We narrowed to 18,112 results for: Eomes

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pTS1051-Tier1-PhCMV-4xCox8

Plasmid

#169490

Purpose

Tier-1 vector encoding PhCMV-driven four-fold repeat of a mitochondrial matrix localization signal derived from mitochondrial matrix protein Cox8 (PhCMV-4xCox8-pA)

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven mitochondrial localization signal from cytochrome c oxidase subunit 8

Expression

Mammalian

Promoter

PhCMV

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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YFP-DAXX-mSIM1

Plasmid

#134524

Purpose

Expresses YFP-DAXX fusion protein with a mutation in its 5' SIM

Depositor

Susan Janicki

Article

Shastrula et al J Cell Sci. 2019 Mar 26;132(6). pii: jcs

Insert

DAXX-mSIM1

Tags

YFP

Expression

Mammalian

Mutation

SIM (IIVL) 7-10 aa changed to IGAG

Promoter

CMV

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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(424) pcDNA3.1(+)-HA-LaG6-(EAAAK)4-OGT(4.5, K852A)

Plasmid

#168191

Purpose

HA-tagged GFP nanobody Lag6 (310 nM affinity) fused to an inactive truncated OGT of 4 TPRs with K852A mutation

Depositor

Christina Woo

Article

Ramirez et al Curr Protoc. 2021 May;1(5):e117. doi: 10

Insert

HA-nLag6-(EAAAK)4-OGT(4,K852A)

Tags

HA-Tag

Expression

Mammalian

Promoter

T7/CMV

Available Since

May 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJAF215

Plasmid

#52402

Purpose

Expresses Flag-tagged mouse Arf1

Depositor

Gregory Pazour

Article

Follit et al PLoS Genet. 2014 Feb 20;10(2):e1004170.

Insert

Arf1 (Arf1 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

April 14, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-CibN-dCas9

Plasmid

#60551

Purpose

Expresses CibN-dCas9 in mammalian cells

Depositor

Charles Gersbach

Article

Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

Insert

CibN-dCas9

Use

CRISPR

Tags

FLAG and SV40 NLS

Expression

Mammalian

Mutation

inactivating Cas9 mutations D10A and H840A

Promoter

CMV

Available Since

Feb. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pMpGWB435

Plasmid

#68700

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

TagRFP

Expression

Plant

Promoter

Cauliflower mosaic virus 35S promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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EGFP-ER-14

Plasmid

#56432

Purpose

Localization: Endoplasmic Reticulum, Excitation: 488, Emission: 507

Depositor

Michael Davidson

Article

Day et al Chem Soc Rev. 2009 Oct;38(10):2887-921.

Insert

ER (CALR Human)

Tags

EGFP

Expression

Mammalian

Mutation

ER Signal Peptide

Promoter

CMV

Available Since

Dec. 23, 2014

Availability

Industry, Academic Institutions, and Nonprofits
GPBA-Tango

Plasmid

#66298

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

GPBA

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
rTetR-GFP-VP16

Plasmid

#183917

Purpose

VP16 activation domain coupled to reverse Tet repressor; labeled with GFP; binds tetO sites upon doxycycline addition

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

rTetR-GFP-VP16 (UL48 Synthetic)

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 Flag-Sesn2

Plasmid

#61868

Purpose

Transient overexpression of Flag-Sesn2 (mouse)

Depositor

Ming Li

Article

Peng et al Cell. 2014 Sep 25;159(1):122-33. doi: 10

Insert

Sesn2 (Sesn2 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only
shHIF2α(#2) (HIF2α-#3-pSUPER-retro-GFP/Neo)

Plasmid

#22132

Depositor

William Kaelin

Article

Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

Insert

synthethic oligonucleotide

Use

RNAi and Retroviral

Expression

Mammalian

Mutation

synthethic oligonucleotide encoding shRNA targeti…

Available Since

Feb. 1, 2010

Availability

Academic Institutions and Nonprofits only

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pCR3.1 GSK3beta S9A HA

Plasmid

#15994

Depositor

Morris Birnbaum

Article

Summers et al J Biol Chem. 1999 Jun 18. 274(25):17934-

Insert

GSK3beta (GSK3B Human)

Tags

HA

Expression

Mammalian

Mutation

S9A: constitutively active

Available Since

Oct. 26, 2007

Availability

Academic Institutions and Nonprofits only
1199 pAdTrack FKHR AAA

Plasmid

#9037

Depositor

William Sellers

Article

Ramaswamy et al Cancer Cell. 2002 Jul . 2(1):81-91.

Insert

FKHR AAA (FOXO1 Human)

Use

Adenoviral

Tags

flag

Expression

Mammalian

Mutation

T24A S256A S319A

Available Since

Sept. 28, 2005

Availability

Academic Institutions and Nonprofits only
Tmem98-mCherry

Plasmid

#124505

Purpose

Expresses mCherry-tagged mouse TMEM98 in mammalian cells (C-terminal tag)

Depositor

Renee van Amerongen

Article

van der Wal et al PLoS One. 2020 Jan 21;15(1):e0227435. do

Insert

Tmem98 (Tmem98 Mouse)

Tags

mCherry

Expression

Mammalian

Mutation

stop codon was removed to allow fusion with sGFP2…

Promoter

CMV

Available Since

May 12, 2020

Availability

Academic Institutions and Nonprofits only

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YFP-Rac1(L61)

Plasmid

#11401

Depositor

Joel Swanson

Article

Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

Insert

ras-related C3 botulinum toxin substrate 1 (RAC1 Human)

Tags

YFP

Expression

Mammalian

Mutation

Obtained with Glutamine 61 mutated to Leucine.

Available Since

Feb. 21, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3.2/V5 hsa-mir-192

Plasmid

#26311

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

hsa-mir-192 (MIR192 Human)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3-Lyn-μDpTT

Plasmid

#182321

Purpose

Plasma membrane-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.

Depositor

Jin Zhang

Article

Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

Insert

Lyn-μDronpa-TagRFP-T

Tags

N-terminal targeting sequence from Lyn kinase

Expression

Mammalian

Mutation

Dronpa contains H193T mutation.

Promoter

CMV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-CYP450-μDpTT

Plasmid

#182325

Purpose

Endoplasmic reticulum-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.

Depositor

Jin Zhang

Article

Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

Insert

DAKAP-μDronpa-TagRFP-T

Tags

N-terminal 27 amino acids from CYP450

Expression

Mammalian

Mutation

Dronpa contains H193T mutation.

Promoter

CMV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lifeact-μDpTT

Plasmid

#182322

Purpose

Actin-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.

Depositor

Jin Zhang

Article

Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

Insert

Lifeact-μDronpa-TagRFP-T

Tags

N-terminal 17 amino acids from budding yeast Abp1…

Expression

Mammalian

Mutation

Dronpa contains H193T mutation.

Promoter

CMV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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optoPKAW196R/Y204A

Plasmid

#104546

Purpose

Expresses Cry2-mCherry-PKA W196R/Y204A in mammalian cells.

Depositor

David Lawrence

Article

O'Banion et al Cell Chem Biol. 2017 Oct 25. pii: S2451-

Insert

Cry2-mCherry-PKA W196R/Y204A

Tags

mCherry

Expression

Mammalian

Mutation

PKA W196R and Y204A

Promoter

CMV

Available Since

March 12, 2018

Availability

Academic Institutions and Nonprofits only

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