-
Plasmid#70232PurposeChEC-tagging vector encoding a 3xFLAG tag and aa 83-231 of Mnase in pFA6a-3HA- HIS3MX6 replacing the 3xHA tag. These vectors retain compatibility with the F2/R1 primer pairs commonly used for epitopeDepositorInsert3xFLAG tag and aa 83-231 of Mnase
UseYeast genomic targetingTagsExpressionMutationPromoterAvailable sinceNov. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q109
Plasmid#111730PurposeBaculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cellsDepositorInsertHTT (HTT Human)
UseBaculovirus expressionTagsFLAGExpressionMammalianMutationQ109 (polyQ repeat)PromoterCMV and P10Available sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
1099 pcDNA GFP FKHR AAA
Plasmid#9023DepositorInsertFKHR AAA (FOXO1 Human)
UseTagsGFPExpressionMammalianMutationT24A S256A S319APromoterAvailable sinceDec. 7, 2005AvailabilityAcademic Institutions and Nonprofits only -
FKBP-beta3(702-1094)-GFP
Plasmid#100734PurposeTo express a clathrin "hook" in mammalian cells to hot-wire endocytosisDepositorInsertAP3B1 (AP3B1 Human)
UseTagsFKBP and GFPExpressionMammalianMutationappendage and ear onlyPromoterCMVAvailable sinceSept. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-GFP-dynein-2(D1091–Q4307)
Plasmid#64064PurposeCodon-optimized human dynein-2 motor domain (D1091–Q4307) for baculovirus expression. 6His-ZZ-GFP N-terminal tag, TEV site to cleave 6His-ZZ.DepositorInsertCytoplasmic dynein-2 (DYNC2H1 Human)
UseTagsGFP, His tag, and ZZ tagExpressionInsectMutationDeleted amino acids 1-1090, extra C-terminal vali…PromoterPolyhedrinAvailable sinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Flag-YAP-4SA(S109)
Plasmid#33081DepositorInsertYAP (YAP1 Human)
UseTagsFlagExpressionMammalianMutationS61A, S127A, S164A, S381APromoterCMVAvailable sinceFeb. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag mTOR S2035T R2109A
Plasmid#26606DepositorInsertmTOR (MTOR Human)
UseTagsFlagExpressionMammalianMutationS2035T R2109APromoterAvailable sinceNov. 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET11a asyn Q109E (TAC-->TAT)
Plasmid#105735PurposeBacterial expression of mutant human alpha synucleinDepositorInsertalpha-synuclein Q109E (TAC-->TAT) (SNCA Human)
UseTagsExpressionBacterialMutationQ109EPromoterT7Available sinceMay 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET11a asyn Q109N (TAC-->TAT)
Plasmid#105737PurposeBacterial expression of mutant human alpha synucleinDepositorInsertalpha-synuclein Q109N (TAC-->TAT) (SNCA Human)
UseTagsExpressionBacterialMutationQ109NPromoterT7Available sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
Bcr GAP domain R1090A/pGEX 3X
Plasmid#36406DepositorInsertBcr GAP domain encoding residues 1004-1271 with R1090A point mutation (BCR Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJune 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGEX-parkin delta 101-109
Plasmid#227364PurposeFor bacterial expression of parkin with a mutated ACT.DepositorInsertParkin delta 101-109 (PRKN Human)
UseTagsGSTExpressionBacterialMutationCodon usage optimized for E.coli expression. Resi…PromoterAvailable sinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
MAC-C Sars-CoV-2 NSP6_S107-109del
Plasmid#194820PurposeSars-CoV-2 NSP6_S107-109del in MAC-C destination vectorDepositorInsertNSP6_S107-109del (ORF1ab Sars-CoV-2)
UseTagsMAC-CExpressionMammalianMutationS107-109delPromoterAvailable sinceMay 16, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
Anti-CALB1/calbindin [L109/39R-1]
Plasmid#206686PurposeMammalian Expression Plasmid of anti-CALB1/calbindin (Human). Derived from hybridoma L109/39-1.DepositorInsertanti-CALB1/calbindin (Homo sapiens) recombinant Mouse monoclonal antibody (CALB1 Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
Anti-CALB1/calbindin [L109/39R-2b]
Plasmid#206635PurposeMammalian Expression Plasmid of anti-CALB1/calbindin (Human). Derived from hybridoma L109/39-2b.DepositorInsertanti-CALB1/calbindin (Homo sapiens) recombinant Mouse monoclonal antibody (CALB1 Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-guide-puro mBltp1 (KIAA1109) - 1
Plasmid#198489Purposelentiviral stable expression of mBltp1 (KIAA1109) gRNA 1DepositorInsertmBltp1 (4932438A13Rik Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterU6Available sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-guide-puro mBltp1 (KIAA1109) - 2
Plasmid#198490Purposelentiviral stable expression of mBltp1 (KIAA1109) gRNA 2DepositorInsertmBltp1 (4932438A13Rik Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterU6Available sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-HsNot1iso1_1093-1317-K1208AH1212AK1218A_T
Plasmid#147574PurposeBacterial Expression of HsNot1iso1_1093-1317-K1208AH1212AK1218ADepositorInsertHsNot1iso1_1093-1317-K1208AH1212AK1218A (CNOT1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-lambdaN-HA-HsSmg7_633-1091-siRNAres_U
Plasmid#147646PurposeMammalian Expression of HsSmg7_633-1091-siRNAresDepositorInsertHsSmg7_633-1091-siRNAres (SMG7 Human)
UseTagsExpressionMammalianMutationtwo non silent mutations Q651R and V854I compared…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-lambdaN-HA-HsSmg7_236-1091-siRNAres_I
Plasmid#146533PurposeMammalian Expression of HsSmg7_236-1091-siRNAresDepositorInsertHsSmg7_236-1091-siRNAres (SMG7 Human)
UseTagsExpressionMammalianMutationtwo silent mutations G216A and A1521G and two non…PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLNHA-C1-HsNot1iso1_840-1605-del1097-1110_X
Plasmid#147998PurposeMammalian Expression of HsNot1iso1_840-1605-del1097-1110DepositorInsertHsNot1iso1_840-1605-del1097-1110 (CNOT1 Human)
UseTagsExpressionMammalianMutation1 silent mutation compared to the sequence of iso…PromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only