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pFX-β-tubulin-EGFP

Plasmid

#175827

Purpose

Express EGFP with microtubule-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

EGFP with microtuble-targeting sequence

Tags

human tubulin beta 4B class IVb;TUBB4B

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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Tet-O-FUW-FoxG1

Plasmid

#35547

Depositor

Marius Wernig

Article

Lujan et al Proc Natl Acad Sci U S A. 2012 Feb 14;10

Insert

FoxG1 (Foxg1 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

March 27, 2012

Availability

Academic Institutions and Nonprofits only

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pssAAV_Seq17_CMV_bglobin_3NLS_GFP_WPRE_polyA

Plasmid

#220221

Purpose

AAV-STARR-seq validation vector with GFP

Depositor

Simon Rumpel

Article

Chan et al Sci Rep. 2023 Apr 25;13(1):6745. doi: 10

Type

Empty backbone

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pET41a iGluu

Plasmid

#119834

Purpose

Production of recombinant iGluSnFR S72T ultrafast variant, a single-wavelength glutamate indicator, in E. coli

Depositor

Katalin Torok

Article

Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

Insert

iGluu

Tags

GST

Expression

Bacterial

Mutation

S72T

Promoter

T7

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-DEPTOR-WT

Plasmid

#159159

Purpose

Expresses DEPTOR in mammalian cells

Depositor

Michele Pagano

Article

Duan et al Mol Cell. 2011 Oct 21;44(2):317-24. doi:

Insert

DEPTOR (DEPTOR Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pROSA26-promoter

Plasmid

#21710

Depositor

Philippe Soriano

Article

Zambrowicz et al Proc Natl Acad Sci U S A. 1997 Apr 15. 9

Insert

ROSA26 promoter (Gt(ROSA)26Sor Mouse)

Expression

Mammalian

Available Since

July 28, 2009

Availability

Academic Institutions and Nonprofits only

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Shp2 biosensor

Plasmid

#134346

Purpose

FRET biosensor. To visualize Shp2 activity by FRET

Depositor

Yingxiao Wang

Article

Sun et al Nat Commun. 2017 Sep 7;8(1):477. doi: 10

Insert

Shp2 Biosensor

Expression

Mammalian

Available Since

Nov. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pSb_init containing anti SARS-CoV-2_RBD sybody Sb#45

Plasmid

#153526

Purpose

Expression of the anti SARS-CoV-2_RBD sybody Sb#45

Depositor

Markus Seeger

Article

Walter et al bioRxiv 2020.04.16.045419

Insert

Sb#45

Expression

Bacterial

Promoter

pBAD

Available Since

June 1, 2020

Availability

Academic Institutions and Nonprofits only

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pGex-Cav1

Plasmid

#33364

Depositor

Ambra Pozzi

Article

Borza et al J Biol Chem. 2010 Dec 17;285(51):40114-2

Insert

Cav1 (CAV1 Human)

Tags

GST

Expression

Bacterial

Available Since

Dec. 8, 2011

Availability

Academic Institutions and Nonprofits only
pCMV-Myc-FOXA1

Plasmid

#219394

Purpose

Express FOXA1 in mammalian cells

Depositor

Yoichi Furukawa

Article

Nakagawa et al Commun Biol. 2024 Apr 29;7(1):510. doi:

Insert

FOXA1 (FOXA1 Human)

Tags

N terminal Myc tag

Expression

Mammalian

Available Since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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pSL1180-HR-Aag2-loxP-PUb-RFP-loxP-3xFLAG-AGO1

Plasmid

#162164

Purpose

Selectable homology donor template for Aag2 cell AGO1

Depositor

Charles Rice

Article

Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

Inserts

RFP
3xFLAG

Use

CRISPR and Cre/Lox; Homology donor template

Tags

AGO1 homology and loxP

Expression

Insect

Promoter

Ae. aegypti PUb and endogenous AGO1

Available Since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-TFAP4

Plasmid

#110156

Purpose

Expression of human TFAP4 in mammalian cells

Depositor

Daniele Guardavaccaro

Article

D'Annibale et al J Biol Chem. 2014 Mar 14;289(11):7730-7.

Insert

TFAP4 (transcription factor AP-4) (TFAP4 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-Hsp1-Hsp2Cas9-KY-P2A-puro

Plasmid

#192133

Purpose

Expresses highly specific Hsp1-Hsp2Cas9, cloning backbone for sgRNA and puromycin resistance gene

Depositor

Yongming Wang

Article

Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

Insert

Hsp1-Hsp2Cas9-KY

Use

AAV

Expression

Mammalian

Mutation

Hsp1-Hsp2Cas9 (K390A, Y446A)

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG mEmerald-LAMP1

Plasmid

#168513

Purpose

Expression of rat LAMP1 fused with mEmerald to perform live tracking of lysosomes

Depositor

Franck Polleux

Article

Courchet et al Cell. 2013 Jun 20;153(7):1510-25. doi: 1

Insert

LAMP1 (Lamp1 Rat)

Tags

mEmerald

Expression

Mammalian

Promoter

CAG

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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(565) pAAV Alb-AAT KRAB-SadCas9 U6-gSTOP

Plasmid

#163030

Purpose

Expression of CRISPRi with gSTOP gRNA

Depositor

Tonia Rex

Article

Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

Insert

Mammalian codon-optimized SadCas9

Use

AAV

Tags

KRAB, SV40 pA, and myc NLS

Expression

Mammalian

Promoter

Alb-AAT

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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TaRGET-ABE-C3.1 ver4.1

Plasmid

#188251

Purpose

Hypercompact adenine base editors

Depositor

Yong-Sam Kim

Article

Kim et al Nat Chem Biol. 2022 Sep;18(9):1005-1013.

Insert

TaRGET-ABE3.1

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCSD_2xNLS-SpCas9WT-NLS-3xHA-NLS-SaCas9WT-2xNLS

Plasmid

#107320

Purpose

Expresses SpCas9 fused to SaCas9 in mammalian cells

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

SpCas9-SaCas9 fusion

Use

CRISPR

Tags

NLS

Expression

Mammalian

Mutation

SpCas9 is fused to SaCas9

Promoter

CMV IE94

Available Since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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attB-GFP

Plasmid

#65521

Purpose

Plasmid for the generation of GFP-knockin fusions via Bxb1-mediated recombination into MIN-tagged cell lines

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

attB-GFP

Use

Mouse Targeting; Bxb1

Expression

Mammalian

Available Since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pEntr1a-mcherry-FKBP-TIAM1(DHPH)

Plasmid

#176135

Purpose

Heterodimerization with FRB (rapamycin), Rac1 activation

Depositor

Dorus Gadella

Article

Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

Insert

TIAM1 (TIAM1 Human)

Use

Gateway Cloning

Mutation

See Depositor Comments Below

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pAV126

Plasmid

#63192

Purpose

pRS426 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

Depositor

Jef Boeke

Article

Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial and Yeast

Available Since

Aug. 30, 2017

Availability

Academic Institutions and Nonprofits only

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