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Plasmid#195044PurposepFA6a derived selection cassette 5' flanked with tCYC1 transcription terminator, allows genome modification without disruption of insertion neighboring genes by transcription interferenceDepositorInsertNatR
UseYeast genomic targetingTagstCYC1 S. cerevisiae, pPGK1 C. glabrata, NatR, tPG…ExpressionMutationPromoterAvailable sinceFeb. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
FASTHDR-Cterm-NanoLuc-Puromycin
Plasmid#167209PurposePlasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with NanoLuc and selection with PuromycinDepositorTypeEmpty backboneUseCRISPRTagsNanoLucExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCfB2899(X-2 MarkerFree)
Plasmid#73271PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-2 (Chr X: 194944..195980)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
PAX6 sgRNA7
Plasmid#68466Purposetargeting PAX6 geneDepositorInsertsgRNA targeting PAX6 (PAX6 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCfB2903(XI-2 MarkerFree)
Plasmid#73275PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-2 (Chr XI: 91575..92913)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDN-G1Gh
Plasmid#44507DepositorInsertsUseSynthetic Biology; Expression regulator/reporterTagsExpressionYeastMutationTwo tandem tet operators (2XtetO2) downstream of …PromoterPGal1-D12 promoterAvailable sinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFAB3893
Plasmid#47844PurposeBIOFAB RFP reporter plasmid for measuring promoter 13 + BCD1 efficiency.DepositorInsertpromoter 13 and BCD1
UseSynthetic BiologyTagsExpressionBacterialMutationPromotersee insertAvailable sinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMDC32B-OsMIR390-B/c
Plasmid#61467PurposePlant expression vector (2x35S) for direct cloning of artificial microRNAs into rice MIR390 precursor. To use for expressing amiRNAs in monocots.DepositorInsertOsMIR390-B/c
UseTagsExpressionPlantMutationO. sativa MIR390 precursor sequence including a c…Promoter2x35SAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCfB3039(XII-2 MarkerFree)
Plasmid#73279PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-2 (Chr XII: 808805..809939)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAcrF2
Plasmid#89234PurposePlasmid contains the gene for anti-CRISPR protein AcrF2 (gene 30 from bacteriophage D3112), with N-terminal 6his tag and TEV protease cleavage siteDepositorInsertAcrF2
UseTags6xHis-TEVExpressionMutationPromoterAvailable sinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCfB3036(XI-1 MarkerFree)
Plasmid#73274PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-1 (Chr XI: 67491..68573)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMDC123SB-OsMIR390-B/c
Plasmid#61466PurposePlant expression vector (2x35S) for direct cloning of artificial microRNAs into rice MIR390 precursor. To use for expressing amiRNAs in monocots.DepositorInsertOsMIR390-B/c
UseTagsExpressionPlantMutationO. sativa MIR390 precursor sequence including a c…Promoter2x35SAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDN-D2irG4kwh
Plasmid#44723DepositorInsertspCMV-D2i promoter
EGFP
UseSynthetic Biology; Expression regulator/reporterTagsRabbit β-globin intron II and WPREExpressionMammalianMutationInitiator motif (Inr) displaced relative to pCMV-…PromoterpCMV-D2iAvailable sinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAcrF1
Plasmid#89233PurposePlasmid contains the gene for anti-CRISPR protein AcrF1 (gene 35 from bacteriophage JBD30), with N-terminal 6his tag and TEV protease cleavage siteDepositorInsertAcrF1
UseTags6xHis-TEV and FLAGExpressionMutationPromoterAvailable sinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDN-G1Tt
Plasmid#44509DepositorInsertsUseSynthetic Biology; Expression regulator/reporterTagsExpressionYeastMutationTwo tandem tet operators (2XtetO2) downstream of …PromoterPGal1-D12 promoterAvailable sinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
PAX6 sgRNA2
Plasmid#68465Purposetargeting PAX6 geneDepositorInsertsgRNA targeting PAX6 (PAX6 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDN-T1GZmbh
Plasmid#44522DepositorInsertsUseSynthetic Biology; Expression regulator/reporterTagsExpressionYeastMutationThree tandem tet operators (3XtetO2) downstream o…PromoterPGal1-T123 promoterAvailable sinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFAB3992
Plasmid#47823PurposeBIOFAB RFP reporter plasmid for measuring promoter 14 + BCD16 efficiency.DepositorInsertpromoter 14 and BCD16
UseSynthetic BiologyTagsExpressionBacterialMutationPromotersee insertAvailable sinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFAB3725
Plasmid#47837PurposeBIOFAB RFP reporter plasmid for measuring promoter 6 + BCD1 efficiency.DepositorInsertpromoter 6 and BCD1
UseSynthetic BiologyTagsExpressionBacterialMutationPromotersee insertAvailable sinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFAB3869
Plasmid#47842PurposeBIOFAB RFP reporter plasmid for measuring promoter 11 + BCD1 efficiency.DepositorInsertpromoter 11 and BCD1
UseSynthetic BiologyTagsExpressionBacterialMutationPromotersee insertAvailable sinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCOCK CM-R03
Plasmid#46567PurposeMammalian expression plasmid with EGFP expressed from synthetic promoter (random mutagenesis of CMVwt 3' end).DepositorInsertCMV mutant promoter
UseSynthetic BiologyTagsExpressionMammalianMutationMutant version of CMV promoter (refer to Table 1 …PromoterAvailable sinceSept. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFAB1935
Plasmid#47860PurposeBIOFAB reporter plasmid for measuring his_T_min_linker_crp_T_min termination efficiencyDepositorInserthis_T_min_linker_crp_T_min double terminator
UseSynthetic BiologyTagsExpressionBacterialMutationconcatenate of his_min and crp_minPromoterpLTetO15Available sinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCOCK CMVwt
Plasmid#46559PurposeMammalian expression plasmid with EGFP expressed from CMV wild-type promoter.DepositorInsertCMV wt promoter
UseSynthetic BiologyTagsExpressionMammalianMutationOur version has a G at base 724 (reference has C)PromoterCMVAvailable sinceSept. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDN-G1GZmbh
Plasmid#44516DepositorInsertsUseSynthetic Biology; Expression regulator/reporterTagsExpressionYeastMutationTwo tandem tet operators (2XtetO2) downstream of …PromoterPGal1-D12 promoterAvailable sinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCOCK CM-R05
Plasmid#46568PurposeMammalian expression plasmid with EGFP expressed from synthetic promoter (random mutagenesis of CMVwt 3' end).DepositorInsertCMV mutant promoter
UseSynthetic BiologyTagsExpressionMammalianMutationMutant version of CMV promoter (refer to Table 1 …PromoterAvailable sinceSept. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFAB3913
Plasmid#47816PurposeBIOFAB RFP reporter plasmid for measuring promoter 14 + BCD9 efficiency.DepositorInsertpromoter 14 and BCD9
UseSynthetic BiologyTagsExpressionBacterialMutationPromotersee insertAvailable sinceAug. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCOCK CM-C02
Plasmid#46563PurposeMammalian expression plasmid with EGFP expressed from synthetic promoter (CMVwt CAAT box mutant).DepositorInsertCMV mutant promoter
UseSynthetic BiologyTagsExpressionMammalianMutationMutant version of CMV promoter (refer to Table 1 …PromoterAvailable sinceSept. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCOCK CM-C18
Plasmid#46560PurposeMammalian expression plasmid with EGFP expressed from synthetic promoter (CMVwt CAAT box mutant).DepositorInsertCMV mutant promoter
UseSynthetic BiologyTagsExpressionMammalianMutationMutant version of CMV promoter (refer to Table 1 …PromoterAvailable sinceSept. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCfB3038(XII-1 MarkerFree)
Plasmid#73278PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-1 (Chr XII: 795787..796720)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB3040(XII-4 MarkerFree)
Plasmid#73280PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-4 (Chr XII:830227..831248)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGL2-HKII-D
Plasmid#64516PurposeAS-30D hepatoma hexokinase II 1.0 kbp promoter-luciferase reporter vectorDepositorInsertType II hexokinase gene, partial cds and promoter region (Hk2 Rat)
UseLuciferaseTagsfirefly luciferaseExpressionMutationIsolated from AS-30D rat hepatomaPromoterHexokinase II promoterAvailable sinceJune 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCfB3037(XI-5 MarkerFree)
Plasmid#73277PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-5 (Chr XI: 11779..118967)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFAB3665
Plasmid#47832PurposeBIOFAB RFP reporter plasmid for measuring promoter 1 + BCD1 efficiency.DepositorInsertpromoter 1 and BCD1
UseSynthetic BiologyTagsExpressionBacterialMutationPromotersee insertAvailable sinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFAB3701
Plasmid#47835PurposeBIOFAB RFP reporter plasmid for measuring promoter 4 + BCD1 efficiency.DepositorInsertpromoter 4 and BCD1
UseSynthetic BiologyTagsExpressionBacterialMutationPromotersee insertAvailable sinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFAB3845
Plasmid#47840PurposeBIOFAB RFP reporter plasmid for measuring promoter 9 + BCD1 efficiency.DepositorInsertpromoter 9 and BCD1
UseSynthetic BiologyTagsExpressionBacterialMutationPromotersee insertAvailable sinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFAB3857
Plasmid#47841PurposeBIOFAB RFP reporter plasmid for measuring promoter 10 + BCD1 efficiency.DepositorInsertpromoter 10 and BCD1
UseSynthetic BiologyTagsExpressionBacterialMutationPromotersee insertAvailable sinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFAB1902
Plasmid#47858PurposeBIOFAB reporter plasmid for measuring M13_central_T_linker_rrnD_T1 termination efficiencyDepositorInsertM13_central_T_linker_rrnD_T1 double terminator
UseSynthetic BiologyTagsExpressionBacterialMutationconcatenate of M13 central and rrnDPromoterpLTetO13Available sinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFAB3997
Plasmid#47828PurposeBIOFAB RFP reporter plasmid for measuring promoter 14 + BCD21 efficiency.DepositorInsertpromoter 14 and BCD21
UseSynthetic BiologyTagsExpressionBacterialMutationPromotersee insertAvailable sinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFAB3912
Plasmid#47815PurposeBIOFAB RFP reporter plasmid for measuring promoter 14 + BCD8 efficiency.DepositorInsertpromoter 14 and BCD8
UseSynthetic BiologyTagsExpressionBacterialMutationPromotersee insertAvailable sinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-mMyh1CDS
Plasmid#192839PurposePlasmid to produce ISH probe for mMyh1 gene with CDS sequenceDepositorInsertmyosin heavy chain 1 (Myh1 Mouse)
UseTagsExpressionMutationPromoterAvailable sinceDec. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-mMyh1UTR
Plasmid#192840PurposePlasmid to produce ISH probe for mMyh1 gene with UTR sequenceDepositorInsertmyosin heavy chain 1 (Myh1 Mouse)
UseTagsExpressionMutationPromoterAvailable sinceDec. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRGEB32-CrActin-35S
Plasmid#226708PurposeFor CRISPR/Cas-mediated gene editing in model fern species, Ceratopteris richardii; expresses guide RNAs from CrActin promoter and Cas9 from CaMV 35S promoter.DepositorInsertCrActin
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRGEB32-CrActin-UBI
Plasmid#226709PurposeFor CRISPR/Cas-mediated gene editing in model fern species, Ceratopteris richardii; expresses guide RNAs from CrActin promoter and Cas9 from maize ubiquitin (ZmUbi) promoter.DepositorInsertCrActin
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEL639
Plasmid#189833PurposeThe T-DNA backbone of Cas12j2 for DNA methylation-based gene silencing in riceDepositorTypeEmpty backboneUseCRISPR; T-dna cloneTagsExpressionMutationPromoterZmUbi1Available sinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX330_SAFB2gRNA12
Plasmid#196106PurposeContains guide RNA to 3' end of mouse SAFB2 gene for safb1/2 dko. Used with Addgene IDs: 196103, 196107, 196108DepositorInsertSAFB2 (Safb2 Mouse)
UseCRISPR and Mouse TargetingTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX330_SAFB2gRNA13
Plasmid#196107PurposeContains guide RNA to 3' end of mouse SAFB2 gene for safb1/2 dko. Used with Addgene IDs: 196103, 196106, 196108DepositorInsertSAFB2 (Safb2 Mouse)
UseCRISPR and Mouse TargetingTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCA22-SEC61B 5'HA-TriTag (mTagBFP)-3'HA (HDR donor)
Plasmid#199453PurposeCRISPR donor plasmid to insert TriTag (mTagBFP) into the C-terminus of human SEC61B geneDepositorInsertHDR donor template
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGreenII 0229/ProRUBY:GOXL6-Citrine:NOSt
Plasmid#175568PurposepSOUP helper plasmid needed for Agrobacterium-mediated plant transformation - Expression of GOXL genes tagged with Citrine under RUBY promoter for transgene complementation of ruby-6DepositorInsertGOXL6
UseTagsCitrineExpressionPlantMutationPromoterRUBYAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGreenII 0229/ProRUBY:GOXL5-Citrine:NOSt
Plasmid#175567PurposepSOUP helper plasmid needed for Agrobacterium-mediated plant transformation - Expression of GOXL genes tagged with Citrine under RUBY promoter for transgene complementation of ruby-5DepositorInsertGOXL5
UseTagsCitrineExpressionPlantMutationPromoterRUBYAvailable sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGreenII 0229/ProRUBY:GOXL4-Citrine:NOSt
Plasmid#175566PurposepSOUP helper plasmid needed for Agrobacterium-mediated plant transformation - Expression of GOXL genes tagged with Citrine under RUBY promoter for transgene complementation of ruby-4DepositorInsertGOXL4
UseTagsCitrineExpressionPlantMutationPromoterRUBYAvailable sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only