We narrowed to 7,347 results for: alp;

1. pBabe-GFP-IKBalpha-mut (super repressor)

   Plasmid

   #15264

   Depositor

   William Hahn

   Article

   Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

   Insert

   IKB alpha (NFKBIA Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Serine 32 to Alanine, Serine 36 to Alanine

   Promoter

   Available since

   July 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBabe-Puro-IKBalpha-mut (super repressor)

   Plasmid

   #15291

   Depositor

   William Hahn

   Article

   Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

   Insert

   inhibitor of Kappa B alpha (NFKBIA Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   S32A/S36A: “super-repressor” allele (resistant to…

   Promoter

   Available since

   July 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pU6-sgRNA EF1Alpha-puro-T2A-BFP.CYRANO

   Plasmid

   #128755

   Purpose

   Expresses CYRANO gRNA for CRISPRi

   Depositor

   David Bartel

   Article

   Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

   Insert

   CYRANO sgRNA (OIP5-AS1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mU6

   Available since

   Aug. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. PGC-1 alpha promoter luciferase delta CRE

   Plasmid

   #8888

   Depositor

   Bruce Spiegelman

   Article

   Handschin et al Proc Natl Acad Sci U S A. 2003 Jun 10. 1

   Insert

   PGC-1 alpha promoter dCRE (Ppargc1a Mouse)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Site-directed mutagenesis to remove the CREB bind…

   Promoter

   Available since

   Feb. 13, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. Anti-Slo1/BKAlpha maxi-K+ channel [L6/60R]

   Plasmid

   #177486

   Purpose

   Mammalian Expression Plasmid of anti-Slo1/BKAlpha maxi-K+ channel (Mouse). Derived from hybridoma L6/60.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   anti-Slo1/BKAlpha maxi-K+ channel (Mus musculus) recombinant mouse monoclonal antibody (Kcnma1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Dual CMV

   Available since

   Nov. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLVX-EF1alpha-nCoV2019-N-meGFP-IRES-Puro

   Plasmid

   #185451

   Purpose

   Expresses SARS-CoV-2 nucleocapsid protein with a c-terminal monomeric EGFP in mammalian cells

   Depositor

   Ahmet Yildiz

   Article

   Jack et al PLoS Biol. 2021 Oct 11;19(10):e3001425.

   Insert

   Nucleocapsid (N SARS-CoV-2)
   

   Use

   Lentiviral

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLVX-CMV-Galpha-s-IRESWT-Gbetagamma ONE-GO

   Plasmid

   #204340

   Purpose

   GPCR/G protein BRET biosensor

   Depositor

   Mikel Garcia-Marcos

   Article

   Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

   Insert

   Galpha-s/Gbetagamma ONE-GO
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 19, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. AAV-FLEX-PKIalpha-IRES-nls-mRuby2

   Plasmid

   #63059

   Purpose

   Expresses PKIalpha and nuclear localized mRuby2 (linked with IRES) in a Cre-dependent manner in an AAV backbone

   Depositor

   Bernardo Sabatini

   Article

   Chen et al Front Pharmacol. 2014 Apr 2;5:56. doi: 1

   Insert

   PKIalpha (Pkia Synthetic, Mouse)
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. PGC-1 alpha promoter luciferase delta MEF

   Plasmid

   #8889

   Depositor

   Bruce Spiegelman

   Article

   Handschin et al Proc Natl Acad Sci U S A. 2003 Jun 10. 1

   Insert

   PGC-1 alpha promoter dMEF (Ppargc1a Mouse)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Site-directed mutagenesis to remove the MEF2 bind…

   Promoter

   Available since

   Feb. 13, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLenti-EF1alpha-IRES-EGFP-3XFlag-hCEP164

    Plasmid

    #122966

    Purpose

    Expresses 3XFlag-tagged human CEP164 in mammalian cells

    Depositor

    FENG-QIAN Li, Ken-Ichi Takemaru

    Insert

    CEP164 (CEP164 Human)
    

    Use

    Lentiviral

    Tags

    3XFlag

    Expression

    Mutation

    Promoter

    EF1 alpha

    Available since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDGB3_alpha1-pAtUBQ10:HA-LRR-NEL-mcherry-Tnos

    Plasmid

    #211840

    Purpose

    Plant transient expression cassette for the catalytically inactive E3-DART ligase under a UBQ10 promoter.

    Depositor

    Marcela Rojas-Pierce

    Article

    Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

    Insert

    LRR-NEL
    

    Use

    Synthetic Biology

    Tags

    HA and mCherry

    Expression

    Plant

    Mutation

    Promoter

    UBIQUITIN10

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDGB3_alpha2_4xCBS-mDFR-Rep-RepA-tNOS (GB3625)

    Plasmid

    #225433

    Purpose

    Transcriptional unit for copper-regulation of BeYDV Rep/RepA with 4xCBS domain+minimal promoter DFR for induction by copper.

    Depositor

    Diego Orzaez

    Insert

    4xCBS:mDFR:Rep/RepA:Tnos
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. V309 - pDNR MCS SA lacZ alpha - Donor

    Plasmid

    #11692

    Depositor

    Tony Pawson

    Article

    Colwill et al BMC Biotechnol. 2006 . 6():13.

    Type

    Empty backbone

    Use

    Cre/Lox; Donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 24, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. hum alpha ENAC promoter a25-a23

    Plasmid

    #83427

    Purpose

    promoter reporter construct to test 5' flanking sequences

    Depositor

    Christie Thomas

    Article

    Sayegh et al J Biol Chem. 1999 Apr 30;274(18):12431-7

    Insert

    human aENaC gene 5' flanking seq + 55 nt of the 5' UTR for exon 1A (SCNN1A Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mutation

    Promoter

    alpha ENAC

    Available since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. Stat1 alpha Y701F Flag pRc/CMV

    Plasmid

    #8702

    Depositor

    Jim Darnell

    Article

    Wen et al Cell 1995 Jul 28;82(2):241-50.

    Insert

    STAT1 alpha Y701F (STAT1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Y701F

    Promoter

    Available since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

16. pDGB3_alpha1-pAtUBQ10:HA-LRR-NELC492A-mcherry-Tnos

    Plasmid

    #211843

    Purpose

    Plant transient expression cassette for the E3-DART ligase under a UBQ10 promoter.

    Depositor

    Marcela Rojas-Pierce

    Article

    Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

    Insert

    LRR-NEL
    

    Use

    Synthetic Biology

    Tags

    HA and mCherry

    Expression

    Plant

    Mutation

    C492A

    Promoter

    UBIQUITIN10

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PBS/pU6- HIF1 alpha RNAi plasmid 1

    Plasmid

    #21103

    Depositor

    Connie Cepko

    Article

    Chen et al Science. 2009 Jan 9. 323(5911):256-9.

    Insert

    RNAi against hypoxia inducible factor 1alpha
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLVX-hSyn-Galpha-i3-IRESWT-Gbetagamma ONE-GO

    Plasmid

    #204343

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Insert

    hSyn Galpha-i3/Gbetagamma ONE-GO
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pDGB3_alpha1-AtUBQ10:HR1bR181/185A-GFP-T35S

    Plasmid

    #211839

    Purpose

    Plant transient expression cassette for GFP fused to the mutant HR1b degron sequence under UBQ10 promoter.

    Depositor

    Marcela Rojas-Pierce

    Article

    Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

    Insert

    HR1b
    

    Use

    Synthetic Biology

    Tags

    GFP

    Expression

    Plant

    Mutation

    R181A R185A

    Promoter

    UBIQUITIN10

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pU6-sgRNA EF1Alpha-puro-T2A-BFP.Control

    Plasmid

    #128749

    Purpose

    Expresses control gRNA

    Depositor

    David Bartel

    Article

    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

    Insert

    Control sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge