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-226bp goosecoid luciferase

Plasmid

#17158

Depositor

Ken Cho

Article

Watabe et al Genes Dev. 1995 Dec 15. 9(24):3038-50.

Insert

goosecoid promoter (gsc-a Frog)

Use

Luciferase

Tags

luciferase

Available Since

June 20, 2008

Availability

Academic Institutions and Nonprofits only
pGL3con-miR143Bul

Plasmid

#21119

Depositor

Joshua Mendell

Article

Hwang et al Proc Natl Acad Sci U S A. 2009 Apr 28;10

Insert

hsa-miR-143 sensor

Expression

Mammalian

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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pCMV-Flag-YAP-P85A

Plasmid

#33062

Depositor

Kunliang Guan

Article

Li et al Genes Dev. 2010 Feb 1;24(3):235-40.

Insert

YAP (YAP1 Human)

Tags

Flag

Expression

Mammalian

Mutation

P85A

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
pISH-Emx1

Plasmid

#84309

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Emx1. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Emx1 (Emx1 Mouse)

Available Since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only

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MSCV-PM-BAF180-SH2

Plasmid

#41932

Depositor

Stephen Elledge

Article

Burrows et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

Insert

BAF180

Use

Retroviral

Expression

Mammalian

Available Since

May 10, 2013

Availability

Academic Institutions and Nonprofits only
1xNLS-pMJ915v2-sfGFP

Plasmid

#88919

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Article

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Inserts

Cas9
T7

Use

CRISPR

Expression

Bacterial

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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Hy_pMT dati MCS circfirefly_1677

Plasmid

#110118

Purpose

Expresses a 1677-nt firefly luciferase circular RNA in Drosophila cells

Depositor

Jeremy Wilusz

Article

Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

Insert

Firefly luciferase

Expression

Insect

Promoter

Metallothionein Promoter (pMT)

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pGL3_ctrl_CP-candidate_luc+

Plasmid

#86392

Purpose

Luciferase validation vector, where candidates are inserted at the position of the core promoter. The candidates basal activity (no enhancer in vector) is reflected by luciferase activity

Depositor

Alexander Stark

Article

Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

Type

Empty backbone

Use

Luciferase

Available Since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
2xNLS-pMJ915v2-sfGFP

Plasmid

#88920

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Article

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Inserts

Cas9
T7

Use

CRISPR

Expression

Bacterial

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pGEM cBspII (CT#624)

Plasmid

#13940

Depositor

Cliff Tabin

Article

Hartmann et al Development. 2000 Jul . 127(14):3141-59.

Insert

BspII (IBSP Chicken)

Expression

Bacterial

Mutation

Amino acids 79-257

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
pWZL Hygro- TRF2 deltaM

Plasmid

#18012

Depositor

Titia de Lange

Article

Karlseder et al Science. 2002 Mar 29. 295(5564):2446-9.

Insert

hTRF2 (TERF2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

Deleted Myb Domain (aa454-500)

Available Since

May 29, 2008

Availability

Academic Institutions and Nonprofits only
pPS1358

Plasmid

#8918

Depositor

Pamela Silver

Article

Kessler et al Genes Dev. 1997 Oct 1. 11(19):2545-56.

Insert

HRP1 (HRP1 Budding Yeast)

Tags

GFP

Expression

Yeast

Available Since

Aug. 19, 2005

Availability

Academic Institutions and Nonprofits only

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pNH030

Plasmid

#42149

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

mTFP1::linker::2xNLS

Expression

Worm

Available Since

Feb. 5, 2013

Availability

Academic Institutions and Nonprofits only

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TALEN-ID12-T

Plasmid

#41479

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Biotechnol. 2012 Dec 16. doi: 10.103

Insert

TALEN-FokI nuclease backbone

Tags

3xFLAG and WT FokI nuclease domain

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only

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-1.5 kb goosecoid luciferase

Plasmid

#17159

Depositor

Ken Cho

Article

Watabe et al Genes Dev. 1995 Dec 15. 9(24):3038-50.

Insert

goosecoid promoter (gsc-a Frog)

Use

Luciferase

Tags

luciferase

Available Since

June 20, 2008

Availability

Academic Institutions and Nonprofits only
pGEM beta catenin (CT#593)

Plasmid

#13938

Depositor

Cliff Tabin

Article

Hartmann et al Development. 2000 Jul . 127(14):3141-59.

Insert

beta catenin (CTNNB1 Chicken)

Expression

Bacterial

Mutation

Amino acids 64-272

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
pISH-Tshz3

Plasmid

#74362

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Tshz3. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

eashirt zinc finger family member 3 (Tshz3 Mouse)

Available Since

April 13, 2016

Availability

Academic Institutions and Nonprofits only

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pBRIT Pax7 N33-170C

Plasmid

#17526

Depositor

Michael Rudnicki

Article

McKinnell et al Nat Cell Biol. 2008 Jan . 10(1):77-84.

Insert

Pax7 (Pax7 Mouse)

Use

Cre/Lox and Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Deletion of aa 34-159 (paired domain)

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pBRIT Pax7 N215-273C

Plasmid

#17527

Depositor

Michael Rudnicki

Article

McKinnell et al Nat Cell Biol. 2008 Jan . 10(1):77-84.

Insert

Pax7 (Pax7 Mouse)

Use

Cre/Lox and Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Deletion of aa 216-272 (homeodomain)

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
CENTG3 (3IHW)

Plasmid

#25159

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

CENTG3:P126-K291 (AGAP3 Human)

Tags

His

Expression

Bacterial

Mutation

Contains P126-K291

Available Since

Aug. 6, 2010

Availability

Industry, Academic Institutions, and Nonprofits