We narrowed to 21,629 results for: ESC;

1. pFX-Lifeact-EGFP

   Plasmid

   #174477

   Purpose

   Express EGFP with actin-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   EGFP with actin-targeting sequence
   

   Use

   Tags

   Lifeact (N-terminal 17 amino acids from S. Cerevi…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFX-SECFP-Sec61β

   Plasmid

   #174465

   Purpose

   Express SECFP with endoplasmic reticulum-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   SECFP with endoplasmic reticulum-targeting sequence
   

   Use

   Tags

   Sec61 subunit beta

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFX-iRFP-KRasCT

   Plasmid

   #175836

   Purpose

   Express iRFP with plasma membrane-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   iRFP with plasma membrane-targeting sequence
   

   Use

   Tags

   C-terminal hypervariable region of K-Ras 4B

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFX-Lifeact-SECFP

   Plasmid

   #174476

   Purpose

   Express SECFP with actin-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   SECFP with actin-targeting sequence
   

   Use

   Tags

   Lifeact (N-terminal 17 amino acids from S. Cerevi…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pFX-Lifeact-Sirius

   Plasmid

   #174475

   Purpose

   Express Sirius with actin-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   Sirius with actin-targeting sequence
   

   Use

   Tags

   Lifeact (N-terminal 17 amino acids from S. Cerevi…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pFX-Tom20-Sirius

   Plasmid

   #174184

   Purpose

   Express Sirius with mitochondria-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   Sirius with mitchondria-targeting sequence
   

   Use

   Tags

   the mitochondria-targeting sequence of TOM20

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFX-Sirius-Sec61β

   Plasmid

   #174464

   Purpose

   Express Sirius with endoplasmic reticulum-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   Sirius with endoplasmic reticulum-targeting sequence
   

   Use

   Tags

   Sec61 subunit beta

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFX-GalT-Sirius

   Plasmid

   #174469

   Purpose

   Express Sirius with golgi apparatus-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   Sirius with golgi apparatus-targeting sequence
   

   Use

   Tags

   β1,4-galactose transferase

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pFX-iRFP-Rab7

   Plasmid

   #174459

   Purpose

   Express iRFP with late endosome-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   iRFP with late endosome-targeting sequence
   

   Use

   Tags

   small GTPase (Rab7)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBS-KS-attB1-2-PT-SA-SD-0-mTagBFP2(11x7)

    Plasmid

    #172064

    Purpose

    Protein trap plasmid for inserting mTagBFP2(11x7) into a phase 0 coding intron

    Depositor

    Daichi Kamiyama

    Article

    Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

    Insert

    mTagBFP2(11x7)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 20, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pBN216

    Plasmid

    #167963

    Purpose

    express E. coli E69 (O9a:K30) Wzc with Y713E, Y715E, Y717E, Y718E

    Depositor

    James Naismith

    Article

    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

    Insert

    E. coli E69 (O9a:K30) Wzc with Y713E, Y715E, Y717E, Y718E
    

    Use

    Tags

    hexa-histidine tag

    Expression

    Bacterial

    Mutation

    Y713E, Y715E, Y717E, Y718E

    Promoter

    araBAD

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBN217

    Plasmid

    #167964

    Purpose

    express E. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E, Y718E

    Depositor

    James Naismith

    Article

    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

    Insert

    E. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E, Y718E
    

    Use

    Tags

    hexa-histidine tag

    Expression

    Bacterial

    Mutation

    Y708E, Y713E, Y715E, Y717E, Y718E

    Promoter

    araBAD

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBN262

    Plasmid

    #167967

    Purpose

    express E. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E

    Depositor

    James Naismith

    Article

    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

    Insert

    E. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E
    

    Use

    Tags

    hexa-histidine tag

    Expression

    Bacterial

    Mutation

    Y708E, Y713E, Y715E, Y717E

    Promoter

    araBAD

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBRC901

    Plasmid

    #167948

    Purpose

    express wild-type bacterial tyrosine kinase Wzc from E. coli E69 (O9a:K30)

    Depositor

    James Naismith

    Article

    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

    Insert

    wild-type bacterial tyrosine kinase Wzc from E. coli E69 (O9a:K30)
    

    Use

    Tags

    hexa-histidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBN202

    Plasmid

    #167950

    Purpose

    express E. coli E69 (O9a:K30) Wzc_K540M with Y713E, Y715E, Y717E, Y718E mutations

    Depositor

    James Naismith

    Article

    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

    Insert

    E. coli E69 (O9a:K30) Wzc_K540M with Y713E, Y715E, Y717E, Y718E mutations
    

    Use

    Tags

    hexa-histidine tag

    Expression

    Bacterial

    Mutation

    K540M, Y713E, Y715E, Y717E, Y718E

    Promoter

    araBAD

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJS697

    Plasmid

    #168778

    Purpose

    Yeast surface display plasmid backbone that can be combined with pJS699 through homologous recombination

    Depositor

    Timothy Whitehead

    Article

    Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

    Type

    Empty backbone

    Use

    Tags

    Cmyc and HA

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. 4LNDD-pCDNA3.1(+)

    Plasmid

    #172227

    Purpose

    Expresses human ADAR2 Deaminase Domain (DD) fused with 4 repeats of lambda N peptide (4LN) in mammalian cells

    Depositor

    Joshua Rosenthal

    Article

    Montiel-Gonzalez et al Nucleic Acids Res. 2016 Dec 1;44(21):e15

    Insert

    Lambda N peptide - human ADAR2 Deaminase Domain
    

    Use

    Tags

    6xHis and Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pKP1693

    Plasmid

    #167595

    Purpose

    Express FepA with His6 tag between residues 393 and 394 of the mature amino acid sequence . Please note that mutation numbers are not based on start codon of insert.

    Depositor

    Kathleen Postle

    Article

    Gresock et al J Bacteriol. 2017 Apr 25;199(10). pii: J

    Insert

    FepA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    His6 tag inserted after residue 393 in the mature…

    Promoter

    Available since

    July 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pKB238

    Plasmid

    #170033

    Purpose

    Encodes LacI-controlled expression of surface-displayed NPY and PvirK-mNeonGreen reporter

    Depositor

    Jeffrey Tabor

    Article

    Brink et al bioRxiv

    Inserts

    mNeonGreen
    Neuropeptide Y (NPY Synthetic, Escherichia coli, Human)
    

    Use

    Synthetic Biology

    Tags

    Lpp, OmpA, and Tether

    Expression

    Bacterial

    Mutation

    Promoter

    Ptac and PvirK

    Available since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pKB235

    Plasmid

    #170024

    Purpose

    Encodes LacI-controlled expression of surface-displayed CCL20 and PvirK-mNeonGreen reporter

    Depositor

    Jeffrey Tabor

    Article

    Brink et al bioRxiv

    Inserts

    mNeonGreen
    CCL20 (CCL20 Synthetic, Escherichia coli, Human)
    

    Use

    Synthetic Biology

    Tags

    Lpp, OmpA, and Tether

    Expression

    Bacterial

    Mutation

    Promoter

    Ptac and PvirK

    Available since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge