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We narrowed to 16,183 results for: nes

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Showing: 10481 - 10500 of 16183 results

  2. TRE3GV-SpdCas9-Dnmt3A/3L-catΔ

    Plasmid
    #222508
    Purpose
    Expresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant catΔ (C706A and R832E) and hPGK promoter driven mCherry
    Depositor
    Isaac Hilton
    Publication
    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021
    Inserts
    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant catΔ (C706A and R832E) engineered fusion
    mCherry
    Use
    Lentiviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; C706A and R832…
    Promoter
    TRE3GV and hPGK
    Available since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    222508-plasmid-map-sequence-id-441225

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  3. pcDNA-dCas9-p300 Core (C1204R)

    Plasmid
    #61361
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; mutation C1204R) driven by CMV promoter
    Depositor
    Charles Gersbach
    Publication
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, S. pyogenes, Human)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; C1204R mutation i…
    Promoter
    CMV
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61361-plasmid-map-sequence-id-99794

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  4. pcDNA-dCas9-p300 Core (1396/1397 SY/WW)

    Plasmid
    #61363
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation 1396/1397 SY/WW) driven by CMV promoter
    Depositor
    Charles Gersbach
    Publication
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, S. pyogenes, Human)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; 1396/1397 SY/WW m…
    Promoter
    CMV
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61363-plasmid-map-sequence-id-99798

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  6. pGIPZ EF1a spIgE-3xFLAG-Neo2/15 IRES mNeonGreen-P2A-Puro

    Plasmid
    #248034
    Purpose
    Constitutively expresses 3xFLAG tagged Neoleukin 2/15 with an IgE signal peptide, mNeonGreen and a puromycin resistance gene in human cells
    Depositor
    Joshua Leonard
    Publication
    Deol et al GEN Biotechnol. 2023 Jun 1;2(3):228-246.
    Insert
    3xFLAG-tagged Neoleukin-2/15
    Use
    Lentiviral
    Tags
    3xFLAG
    Expression
    Mammalian
    Promoter
    human EF1a
    Available since
    Jan. 16, 2026
    Availability
    Academic Institutions and Nonprofits only
    248034-plasmid-map-sequence-id-497728

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  7. pIDTSmart-CMV-PLRS1

    Plasmid
    #217365
    Purpose
    Expression of previously developed E. coli leucyl aminoacyl-tRNA synthesis variant that is "polyspecific"
    Depositor
    Abhishek Chatterjee
    Publication
    Huang et al ACS Synth Biol. 2024 Jul 19;13(7):2141-2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    leucyl-tRNA synthetase polyspecific mutant
    Expression
    Mammalian
    Mutation
    M40I, T252A, Y499I, Y527A, H529G
    Promoter
    CMV
    Available since
    July 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    217365-plasmid-map-sequence-id-430948

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  8. CaMK2rep-S603A

    Plasmid
    #239623
    Purpose
    Inactive CaMKII activity reporter. The plasmid contains sGFP2 fluorescent protein, 3xmyc tags and double mutated phospho-site 3 from rat Synapsin-1a.
    Depositor
    F Javier Díez Guerra
    Publication
    Martinez-Blanco et al Anal Chem. 2025 Sep 23;97(37):20275-2029
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpotNES-sGFP2-SynP3-3xmyc (Syn1 Synthetic, Rat)
    Tags
    Spot, myc, and sGFP2
    Expression
    Mammalian
    Mutation
    changed Serine 603 to Alanine
    Promoter
    CMV
    Available since
    July 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    239623-plasmid-map-sequence-id-476237

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  9. AiP13657 - pAAV-AiE0655m-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN3657)

    Plasmid
    #230163
    Purpose
    AiE0655m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells
    Depositor
    Allen Institute for Brain Science, Boaz Levi
    Publication
    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.
    Insert
    SYFP2-P2A-3XFLAG-10aa-H2B
    Use
    AAV
    Promoter
    minBG
    Available since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    230163-plasmid-map-sequence-id-461888

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  10. AiP13635 - pAAV-AiE0387m-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN3635)

    Plasmid
    #230159
    Purpose
    AiE0387m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells
    Depositor
    Allen Institute for Brain Science, Boaz Levi
    Publication
    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.
    Insert
    SYFP2-P2A-3XFLAG-10aa-H2B
    Use
    AAV
    Promoter
    minBG
    Available since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    230159-plasmid-map-sequence-id-461884

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  11. AiP13294 - pAAV-AiE0940m-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN3294)

    Plasmid
    #230144
    Purpose
    AiE0940m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells
    Depositor
    Allen Institute for Brain Science, Boaz Levi
    Publication
    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.
    Insert
    SYFP2-P2A-3XFLAG-10aa-H2B
    Use
    AAV
    Promoter
    minBG
    Available since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    230144-plasmid-map-sequence-id-461871

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  12. AiP13295 - pAAV-AiE0941m-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN3295)

    Plasmid
    #230145
    Purpose
    AiE0941m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells
    Depositor
    Allen Institute for Brain Science, Boaz Levi
    Publication
    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.
    Insert
    SYFP2-P2A-3XFLAG-10aa-H2B
    Use
    AAV
    Promoter
    minBG
    Available since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    230145-plasmid-map-sequence-id-461872

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  14. pLV-SFFV-CALU-WPRE-UbC-Emerald

    Plasmid
    #225951
    Purpose
    Lentiviral vector plasmid expressing human calumenin (CALU) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Richard Eva, Bart Nieuwenhuis, Evan Reid
    Inserts
    CALU (CALU Human)
    Emerald
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    SFFV and UbC
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    225951-plasmid-map-sequence-id-446853

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  15. AiP13655 - pAAV-AiE0552h-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN3655)

    Plasmid
    #224035
    Purpose
    AiE0552h is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L5_IT and L6_IT neurons
    Depositor
    Allen Institute for Brain Science, Jonathan Ting
    Publication
    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.
    Insert
    SYFP2-P2A-3XFLAG-10aa-H2B
    Use
    AAV
    Mutation
    NA
    Promoter
    minBG
    Available since
    Oct. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    224035-plasmid-map-sequence-id-449734

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  16. mCh-LgBiT-2A-EGFP-Vin-PILATeS-CT

    Plasmid
    #216763
    Purpose
    PiggyBac vector for stable co-expression of mCherry-LgBiT and EGFP-Vinculin-PILATeS-CT (700 pM) negative control in mammalian cells. Requires transposase
    Depositor
    Alex Dunn
    Publication
    Zhong et al ACS Sens. 2024 Jul 26;9(7):3489-3495. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry-LgBiT-P2A-T2A-EGFP-Vin-PILATeS-CT
    Tags
    EGFP and mCherry
    Expression
    Mammalian
    Available since
    Aug. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    216763-plasmid-map-sequence-id-428945

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  17. pYB-Dual(mNG(yeast-opt))

    Plasmid
    #216727
    Purpose
    Dual-species protein expression vector for high-level and inducible protein expression of mNeonGreen (codon optimized for yeast) in both yeast and bacteria from the same construct.
    Depositor
    Emil Jensen
    Publication
    Waneskog et al Biotechnol Prog. 2024 May 17:e3482. doi:
    Insert
    mNeonGreen
    Expression
    Bacterial and Yeast
    Promoter
    pGAL1(yeast) and pT7-RNAP(bacteria)
    Available since
    May 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    216727-plasmid-map-sequence-id-430980

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  18. iLID-tdTomato-Omp25

    Plasmid
    #214400
    Purpose
    Expresses fusion of iLID with tdTomato and transmembrane domain of Omp25
    Depositor
    Liting Duan
    Publication
    Liu et al Proc Natl Acad Sci U S A. 2024 Apr 2;121
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    iLID-tdTomato-Omp25 (Synj2bp Synthetic, Rat)
    Tags
    iLID-TdTomato-Omp25
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    214400-plasmid-map-sequence-id-426102

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  19. MultiMate-HITI-2c ACTB

    Plasmid
    #206267
    Purpose
    All in one recombinant baculovirus production vector. Encodes Cas9, sgRNA and donor for homology independent targeted integration in the ACTB locus.
    Depositor
    Imre Berger
    Publication
    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Insert
    SpCas9 (S. Pyogenes), hACTB HITI-2c donor, mCherry, Puro-R, hU6 hACTB sgRNA, AcrIIA4, eGFP
    Use
    CRISPR; Recombinant baculovirus production
    Expression
    Mammalian
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    206267-plasmid-map-sequence-id-406623

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Showing: 10481 - 10500 of 16183 results