Addgene

Showing: 1041 - 1060 of 5765 results

Results per page:

Sort results by:

pMRX-IP/SECFP-mATG5

Plasmid

#58744

Purpose

Expresses /SECFP-ATG5 in mammalian cells

Depositor

Noboru Mizushima

Article

Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

Insert

autophagy related genes 5 (Atg5 Mouse)

Use

Retroviral

Tags

SECFP

Expression

Mutation

Promoter

Available since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only
pMKO.1-shEsco2

Plasmid

#160957

Purpose

Esco2 shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Esco2 shRNA (Esco2 Mouse)

Use

RNAi and Retroviral

Tags

Expression

Mutation

Promoter

U6

Available since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-FLEX-SaCas9-U6-sgGabrg3

Plasmid

#124858

Purpose

Mutagenesis of Gabrg3

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Gabrg3 (Gabrg3 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-FLEXfrt-SaCas9-U6-sgGabrg3

Plasmid

#124872

Purpose

Mutagenesis of Gabrg3

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Gabrg3 (Gabrg3 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-EF1a-V5-Arl6ip6

Plasmid

#120235

Purpose

Expresses V5-tagged Arl6ip6 in lentiviral vector

Depositor

Larry Gerace

Article

Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

Insert

Arl6ip6 (Arl6ip6 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mutation

Promoter

EF1a

Available since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMX_hTAF4_Cterminus

Plasmid

#44335

Depositor

Marc Timmers

Article

Pijnappel et al Nature. 2013 Mar 28;495(7442):516-9. doi

Insert

TAF4 (TAF4 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Functional C-terminal fragment 485-1085(end)

Promoter

LTR

Available since

May 14, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX SH2D4B-SH2

Plasmid

#46482

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

SH2D4B (Sh2d4b Mouse)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 311-431

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pBluescript-Maf

Plasmid

#98179

Purpose

Expression of a cMaf in situ hybridization probe

Depositor

Oscar Marin

Article

Lim et al Nat Neurosci. 2018 Jun 18. pii: 10.1038/

Insert

cMaf, partial mRNA (Maf Mouse)

Use

In situ hybridization

Tags

Expression

Mutation

Promoter

T3/T7

Available since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pET32b-MED19

Plasmid

#15438

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

MED19 (Med19 Mouse)

Use

Tags

His and Trx

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
pET41a-MED30

Plasmid

#15431

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

MED30 (Med30 Mouse)

Use

Tags

GST and His

Expression

Bacterial

Mutation

N/A

Promoter

Available since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
CIP2Aprom27bp-pGL4.10Luc

Plasmid

#60876

Purpose

Luc reporter driven by 27 bp of the CIP2A promoter

Depositor

Jukka Westermarck

Article

Khanna et al PLoS One. 2011 Mar 22;6(3):e17979. doi:

Insert

27 bp promoter fragment of Cancerous Inhibitor of PP2A (CIP2A Human)

Use

Luciferase; Promoterless

Tags

Expression

Mutation

Promoter

Available since

April 5, 2016

Availability

Academic Institutions and Nonprofits only
myc-Med19 pBacPAK8

Plasmid

#15376

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

Med19 (Med19 Mouse)

Use

Baculovirus construction

Tags

myc

Expression

Mutation

Promoter

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pTag4C_Nrg1-GV-2HA

Plasmid

#227104

Purpose

Nrg1 protein for luciferase and barcode assays

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

Nrg1-GV (Nrg1 Mouse)

Use

Tags

2xHA

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
TMEM16F-I521A-peGFP-N1

Plasmid

#228577

Purpose

Expresses mouse TMEM16F-I521A-eGFP in mammalian cells

Depositor

Huanghe Yang

Article

Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

Insert

TMEM16F/anoctamin 6 (Ano6), transcript variant 2 (Ano6 Mouse)

Use

Tags

Expression

Mammalian

Mutation

I521A plus a 3 amino acid N-terminal truncation (…

Promoter

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
TMEM16F-I521E-peGFP-N1

Plasmid

#228578

Purpose

Expresses mouse TMEM16F-I521E-eGFP in mammalian cells

Depositor

Huanghe Yang

Article

Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

Insert

TMEM16F/anoctamin 6 (Ano6), transcript variant 2 (Ano6 Mouse)

Use

Tags

Expression

Mammalian

Mutation

I521E plus a 3 amino acid N-terminal truncation (…

Promoter

Available since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 TREK-2(S156C)

Plasmid

#224770

Purpose

In vitro transcription for Oocyte expression of 1 TREK-2(S156C)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily member 10 (Kcnk10 Mouse)

Use

In vitro transcription for oocyte expression

Tags

Expression

Mutation

S156C

Promoter

T7

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pPB-cT3G-cERP2-SOX17

Plasmid

#222925

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of SOX17

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

SOX17 (SOX17 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-IRAK4-mScarlet-P2A-Puro

Plasmid

#221237

Purpose

Stable expression of mouse IRAK4-mScarlet after Lentiviral transduction

Depositor

Jonathan Kagan

Article

Fisch et al Nature. 2024 Jul;631(8021):635-644. doi:

Insert

IRAK4 (Irak4 Mouse)

Use

Lentiviral

Tags

mScarlet

Expression

Mutation

Promoter

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRISPR-cBEST-v2-SF14P

Plasmid

#209448

Purpose

improved pCRISPR-cBEST plasmid with optimized sgRNA cloning site and expression of the sgRNA from the SF14P promoter

Depositor

Tilmann Weber

Article

Maleckis et al ACS Chem Biol. 2024 Feb 10. doi: 10.1021

Insert

Codon optimized APOBEC1-nCas9-UGI fusion protein

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

PtipA; sgRNA expression from SF14P promoter

Available since

March 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRISPR-cBEST-v2-kasOP*

Plasmid

#209447

Purpose

improved pCRISPR-cBEST plasmid with optimized sgRNA cloning site and expression of the sgRNA from the kasOP* promoter

Depositor

Tilmann Weber

Article

Maleckis et al ACS Chem Biol. 2024 Feb 10. doi: 10.1021

Insert

Codon optimized APOBEC1-nCas9-UGI fusion protein

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

PtipA; sgRNA expression from kasOP* promoter

Available since

March 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge