440)

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Insert

    3'UTR and Terminator SlOLE2
    

    Use

    Synthetic Biology; Moclo level 0 vector

    Available Since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJOG1334

    Plasmid

    #167302

    Purpose

    Level 0 module (Pro+5U) for further assemblies based on the Modular Cloning system. Promoter and 5'UTR of a Solanum lycopersicum Oleosin gene, arbitrarily called OLE2 (Sollyc03g112440)

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Insert

    Promoter SlOLE2
    

    Use

    Synthetic Biology; Moclo level 0 vector

    Available Since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTol2CG2-QUAS5x:GFPNLS-SV40pA

    Plasmid

    #155122

    Purpose

    Tol2 vector containing QUAS5x reporter element upstream of GFP-NLS. Includes cmlc2:mRFP-SV40pA transgenesis marker

    Depositor

    Bret Pearson

    Article

    Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

    Insert

    QUAS5x:GFPNLS
    

    Use

    Zebrafish expression

    Promoter

    QUAS5x-E1b

    Available Since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET19-MTdTwt

    Plasmid

    #207460

    Purpose

    Bacterial expression of Terminal deoxynucleotidyl transferase (TdT)

    Depositor

    Jay Keasling

    Article

    Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

    Insert

    MTdTwt (Dntt Mouse)
    

    Tags

    10xHis-MBP

    Expression

    Bacterial

    Available Since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pACEBac1-CoRESTfl

    Plasmid

    #109156

    Purpose

    Encodes human full-length CoREST with C-terminal 3xFLAG tag to be expressed in a baculovirus/insect cell expression system

    Depositor

    Monika Golas

    Article

    Inui et al Protein Sci. 2017 May;26(5):997-1011. do

    Insert

    human full-length CoREST, sequence-optimized for insect cells (RCOR1 Human)
    

    Tags

    3xFLAG

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    Jan. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-SpCas9-2A-GFP-noITR

    Plasmid

    #118415

    Purpose

    Cas9 GFP plasmid constitutively expressed from CAG promoter. AAV repeat region removed to increase expression

    Depositor

    Mark Denham

    Article

    Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

    Insert

    Cas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRR-ERbeta-5Z

    Plasmid

    #23062

    Depositor

    Charles Miller

    Article

    Miller CA et al Plasmid. 2010 Mar;63(2):73-8. doi: 10.10

    Insert

    ERbeta (ESR2 Human)
    

    Expression

    Yeast

    Available Since

    Jan. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

19. pGEL032

    Plasmid

    #137901

    Purpose

    STU2.0 LbCas12a for plant genome editing

    Depositor

    Yong Zhang

    Article

    Tang et al Plant Biotechnol J. 2019 Jul;17(7):1431-

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Feb. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. CDK1as_T2A_Zeo

    Plasmid

    #118596

    Purpose

    One of three plasmids required to create CDK1as human cells by single transfection (One shot system). CDK1as cDNA and Zeocin resistance cassette flanked by Sleeping Beauty transposon.

    Depositor

    William Earnshaw

    Article

    Saldivar et al Science. 2018 Aug 24;361(6404):806-810.

    Insert

    CDK1
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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