We narrowed to 11,455 results for: Vars;
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Plasmid#148883PurposeInsect Expression of DmHelz_1213-2103DepositorInsertDmHelz_1213-2103 (CG9425 Fly)
ExpressionInsectMutationtwo silent an two non silent G1374S, R2076G mutat…Available SinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-myc-TRBP-dsRBD2
Plasmid#180602PurposeExpressing various parts of TRBPDepositorAvailable SinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-myc-TRBP-dsRBD1
Plasmid#180601PurposeExpressing various parts of TRBPDepositorAvailable SinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-TRBP-L2+D2+L3-myc/His
Plasmid#180600PurposeExpressing various parts of TRBPDepositorAvailable SinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-F594V-siRNAres_F
Plasmid#146331PurposeInsect Expression of DmAGO1-F594VDepositorInsertDmAGO1-F594V (AGO1 Fly)
ExpressionInsectMutationvariation with 2 non silent mutations compared to…Available SinceFeb. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-F2V2_A
Plasmid#145896PurposeInsect Expression of DmAGO1-F2V2DepositorInsertDmAGO1-F2V2 (AGO1 Fly)
ExpressionInsectMutationvariation with 2 non silent mutations compared to…Available SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-F594V_A
Plasmid#145897PurposeInsect Expression of DmAGO1-F594VDepositorInsertDmAGO1-F594V (AGO1 Fly)
ExpressionInsectMutationvariation with 2 non silent mutations compared to…Available SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-siRNAres_A
Plasmid#145899PurposeInsect Expression of DmAGO1-siRNAresDepositorInsertDmAGO1-siRNAres (AGO1 Fly)
ExpressionInsectMutationvariation with 2 non silent mutations compared to…Available SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
bdSUMO-5.3-GFP-HiBit
Plasmid#171014PurposeFor bacterial expression of bdSUMO tagged muGFP with 5.3 peptide and NanoLuc complementation peptide variant #86DepositorInsert14x-His-bdSUMO-5.3-muGFP-HiBiT
UseLuciferaseExpressionBacterialPromoterT7Available SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-acVHH (Y34W)-PB
Plasmid#171072PurposeA COSMO mutant (Y34W) responds to caffeine to induce dimerization of a phospholipid-binding polybasic domain with subsequent plasma membrane translocation.DepositorInsertanti-caffeine nanobody variant
Tagsmodified STIM1–PB tagExpressionMammalianMutationY34WPromoterCMVAvailable SinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-acVHH (Y34F)-PB
Plasmid#171071PurposeA COSMO mutant (Y34F) responds to caffeine to induce dimerization of a phospholipid-binding polybasic domain with subsequent plasma membrane translocation.DepositorInsertanti-caffeine nanobody variant
Tagsmodified STIM1–PB tagExpressionMammalianMutationY34FPromoterCMVAvailable SinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP–acVHH (Y104F)–PB
Plasmid#169683PurposeA COSMO mutant (Y104F) responds to caffeine to induce dimerization of a phospholipid-binding polybasic domain with subsequent plasma membrane translocation.DepositorInsertanti-caffeine nanobody variant
Tagsmodified STIM1–PB tagExpressionMammalianMutationY104FPromoterCMVAvailable SinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-F629V_A
Plasmid#145898PurposeInsect Expression of DmAGO1-F629VDepositorInsertDmAGO1-F629V (AGO1 Fly)
ExpressionInsectMutationV315A, F629V, H913R - variation with 2 non silent…Available SinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGG422_UV5
Plasmid#165607PurposeVector for expression of the SpCas9 KG variant with sgRNA in E. coli: KG(SpCas9, D1332K/R1333G) and UV5-sgRNA (hEGFP spacer)DepositorInsertlacUV5 driving Streptococcus pyogenes Cas9 KG(D1332K/R1333G) and hEGFP-sgRNA
UseCRISPR and Synthetic BiologyExpressionBacterialMutationD1332K and R1333G mutations in SpCas9PromoterlacUV5 driving Cas9 KG and UV5 driving sgRNAAvailable SinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET51b-His-TEV-SNAP-tag_dead_mt
Plasmid#167270PurposeOverexpression of the dead variant (C145A) of SNAP-tag in E. coliDepositorInsertSNAP-tag
TagsHis x10ExpressionBacterialMutationC145APromoterT7Available SinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET51b-His-TEV-SNAP-tag_fast_dead_mt
Plasmid#167272PurposeOverexpression of the fast (E30R) dead variant (C145A) of SNAP-tag in E. coliDepositorInsertSNAPf-tag
TagsHis x10ExpressionBacterialMutationE30R-C145APromoterT7Available SinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-GFP-EBA175-Flag(P1424G)
Plasmid#155128PurposeExpresses N-terminally GFP-tagged EBA175 P1424G variant (residues 1284-1462) from pcDNA3.1DepositorInsertPfEBA175
TagsFlag, GFP, and signal peptide (residues 1 to 32) …ExpressionMammalianMutationP1424G; insert codes for EBA175 residues 1284 to …PromoterCMVAvailable SinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HA-PfROM4 (H578A)
Plasmid#154858PurposeExpresses N-terminally HA-tagged PfROM4 variant H578A (codon optimized for mammalian expression) from pcDNA3.1DepositorInsertPfROM4 (PF3D7_0506900 Synthetic)
Tagstriple HAExpressionMammalianMutationHistidine 578 changed to AlaninePromoterCMVAvailable SinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HA-PfROM4 (G470A/G471N)
Plasmid#154859PurposeExpresses N-terminally HA-tagged PfROM4 variant G470A/G471N (codon optimized for mammalian expression) from pcDNA3.1DepositorInsertPfROM4 (PF3D7_0506900 Synthetic)
Tagstriple HAExpressionMammalianMutationGlycine 470 changed to Alanine and Glycine 471 ch…PromoterCMVAvailable SinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HA-PfROM1 (A144G/N145G)
Plasmid#154873PurposeExpresses N-terminally HA-tagged PfROM1 variant A144G/N145G (codon optimized for mammalian expression) from pcDNA3.1DepositorInsertPfROM1 (PF3D7_0506900 Synthetic)
Tagstriple HAExpressionMammalianMutationAlanine 144 changed to Glycine and Asparagine 145…PromoterCMVAvailable SinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only