We narrowed to 15,677 results for: Nes
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Plasmid#184102Purposecyclofen-inducible fish Hoxa13b activation via zebrafish transient transgenesis or mRNA synthesisDepositorInsert5myc-ZfHoxa13b-ERT2-P2A-mCherry
UseZebrafish transgenesis (blue eyes) + in vitro tra…Tags5xMycMutationERT2: G400V, M543A, L544A, deleted 1-281;PromoterZf-Ubi+SP6Available SinceJuly 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVZ_PcoaT_SQE1_LUP1
Plasmid#103857PurposeHeterologous, cobalt-inducible expression of SQE1 and LUP1 from A. thaliana in Synechocystis sp. PCC6803DepositorInsertlupeol synthase 1 (LUP1 Mustard Weed)
UseSynthetic BiologyExpressionBacterialMutationinserted genes are codon optimized. 2nd amino aci…PromoterPcoaT (from Synechocystis sp. PCC 6803)Available SinceJuly 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag NME7
Plasmid#20509DepositorAvailable SinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
Alt AlphaPS3
Plasmid#14070DepositorInsertDrosphila Alt Alpha PS3 Integrin (scb Fly)
ExpressionBacterialAvailable SinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSL012_AAVS1-puro-9xTetO-pEF-H2B-mCitrine-5kb,lambda-pRSV-H2B-mCherry
Plasmid#179429PurposeDual fluorescent reporter construct with 5kb lambda spacer, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locusDepositorInsertTRE-cit-5kb-mch
ExpressionMammalianPromoterpEF alpha, pRSVAvailable SinceFeb. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
GP804
Plasmid#164548PurposeExpresses Smo-FLAG K543, 565, 568, 569, 575, 579, 628, 671, 676, 677, 678, 681, 682, 683, 684, 717RDepositorInsertSmoothened K543, 565, 568, 569, 575, 579, 628, 671, 676, 677, 678, 681, 682, 683, 684, 717R (Smo Mouse)
UseLentiviralTagsFLAGMutationAll sixteen Lysines in cytoplasmic C-terminal tai…PromoterGgCryD1Available SinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-SpyTag-Flag-dNS-SRRM1-V5-mCherry
Plasmid#235096PurposeSRRM1 lacking Nuclear Speckle domain (dNS-SRRM1) mCherry fusion protein without MCP domainDepositorInsertpCAG-SpyTag-Flag-dNS-SRRM1-V5-mCherry (SRRM1 Human)
ExpressionMammalianMutationThe SRRM1 entry clone from DNASU was modified usi…PromoterCAGAvailable SinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-TSC2(AA,3Q)-NLS
Plasmid#192445PurposeEncodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A, K1614Q, K1615Q, R1616Q mutations to disrupt Akt phosphorylation and GAP activity; targeted to the nucleus.DepositorInsertmCherry-TSC2(S939A,T1439A,K1614Q,K1615Q,R1616Q )-NLS (TSC2 Human)
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationTSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …PromoterCMVAvailable SinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
R989-X31-566: His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61L
Plasmid#159551PurposeE. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61LDepositorInsertHis6-MBP-tev-G-Hs.KRAS4b(1-188) Q61L (KRAS Human)
TagsHis6-MBP-tevExpressionBacterialMutationQ61LAvailable SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
R989-X13-566: His6-MBP-tev-G-Hs.KRAS4b(1-169) Q61R
Plasmid#159545PurposeE. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-169) Q61RDepositorInsertHis6-MBP-tev-G-Hs.KRAS4b(1-169) Q61R (KRAS Human)
TagsHis6-MBP-tevExpressionBacterialMutationQ61RAvailable SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
R989-X12-566: His6-MBP-tev-G-Hs.KRAS4b(1-169) Q61L
Plasmid#159544PurposeE. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-169) Q61LDepositorInsertHis6-MBP-tev-G-Hs.KRAS4b(1-169) Q61L (KRAS Human)
TagsHis6-MBP-tevExpressionBacterialMutationQ61LAvailable SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
R989-X27-636: His6-MBP-tev-G-Hs.KRAS4b(1-188) G12C
Plasmid#159554PurposeBaculovirus protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) G12CDepositorInsertHis6-MBP-tev-G-Hs.KRAS4b(1-188) G12C (KRAS Human)
TagsHis6-MBP-tevExpressionInsectMutationG12CAvailable SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
R989-X30-566: His6-MBP-tev-G-Hs.KRAS4b(1-188) G13D
Plasmid#159550PurposeE. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) G13DDepositorInsertHis6-MBP-tev-G-Hs.KRAS4b(1-188) G13D (KRAS Human)
TagsHis6-MBP-tevExpressionBacterialMutationG13DAvailable SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
R989-X29-636: His6-MBP-tev-G-Hs.KRAS4b(1-188) G12V
Plasmid#159556PurposeBaculovirus protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) G12VDepositorInsertHis6-MBP-tev-G-Hs.KRAS4b(1-188) G12V (KRAS Human)
TagsHis6-MBP-tevExpressionInsectMutationG12VAvailable SinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
R989-X31-636: His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61L
Plasmid#159558PurposeBaculovirus protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61LDepositorInsertHis6-MBP-tev-G-Hs.KRAS4b(1-188) Q61L (KRAS Human)
TagsHis6-MBP-tevExpressionInsectMutationQ61LAvailable SinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 extracellular-cysteineless (NT864)
Plasmid#49069PurposeExpresses human NKCC1 mutant lacking extracellular cysteine residues and with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
Tags3x Flag and mVenusExpressionMammalianMutationC563S,C568S,C577S,C582S in hNKCC1 in NT17PromoterCMVAvailable SinceNov. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
PGL4.11-COL1A1double mutants
Plasmid#230918PurposeDouble mutation of TEAD and SMAD binding elements in COL1A1 promoter; check the promoter activity.DepositorInsertHuman CLO1A1 promoter including SMAD and TEAD mutant elements (COL1A1 Human)
UseLuciferaseTagsLuciferase-luc2pExpressionBacterial and MammalianMutationCOL1A1 promoter with mutant SMAD and TEAD binding…PromoterCOL1A1 with mutant SMAD and TEAD elementsAvailable SinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMH004_phiC31_neo-HS4-5xTetO-pEF-H2B-mCitrine-HS4-pRSV-H2B-mCherry
Plasmid#179432PurposeDual fluorescent reporter construct with double HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, phiC31 attB for integrationDepositorInsertHS4-TRE-cit-HS4-mch (DH)
ExpressionMammalianPromoterpEF alpha, pRSVAvailable SinceMay 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMH003_phiC31_neo-5xTetO-pEF-H2B-mCitrine-coreHS4-pRSV-H2B-mCherry
Plasmid#179431PurposeDual fluorescent reporter construct with single core HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, phiC31 attB for integrationDepositorInsertTRE-cit-coreHS4-mch (SC)
ExpressionMammalianPromoterpEF alpha, pRSVAvailable SinceMay 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
GP805
Plasmid#164545PurposeExpresses Smo-FLAG K348, 360, 434, 444, 448, 543, 565, 568, 569, 575, 579, 628, 671, 676, 677, 678, 681, 682, 683, 684, 717RDepositorInsertSmoothened K348, 360, 434, 444, 448, 543, 565, 568, 569, 575, 579, 628, 671, 676, 677, 678, 681, 682, 683, 684, 717R (Smo Mouse)
UseLentiviralTagsFLAGMutationAll 21 cytoplamic Lysines of Smoothened changed t…PromoterGgCryD1Available SinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only