We narrowed to 29,291 results for: REP

1. pRL-TK 4x mut H

   Plasmid

   #11321

   Depositor

   Phil Sharp

   Article

   Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

   Insert

   4 bulged binding sites for CXCR4 siRNA antisense
   

   Use

   Luciferase

   Tags

   Rr-luc

   Expression

   Mutation

   Mutation H (see paper) on inner 2 binding sites.

   Promoter

   Available since

   Feb. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

2. pRL-TK 4x mut GFP

   Plasmid

   #11323

   Depositor

   Phil Sharp

   Article

   Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

   Insert

   2 bulged binding sites for CXCR4 siRNA antisense, flanking 2 binding sites for GFP
   

   Use

   Luciferase

   Tags

   Rr-luc

   Expression

   Mutation

   Promoter

   Available since

   Feb. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

3. pCBASceI

   Plasmid

   #26477

   Purpose

   I-SceI endonuclease expression vector with mammalian promoter to introduce a DSB at a genomic I-SceI site

   Depositor

   Maria Jasin

   Article

   Richardson et al Genes Dev. 1998 Dec 15. 12(24):3831-42.

   Insert

   pCBASceI
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 20, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLX-EF1a_Empty

   Plasmid

   #221480

   Purpose

   Empty vector control for pLX307 overexpresion experiments

   Depositor

   Matthew Meyerson

   Article

   Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLX-EF1a_mCherry

   Plasmid

   #221482

   Purpose

   mCherry lentiviral overexpression

   Depositor

   Matthew Meyerson

   Article

   Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

   Insert

   mCherry
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. CROPseq-EFS-HypaSpCas9-P2A-puro

   Plasmid

   #106965

   Purpose

   single-vector CROPseq system with high fidelity SpCas9 (HypaSpCas9) for use in the CROPseq CRISPR knockout screen

   Depositor

   Alex Hewitt

   Insert

   HypaSpCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   April 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFR_HCV_xb

   Plasmid

   #11510

   Depositor

   Phil Sharp

   Article

   Petersen et al Mol Cell. 2006 Feb 17. 21(4):533-42.

   Insert

   Firefly luciferase, Hepatitis C Virus IRES sequence, Renilla, and 3'UTR short RNA binding sites
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 25, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. FLX1.8NFATGFPpd2HS4

   Plasmid

   #169096

   Purpose

   Lentiviral vector encoding a short-lived GFP under the transcriptional control of a mutlimerized NFAT gene enhancer to measure T cell activation

   Depositor

   Geoffrey Owens

   Inserts

   NFAT enhancer (from IL-4 promoter)
   Destabilized green fluorescent protein
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   NFAT enhancer + synthetic TATA box

   Available since

   July 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. CROPseq-EFS-HypaSpCas9-P2A-EGFP

   Plasmid

   #106964

   Purpose

   single-vector CROPseq system with high fidelity SpCas9 (HypaSpCas9) for use in the CROPseq CRISPR knockout screen

   Depositor

   Alex Hewitt

   Insert

   HypaSpCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. EC67841

    Plasmid

    #209512

    Purpose

    Cloning in complete guide expression cassettes, with Cas12a (D156R)

    Depositor

    Cristobal Uauy, Wendy Harwood

    Article

    Lawrenson et al Research Square, 12 March 2024, PREPRINT

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMSCVhygro-Cre

    Plasmid

    #34565

    Depositor

    Kai Ge

    Article

    Wang et al Proc Natl Acad Sci U S A. 2010 Apr 20. 1

    Insert

    Cre
    

    Use

    Cre/Lox and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    5'LTR

    Available since

    March 28, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. Rosa26 mT/mG

    Plasmid

    #17787

    Depositor

    Liqun Luo

    Article

    Muzumdar et al Genesis. 2007 Sep . 45(9):593-605.

    Insert

    Rosa26 mT/mG
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    membrane tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFGL821

    Plasmid

    #58223

    Purpose

    For fungal transformation via ATMT. Transformants to be selected by hygromicin resistance.

    Depositor

    Naweed Naqvi

    Article

    Sweigard et al Fungal Genetics Reports, 1997. Vol. 44,

    Insert

    hygromycin B phosphotransferase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Aspergillus nidulans trpC promoter

    Available since

    May 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. dCas9-miniTurbo

    Plasmid

    #219822

    Purpose

    EF1α-driven expression of dCas9 fused to miniTurbo with GFP (Adapted from plasmid #153209)

    Depositor

    Philipp Maass

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    miniTurbo

    Expression

    Mammalian

    Mutation

    D10A/H841A

    Promoter

    EF1a

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCFT4.2

    Plasmid

    #208807

    Purpose

    Expresses the fluorescent protein phiLOV2.1

    Depositor

    François-Xavier Campbell-Valois

    Article

    Tchagang et al Front Microbiol. 2023 Oct 20;14:1245755.

    Insert

    phiLOV2.1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Oct. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. EC64420

    Plasmid

    #209517

    Purpose

    Cloning in complete guide expression cassettes, with Cas9

    Depositor

    Cristobal Uauy, Wendy Harwood

    Article

    Lawrenson et al Research Square, 12 March 2024, PREPRINT

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pOpen-T4 g32

    Plasmid

    #165560

    Purpose

    A single-stranded DNA binding protein required for bacteriophage T4 replication and repair; Used extensively to stabilize and mark regions of ssDNA for electron microscopic examination of intracellular DNA structure, to increase yield and processivity of reverse transcriptase during RT-PCR, and to increase yield and specificity of PCR products from soil samples.

    Depositor

    Drew Endy, Jennifer Molloy, FreeGenes Project

    Insert

    T4 gene 32
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 16, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pCMV-GAL4

    Plasmid

    #24345

    Depositor

    Liqun Luo

    Article

    Potter et al Cell. 2010 Apr 30. 141(3):536-48.

    Insert

    GAL4
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pIS2

    Plasmid

    #12177

    Depositor

    David Bartel

    Article

    Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

    Type

    Empty backbone

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHAGE mCherry-Rab11A

    Plasmid

    #228035

    Purpose

    Mammalian lentiviral expression of mCherry-labeled Rab11.

    Depositor

    Wade Harper

    Insert

    Rab11A (RAB11A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge