We narrowed to 24,060 results for: crispr

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pxpr_050-wt-ltr

Plasmid

#182646

Purpose

lentiviral expression of gRNA scaffold, contains intact LTR

Depositor

Jakob Reiser

Article

Iaffaldano et al Front Genome Ed. 2023 Jul 13;5:1218328.

Insert

None

Use

Lentiviral

Expression

Mammalian

Available Since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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pJET1.2-STOP-dsRed

Plasmid

#60944

Purpose

Drosophila, Splicing acceptor, STOP, dsRED, RFP, SV40 terminator, flanked attP. section marker, assemble with homology arms to make Donor plasmid for CRISPR.

Depositor

Frank Schnorrer

Article

Zhang et al G3 (Bethesda). 2014 Oct 15. pii: g3.114.

Insert

dsRED

Use

Unspecified

Promoter

3XP3

Available Since

Nov. 19, 2014

Availability

Academic Institutions and Nonprofits only

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p426*-SaSgH

Plasmid

#136994

Purpose

Helper plasmid for SaCas9 gRNA cloning and expression

Depositor

Huimin Zhao

Article

Lian et al Nat Commun. 2019 Dec 19;10(1):5794. doi:

Type

Empty backbone

Expression

Yeast

Promoter

SNR52

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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pRN1120

Plasmid

#101750

Purpose

Multi-copy (2 micron) yeast plasmid containing a functional NatMX marker cassette conferring resistance against nourseothricin. Recipient plasmid for sgRNA or crRNA expression cassettes.

Depositor

Rene Verwaal

Article

Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278

Type

Empty backbone

Expression

Yeast

Available Since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pJH375

Plasmid

#86841

Purpose

mamalian expression of anti-CRISPR protein AcrIIA3

Depositor

Joseph Bondy-Denomy

Article

Rauch et al Cell. 2016 Dec 22. pii: S0092-8674(16)31

Insert

acrIIA3

Expression

Mammalian

Promoter

CMV

Available Since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

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p426*-SpSgH

Plasmid

#136993

Purpose

Helper plasmid for SpCas9 gRNA cloning and expression

Depositor

Huimin Zhao

Article

Lian et al Nat Commun. 2019 Dec 19;10(1):5794. doi:

Type

Empty backbone

Expression

Yeast

Promoter

SNR52

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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PX853

Plasmid

#62885

Purpose

N-term SpCas9 piece of inducible split-5 (Cas9(N)-FRB-NES split-5).

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

Insert

SpCas9 (aa 2-573)

Use

CRISPR

Tags

FRB and NES PTK2

Expression

Mammalian

Promoter

CBh

Available Since

March 5, 2015

Availability

Academic Institutions and Nonprofits only

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bRA90

Plasmid

#100951

Purpose

Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. LEU2 marker

Depositor

James Haber

Article

Anand et al Nature. 2017 Apr 20;544(7650):377-380. d

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pRVV661

Plasmid

#108282

Purpose

Donor vector for creation of PhiC31 attP-flanked platforms for cassette exchange using CRISPR/Cas9

Depositor

Richard Mann

Article

Voutev et al G3 (Bethesda). 2018 Mar 9. pii: g3.118.2

Insert

PhiC31 attP sites

Use

CRISPR

Available Since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

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bRA77

Plasmid

#100953

Purpose

GAL1 driven Cas9. gRNA can be cloned into BplI sites.

Depositor

James Haber

Article

Anand et al Nature. 2017 Apr 20;544(7650):377-380. d

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

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hfDECOR-F148A

Plasmid

#219546

Purpose

Expression of CMV-TadA8e-F148A-NLS-dCas13d-NLS in mammalian cells (hfDECOR-F148A expression)

Depositor

Weixin Tang

Article

Yan et al Nat Chem Biol. 2024 Jul 5. doi: 10.1038/

Insert

hfDECOR-F148A

Use

CRISPR

Expression

Mammalian

Mutation

Changed TadA8e Phenylalanine 148 to Alanine

Promoter

CMV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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hfDECOR-V106G

Plasmid

#219547

Purpose

Expression of CMV-TadA8e-V106G-NLS-dCas13d-NLS in mammalian cells (hfDECOR-V106G expression)

Depositor

Weixin Tang

Article

Yan et al Nat Chem Biol. 2024 Jul 5. doi: 10.1038/

Insert

hfDECOR-V106G

Use

CRISPR

Expression

Mammalian

Mutation

Changed TadA8e Valine 106 to Glycine

Promoter

CMV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pRVV669

Plasmid

#108283

Purpose

Donor vector for creation of PhiC31 attP-flanked platforms for cassette exchange using CRISPR/Cas9

Depositor

Richard Mann

Article

Voutev et al G3 (Bethesda). 2018 Mar 9. pii: g3.118.2

Insert

PhiC31 attP sites

Use

CRISPR

Available Since

June 12, 2018

Availability

Academic Institutions and Nonprofits only

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pRAU168

Plasmid

#86834

Purpose

bacterial expression of anti-CRISPR protein AcrIIA1

Depositor

Joseph Bondy-Denomy

Article

Rauch et al Cell. 2016 Dec 22. pii: S0092-8674(16)31

Insert

acrIIA1

Expression

Bacterial

Promoter

araBAD

Available Since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pEntr_PE2

Plasmid

#149548

Purpose

Gateway entry clone with PE2 enzyme (with stop codon)

Depositor

Norbert Perrimon

Article

Bosch et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

Insert

PE2

Use

CRISPR; Gateway shuttle vector

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCSW21

Plasmid

#86836

Purpose

bacterial expression of anti-CRISPR protein AcrIIA4

Depositor

Joseph Bondy-Denomy

Article

Rauch et al Cell. 2016 Dec 22. pii: S0092-8674(16)31

Insert

acrIIA4

Expression

Bacterial

Promoter

araBAD

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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p3xP3-EGFP.vas-int.NLS

Plasmid

#60948

Purpose

Drosophila, p3xP3-EGFP.vas-int.NLS.attB was digested SpeI to get rid of the attB site. used for RMCE. CRISPR.

Depositor

Frank Schnorrer

Article

Zhang et al G3 (Bethesda). 2014 Oct 15. pii: g3.114.

Insert

vas-phiC31 integrase.NLS

Use

Rmce

Promoter

vasa

Available Since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only
pMB1609_pRR-EGFP

Plasmid

#65852

Purpose

EGFP-based homologous recombination-dependent reporter for genome editing with TALENs or CRISPR/Cas9

Depositor

Marc Bühler

Article

Flemr et al Cell Rep. 2015 Jul 28;12(4):709-16. doi:

Insert

split EGFP coding sequence

Use

CRISPR and TALEN

Expression

Mammalian

Promoter

SV40

Available Since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pCAH01Sp::Cas9D10A

Plasmid

#128161

Purpose

Ptet-controlled expression of S. pyogenes Cas9D10A variant

Depositor

Calvin Henard

Article

Tapscott et al Appl Environ Microbiol. 2019 May 16;85(1

Insert

Sp Cas9

Expression

Bacterial

Mutation

D10A

Promoter

Ptet

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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pDD121

Plasmid

#91833

Purpose

Cas9 expression in C. elegans

Depositor

Bob Goldstein

Article

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

Cas9

Use

CRISPR

Expression

Worm

Mutation

Codon optimized and with synthetic introns for C.…

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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