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pDonor_hU6

Plasmid

#69312

Purpose

sgRNA scaffold and human U6 promoter

Depositor

Andrea Ventura

Article

Vidigal et al Nat Commun. 2015 Aug 17;6:8083. doi: 10.

Insert

hU6 promoter and sgRNA scaffold flanked by BbsI sites

Use

CRISPR

Promoter

human U6 promoter

Available Since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pSC142

Plasmid

#122835

Purpose

SUC2 promoter with GreenGate A-B flanking sequences

Depositor

Markus Schmid

Article

Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do

Insert

SUC2 promoter

Use

Golden gate compatible cloning vector

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pPEE7

Plasmid

#128357

Purpose

Recycable marker for use in Cryptococcus neoformans. Contains the amdS1 counterselectable marker flanked by direct repeats.

Depositor

James Fraser

Article

Erpf et al Fungal Genet Biol. 2019 Jun 17;131:10324

Type

Empty backbone

Use

Unspecified

Promoter

ACT1

Available Since

Oct. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pPEE8

Plasmid

#128358

Purpose

Recycable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by direct repeats.

Depositor

James Fraser

Article

Erpf et al Fungal Genet Biol. 2019 Jun 17;131:10324

Type

Empty backbone

Use

Unspecified

Promoter

TEF1

Available Since

Oct. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pNJB80

Plasmid

#51518

Purpose

Gateway entry vector with attL5 and attL2 sites flanking a CmR and ccdB gene. In our hands, the multisite reaction still proceeds efficiently.

Depositor

Daniel Voytas

Article

Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

Type

Empty backbone

Use

Gateway entry vector

Available Since

March 6, 2014

Availability

Academic Institutions and Nonprofits only

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pPL5617_pUG_PuroR

Plasmid

#60928

Purpose

PCR template vector for generating puromycin resistance cassette.

Depositor

Robert Piper

Article

MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

Insert

puromycin N-Acetyltransferase

Use

Loxp flanked deletion cassette template

Promoter

TEF1

Available Since

Feb. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pDG058

Plasmid

#122840

Purpose

SRDX repressor domain with GreenGate D-E flanking sequences

Depositor

Markus Schmid

Article

Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do

Insert

SRDX transcriptional repressor domain

Use

Golden gate compatible cloning vector

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pMH185

Plasmid

#122839

Purpose

VP64 activator domain with GreenGate D-E flanking sequences

Depositor

Markus Schmid

Article

Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do

Insert

VP64 transcriptional activator domain

Use

Golden gate compatible cloning vector

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pNIC-TolB-NHSTIIT-GG

Plasmid

#215792

Purpose

Cloning of target gene with N terminal TolB SRP pathway signal peptide N terminal His6 Twin Strep tag and TEV cleavage

Depositor

ASAP Discovery, Frank von Delft

Article

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Expression

Bacterial

Available Since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pNIC-PelB-NH10T-AVITAG-GG

Plasmid

#215793

Purpose

Cloning of target gene with N terminal PelB SEC pathway signal peptide His10 and AVITAG and TEV cleavage

Depositor

ASAP Discovery, Frank von Delft

Article

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Expression

Bacterial

Available Since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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