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We narrowed to 60,317 results for: cloning

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Showing: 10461 - 10480 of 60317 results

  1. pDest-527

    Plasmid
    #11518
    Purpose
    Gateway destination vector for bacterial expression; His tag
    Depositor
    Dominic Esposito
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available Since
    May 24, 2006
    Availability
    Academic Institutions and Nonprofits only
    11518-plasmid-map-sequence-id-181065

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  2. pLKO.1 neo

    Plasmid
    #13425
    Purpose
    3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses neomycin for selection.
    Depositor
    Sheila Stewart
    Insert
    non-hairpin 18bp
    Use
    Lentiviral and RNAi
    Expression
    Mammalian
    Promoter
    U6 promoter
    Available Since
    Dec. 22, 2006
    Availability
    Academic Institutions and Nonprofits only
    13425-plasmid-map-sequence-id-360003

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  3. ER-P-TAG (pGS594)

    Plasmid
    #160039
    Purpose
    pLV-A4-UCOE-EF1a-CP450TMD-APEX2-3xHA-IRES-BLAS-2A-SNAP-WPRE-A2
    Depositor
    David Sabatini
    Insert
    CP450TMD-APEX2-3xHA
    Use
    Lentiviral
    Promoter
    EF1
    Available Since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    160039-plasmid-map-sequence-id-327043

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  4. BLMA-c800

    Plasmid
    #53631
    Purpose
    bacterial expression plasmid
    Depositor
    Nicola Burgess-Brown
    Insert
    BLMA
    Tags
    His-Tev
    Expression
    Bacterial
    Mutation
    Bacterial expression for structure determination;…
    Promoter
    T7
    Available Since
    June 17, 2014
    Availability
    Academic Institutions and Nonprofits only

  5. MZB211

    Plasmid
    #228494
    Purpose
    10xHis-SUMO (Smt3) tagged Ssod7-fused Pfu Polymerase with fidelity enhancing mutation (E. coli based protein expression)
    Depositor
    Richard Baker
    Insert
    pol
    Tags
    10xHis-SUMO(Smt3)
    Expression
    Bacterial
    Mutation
    A408S
    Promoter
    T7 Promoter
    Available Since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    228494-plasmid-map-sequence-id-455979

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  6. pX601 miniCMV-SaCas9-SpA-sgRNA scaffold

    Plasmid
    #107055
    Purpose
    Backbone vector for cloning in target sgRNA for use with SaCas9 (SauCas9)
    Depositor
    Alex Hewitt
    Type
    Empty backbone
    Use
    AAV and CRISPR
    Expression
    Mammalian
    Available Since
    April 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    107055-plasmid-map-sequence-id-208591

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  7. pCW57.1_TStrp_HA_Nt

    Plasmid
    #194065
    Purpose
    3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blasti
    Depositor
    RESOLUTE Consortium, Giulio Superti-Furga
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    HA-Twin_Strep
    Expression
    Mammalian
    Available Since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    194065-plasmid-map-sequence-id-380704

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  8. pLenti-hSyn1-mRuby2-T2A-GCaMP6f

    Plasmid
    #197595
    Purpose
    Lentiviral transfer vector for the neuronal expression of mRuby2-T2A-GCaMP6f (fluorescent reporter for Calcium imaging)
    Depositor
    Patrik Verstreken
    Insert
    mRuby2-T2A-GCaMP6f
    Use
    Lentiviral
    Promoter
    hSynI
    Available Since
    April 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    197595-plasmid-map-sequence-id-389365

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  10. pDual_dsCas9_Blast

    Plasmid
    #214681
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Blasticidin resistance casette with two empty sgRNA cloning sites
    Depositor
    Kevin Janes
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Available Since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214681-plasmid-map-sequence-id-428811

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  11. pHN922

    Plasmid
    #178975
    Purpose
    Expression of GST-His8-GFP-nanobody
    Depositor
    Kazuhiro Abe
    Insert
    GST-His8-GFP-nanobody
    Use
    Affinity Reagent/ Antibody
    Tags
    GST, His8
    Expression
    Bacterial
    Promoter
    tac
    Available Since
    Feb. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    178975-plasmid-map-sequence-id-354968

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  12. pDisplay-GRAB_HTR6-1.0

    Plasmid
    #180005
    Purpose
    Expresses the 5-HT sensor GRAB_HTR6-1.0 in mammalian cells
    Depositor
    Yulong Li
    Insert
    5-HT sensor GRAB_HTR6-1.0
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    180005-plasmid-map-sequence-id-358496

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  13. SARS_pNIC-CTB10H

    Plasmid
    #153056
    Purpose
    Expression construct for producing Avi tagged Seryl-tRNA synthetase
    Depositor
    Susanne Gräslund
    Insert
    SARS (SARS1 Human)
    Tags
    Avi
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    June 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    153056-plasmid-map-sequence-id-304476

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  14. BAZ2AA-c002

    Plasmid
    #53623
    Purpose
    bacterial expression plasmid
    Depositor
    Nicola Burgess-Brown
    Insert
    BAZ2AA
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    Bacterial expression for structure determination;…
    Promoter
    T7
    Available Since
    June 3, 2014
    Availability
    Academic Institutions and Nonprofits only

  15. AAV-syn-axon-jGCaMP8s-WPRE

    Plasmid
    #186043
    Purpose
    jGCaMP8s expression in axons under the control of the synapsin promoter
    Depositor
    Loren Looger
    Insert
    axon-jGCaMP8s
    Use
    AAV
    Tags
    GAP43-6xHis
    Expression
    Mammalian
    Promoter
    synapsin
    Available Since
    Aug. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    186043-plasmid-map-sequence-id-370174

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  16. AAV-syn-axon-jGCaMP8f-WPRE

    Plasmid
    #186041
    Purpose
    jGCaMP8f expression in axons under the control of the synapsin promoter
    Depositor
    Loren Looger
    Insert
    axon-jGCaMP8f
    Use
    AAV
    Tags
    GAP43-6xHis
    Expression
    Mammalian
    Promoter
    synapsin
    Available Since
    Aug. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    186041-plasmid-map-sequence-id-370171

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  17. p3E_he1a:mCherry

    Plasmid
    #113881
    Purpose
    p3E entry vector for Three Multisite Gateway
    Depositor
    Marnie Halpern
    Insert
    he1a (he1.1 Zebrafish)
    Use
    Zebrafish danio expression
    Tags
    mCherry
    Available Since
    Sept. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    113881-plasmid-map-sequence-id-223633

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  18. pCW57.1_V5_FLAG_Ct

    Plasmid
    #194075
    Purpose
    3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blasti
    Depositor
    RESOLUTE Consortium, Giulio Superti-Furga
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    FLAG-V5
    Expression
    Mammalian
    Available Since
    Jan. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    194075-plasmid-map-sequence-id-381944

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  19. CROP-seq-opti-dsRed

    Plasmid
    #201999
    Purpose
    Based on CROP-seq-opti (addgene #106280), we replaced the PURO resistance gene with a dsRed.
    Depositor
    Daniel Geschwind
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    dsRed
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    201999-plasmid-map-sequence-id-398468

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  20. mKikGR-N1

    Plasmid
    #54756
    Purpose
    Localization: N1 Cloning Vector, Excitation: 507 / 583, Emission: 517 / 593
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    mKikGR
    Expression
    Mammalian
    Available Since
    June 12, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54756-plasmid-map-sequence-id-492633

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Showing: 10461 - 10480 of 60317 results