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pDONR221_SLC26A3

Plasmid

#132020

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC26A3 (SLC26A3 Human)

Expression

Mammalian

Available Since

Oct. 25, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SLC25A39

Plasmid

#131970

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC25A39 (SLC25A39 Human)

Expression

Mammalian

Available Since

Oct. 18, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SLC12A3

Plasmid

#131920

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC12A3 (SLC12A3 Human)

Expression

Mammalian

Available Since

Oct. 15, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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GFP-HDEL

Plasmid

#129098

Purpose

For labeling endoplasmic reticulum (ER) in S. cerevisiae with GFP-HDEL

Depositor

Wei-Lih Lee

Article

Omer et al Elife. 2018 Aug 7;7. pii: 36745. doi: 10

Insert

TEF1p-SS-3xGlyAla-GFP-HDEL

Expression

Yeast

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-MAP3K4

Plasmid

#116759

Purpose

Lentiviral expression of MAP3K4

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

MAP3K4 (MAP3K4 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-BMPR2

Plasmid

#116718

Purpose

Lentiviral expression of BMPR2

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BMPR2 (BMPR2 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pMRE162

Plasmid

#118510

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

sGFP2

Expression

Bacterial

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pMRE160

Plasmid

#118508

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

TagBFP2

Expression

Bacterial

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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6x Halo-EGFP- 2x PACT

Plasmid

#107265

Purpose

6x Halo-EGFP- 2x PACT construct downstream of T7 promotor for in vitro transcription

Depositor

Michael Lampson

Article

Akera et al Science. 2017 Nov 3;358(6363):668-672. d

Insert

6x Halo-EGFP- 2x PACT

Tags

GFP

Expression

Mammalian

Promoter

T7

Available Since

July 4, 2018

Availability

Academic Institutions and Nonprofits only

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[Bla][IRES][GFP]

Plasmid

#97193

Purpose

Vector for our translation quantification assay (Fig. 5). Double reporter vector with internal ribosome entry site.

Depositor

Edward Boyden

Article

Adamala et al Proc Natl Acad Sci U S A. 2016 May 10;11

Inserts

beta-Lactamase
GFP

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pSpal2At

Plasmid

#78286

Purpose

Expresses A. thaliana PAL2 in E. coli

Depositor

David Nielsen

Article

McKenna et al Metab Eng. 2011 Sep;13(5):544-54. doi: 1

Insert

PAL2 (PAL2 Mustard Weed)

Expression

Bacterial

Mutation

*see below

Promoter

lac

Available Since

June 1, 2016

Availability

Academic Institutions and Nonprofits only

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pJZC504

Plasmid

#62280

Purpose

PCP-VP64 fusion

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Insert

PCP-VP64

Tags

VP64

Expression

Yeast

Promoter

pAdh

Available Since

March 17, 2015

Availability

Academic Institutions and Nonprofits only

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mEmerald-Talin-C-18

Plasmid

#62763

Purpose

Localization: Talin/Focal Adhesions, C1 Cloning Vector, Excitation:487, Emission:509

Depositor

Michael Davidson

Insert

Talin

Tags

mEmerald

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

March 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pAF-HdrB3

Plasmid

#184908

Purpose

carrying CRISPR/Cas9 system for genome editing in Acidithiobacillus ferridurans

Depositor

Radhakrishnan Mahadevan

Article

Chen et al Environ Sci Technol. 2023 Aug 22;57(33):

Insert

Cas9-sgRNA-homology arms

Use

CRISPR; Broad-host-range

Expression

Bacterial

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Tornado-F30-Squash-Broccoli

Plasmid

#177918

Purpose

Expresses a bifunctional circular RNA with Squash in one arm and Broccoli in the other arm of the F30 scaffold, in mammalian cells, using the Tornado expression system.

Depositor

Samie Jaffrey

Article

Dey et al Nat Chem Biol. 2021 Dec 22. pii: 10.1038

Insert

Tornado-F30-Squash-Broccoli

Expression

Mammalian

Promoter

U6+27

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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Sar1b-H79G-mOrange2

Plasmid

#166900

Purpose

mammalian expression of Sar1b-H79G tagged with mOrange2

Depositor

Jennifer Lippincott-Schwartz

Article

Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

Insert

Sar1b

Tags

mOrange2

Expression

Mammalian

Mutation

H79G

Promoter

CMV

Available Since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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pNHEJ-Cas12a-recX

Plasmid

#158714

Purpose

CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium smegmatis. Helper plasmid expresses FnCpf1, NHEJ machinery of M. marinum and recX

Depositor

Yi-Cheng Sun

Article

Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

Insert

recX

Use

CRISPR

Expression

Bacterial

Promoter

Pmyc

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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HA-GLUT5 in pRK5

Plasmid

#154205

Purpose

N-terminal HA-tagged GLUT5 cDNA expression vector for transfections

Depositor

David Sabatini

Article

Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

Insert

SLC2A5 (SLC2A5 Human)

Tags

N terminal HA tag

Expression

Mammalian

Available Since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pXF89

Plasmid

#139030

Purpose

MosSCI SapTrap assembly: Cassette 2 for 4- or 5-cassette system

Depositor

Erik Griffin

Article

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

halotag (no STOP codon, PATC introns)

Expression

Worm

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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OSCA1.1

Plasmid

#136592

Purpose

Expresses the mechanosensitive channel OSCA1.1 in mammalian cells

Depositor

Ardem Patapoutian

Article

Murthy et al Elife. 2018 Nov 1;7. pii: 41844. doi: 10

Insert

OSCA1.1 (AT4G04340 Mustard Weed)

Tags

pIRES2-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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