We narrowed to 2,123 results for: PAM

1. pXD70Tet(DadH)-Pdadh-DadhABC(A2)

   Plasmid

   #191644

   Purpose

   E. coli - Eggerthella lenta shuttle plasmid (TetR), native promoter-dopamine dehydroxylase from dopamine-metabolizing E. lenta A2 strain

   Depositor

   Emily Balskus

   Article

   Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

   Insert

   Dopamine dehydroxylase
   

   Expression

   Bacterial

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. MBP_Lims (MBP_LIM1234)

   Plasmid

   #182042

   Purpose

   For expression of human paxillin LIM domains 1-4 with N-terminal GST fusion protein in E. coli.

   Depositor

   Vesa Hytönen

   Article

   Ripamonti et al Commun Biol. 2021 Mar 29;4(1):380. doi:

   Insert

   LIM1234 (PXN Human)
   

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   LIM domains 1-4 only

   Available Since

   April 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. GST_LIM234

   Plasmid

   #182039

   Purpose

   For expression of human paxillin LIM domains 2-4 with N-terminal GST fusion protein in E. coli.

   Depositor

   Vesa Hytönen

   Article

   Ripamonti et al Commun Biol. 2021 Mar 29;4(1):380. doi:

   Insert

   LIM234 (PXN Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   LIM domains 2-4 only

   Available Since

   April 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hSynapsin1-RdLight1

   Plasmid

   #125708

   Purpose

   Expresses the red genetically encoded dopamine sensor RdLight1 in mammalian neurons.

   Depositor

   Lin Tian

   Article

   Patriarchi et al Nat Methods. 2020 Nov;17(11):1147-1155.

   Has Service

   AAV9

   Insert

   RdLight1
   

   Use

   AAV

   Tags

   Flag tag

   Promoter

   hSynapsin1

   Available Since

   Aug. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-CAG-RdLight1

   Plasmid

   #125707

   Purpose

   Expresses the red genetically encoded dopamine sensor RdLight1 in mammalian cells.

   Depositor

   Lin Tian

   Article

   Patriarchi et al Nat Methods. 2020 Nov;17(11):1147-1155.

   Has Service

   AAV9

   Insert

   dLight1.3b
   

   Use

   AAV

   Tags

   Flag tag

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. MTOR-F2108L_CD19-CAR-28z-2A-EGFP_AAV6_Donor

   Plasmid

   #211904

   Purpose

   Vector for AAV6 donor production. Targeted CD19-CAR-28z-2A-EGFP transgene integration to the MTOR locus with the dominant rapamycin resistance MTOR-F2108L mutation via homology-directed repair.

   Depositor

   Yannick Doyon

   Article

   Levesque et al bioRxiv 2023.09.14.557485

   Insert

   CD19-CAR-28z-2A-EGFP
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   hPGK1

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-CBX1mut-PA-mCherry

   Plasmid

   #138251

   Purpose

   Triple-mutant CBX1 chromodomain (residues 20-73) tagged with photoactivatable mCherry (PAmCherry). Mutations V3E/K6E/D40S correspond to positions V22/K25/D59 in full-length CBX1.

   Depositor

   Jin Zhang

   Article

   Hard et al Sci Adv. 2018 Nov 7;4(11):eaau1447. doi:

   Insert

   CBX1mut-PAmCherry
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-LGC-53

   Plasmid

   #86399

   Purpose

   Mammalian expression of codon-optimized dopamine receptor LGC-53

   Depositor

   Steven Mennerick

   Article

   Kress et al J Neurosci. 2014 Aug 27;34(35):11792-802

   Insert

   LGC-53 (lgc-53 Nematode, Synthetic)
   

   Expression

   Mammalian

   Mutation

   Codon optimized for mammalian expression; Signal …

   Promoter

   CMV

   Available Since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. MTOR-F2108L_DARIC(1)_CD19-scFv_AAV6_Donor

   Plasmid

   #211905

   Purpose

   Vector for AAV6 donor production. Targeted CD19-scFv DARIC transgene integration to the MTOR locus with the dominant rapamycin resistance MTOR-F2108L mutation via homology-directed repair.

   Depositor

   Yannick Doyon

   Article

   Levesque et al bioRxiv 2023.09.14.557485

   Insert

   DARIC-CD19-scFv
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   hPGK1

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pXD70DA7-DadR/DadS-Pdadh-DadhABC(A2)

    Plasmid

    #191630

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (KanR), native promoter-dopamine dehydroxylase from dopamine-metabolizing E. lenta A2 strain with transcriptional regulators DadR/DadS

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    Dopamine dehydroxylase
    

    Expression

    Bacterial

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pXD70Tet(DadHR)-DadR-Pdadh-DadhABC(A2)

    Plasmid

    #191645

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (TetR), native promoter-dopamine dehydroxylase from dopamine-metabolizing E. lenta A2 strain with transcriptional regulator DadR

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    Dopamine dehydroxylase
    

    Expression

    Bacterial

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-syn-dLight1.3b

    Plasmid

    #135762

    Purpose

    For expression of the genetically encoded dopamine sensor dLight1.3b

    Depositor

    Lin Tian

    Article

    Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

    Has Service

    AAV9

    Insert

    dLight1.3b
    

    Use

    AAV

    Tags

    Flag tag

    Expression

    Mammalian

    Promoter

    Synapsin

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGMβ1

    Plasmid

    #216202

    Purpose

    Chromosomal gene manipulation (gene insertion, conversion, deletion) of dairy used Lactobacillus bulgaricus, a difficult gene to manipulate, is now possible by conjugation using this pGMB1 plasmid.

    Depositor

    Yasuko Sasaki

    Article

    Iwamoto et al Biosci Microbiota Food Health. 2022;41(1

    Type

    Empty backbone

    Use

    Shuttle vector, conjugal plasmid, theta type rep…

    Expression

    Bacterial

    Promoter

    lac promoter in pGEM-Teasy,　Promoters in pAMbeta1…

    Available Since

    Jan. 21, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pCDNA5_FRB-pBirA-HA

    Plasmid

    #83415

    Purpose

    proximity-directed biotin labeling on the FRB domain of mTOR in mammalian cell

    Depositor

    Hyun-Woo Rhee

    Article

    Lee et al ACS Cent Sci. 2016 Aug 24;2(8):506-16. d

    Insert

    FRB-pBirA-HA (MTOR Human, Synthetic)
    

    Tags

    HA and pBirA

    Expression

    Mammalian

    Promoter

    CMV-F

    Available Since

    March 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pXD70DAmt1-DadR/DadS-Pdadh-DadhABC(R506S, A2)

    Plasmid

    #191631

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (KanR), native promoter-dopamine dehydroxylase from dopamine-metabolizing E. lenta A2 strain (R506S mutation) with transcriptional regulators DadR/DadS

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    Dopamine dehydroxylase (R506S mutation, inactive form)
    

    Expression

    Bacterial

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-CAG-dLight1.3b

    Plasmid

    #125560

    Purpose

    For expression of the genetically encoded dopamine sensor dLight1.3b

    Depositor

    Lin Tian

    Article

    Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

    Has Service

    AAV1, AAV5, and AAV9

    Insert

    dLight1.3b
    

    Use

    AAV

    Tags

    Flag tag

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-hSyn-GRAB_DA1m

    Plasmid

    #113049

    Purpose

    express the genetically-encoded fluorescent dopamine(DA) sensor GRAB_DA1m in neurons

    Depositor

    Yulong Li

    Article

    Sun et al Cell. 2018 Jul 12;174(2):481-496.e19. do

    Has Service

    AAV9

    Insert

    GPCR activation based DA sensor GRAB_DA1m (DRD2 Human)
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-hSyn-GRAB_DA1h

    Plasmid

    #113050

    Purpose

    express the genetically-encoded fluorescent dopamine(DA) sensor GRAB_DA1h in neurons

    Depositor

    Yulong Li

    Article

    Sun et al Cell. 2018 Jul 12;174(2):481-496.e19. do

    Has Service

    AAV Retrograde and AAV9

    Insert

    GPCR activation based DA sensor GRAB_DA1h (DRD2 Human)
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. Phage-PGK-Flag-HA-FKBP-T(WT)

    Plasmid

    #181734

    Purpose

    for lentiviral expression of brachyury ORF in mammalian cells

    Depositor

    Charles Lin

    Article

    Sheppard et al Cell Rep Med. 2021 Jan 19;2(1):100188. d

    Insert

    T (brachyury)
    

    Use

    Lentiviral

    Mutation

    a silent mutation in the PAM site of an sgRNAt ta…

    Available Since

    March 21, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. peSCBE3-NG-HF1-Hypa

    Plasmid

    #218166

    Purpose

    This plasmid harbors the base editor eSCBE3-NG-HF1-Hypa along with an sgRNA cloning cassette, facilitating high-efficiency and high-fidelity base editing at targets bearing NG PAM in Streptomyces.

    Depositor

    Yuhui Sun

    Article

    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

    Inserts

    escbe3-NG-HF1-Hypa
    a programmable sgRNA cloning cassette
    

    Use

    CRISPR; Sgrna transcription

    Expression

    Bacterial

    Mutation

    Mutation in the portion of deaminase hAPOBEC3A : …

    Promoter

    rpsL promoter

    Available Since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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