We narrowed to 146,057 results for: ins;

1. pDD282

   Plasmid

   #66823

   Purpose

   GFP^SEC^3xFlag vector with ccdB markers for cloning homology arms

   Depositor

   Bob Goldstein

   Article

   Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

   Insert

   GFP-C1^SEC^3xFlag
   

   Use

   CRISPR and Cre/Lox

   Tags

   3xFlag and C. elegans codon-optimized GFP

   Expression

   Worm

   Mutation

   Promoter

   Available since

   June 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pUASP dsRed Rab7 WT

   Plasmid

   #44824

   Depositor

   Matthew Scott

   Article

   Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

   Insert

   Rab7 (Rab7 Fly)
   

   Use

   Drosophila

   Tags

   dsRed

   Expression

   Insect

   Mutation

   Promoter

   Available since

   April 18, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pN7CAST

   Plasmid

   #190661

   Purpose

   Expresses polycistronic N7 CAST where Cas12k-T2A-TnsC and TniQ-T2A-TnsB are separated by an EMCV IRES

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   human codon optimized N7Cas12k, N7TnsC, N7TniQ, N7TnsB
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDonor14-AcHELIX-KanR (CJT96)

   Plasmid

   #181794

   Purpose

   pDonor for AcHELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequence and encoding a kanamycin resistance gene as cargo

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   KanR, R6K origin of replication
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pFA6a-mCherry-sfGFP-kanMX (pMaM17)

   Plasmid

   #173452

   Purpose

   Contains mCherry-sfGFP tandem fluorescent protein timer

   Depositor

   Anton Khmelinskii

   Article

   Khmelinskii et al Nat Biotechnol. 2012 Jun 24;30(7):708-14

   Insert

   mCherry-sfGFP-ADH1ter
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   no promoter

   Available since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMW37: pRS426-R2Bm RNA

   Plasmid

   #209291

   Purpose

   Contains the R2Bm non-LTR retrotransposon 5'UTR and 3'UTR sequences

   Depositor

   Feng Zhang

   Article

   Wilkinson et al Science. 2023 Apr 21;380(6642):301-308.

   Insert

   R2Bm 5'UTR and 3'UTR
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   GAL1 GAPDH

   Available since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDD283

   Plasmid

   #66824

   Purpose

   YPET^SEC^3xFlag vector with ccdB markers for cloning homology arms

   Depositor

   Bob Goldstein

   Article

   Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

   Insert

   YPET-C1^SEC^3xFlag
   

   Use

   CRISPR and Cre/Lox

   Tags

   3xFlag and C. elegans codon-optimized YPET

   Expression

   Worm

   Mutation

   Promoter

   Available since

   June 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDonor14-ShoHELIX-KanR (BO4)

   Plasmid

   #181795

   Purpose

   pDonor for ShoHELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequence and encoding a kanamycin resistance gene as cargo

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   KanR, R6K origin of replication
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pUASp YFP Rab5 WT

   Plasmid

   #53468

   Depositor

   Matthew Scott

   Article

   Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

   Insert

   Rab05 (Rab5 Fly)
   

   Use

   Tags

   YFP

   Expression

   Insect

   Mutation

   Promoter

   GAL4

   Available since

   June 10, 2014

   Availability

   Academic Institutions and Nonprofits only

10. CIRTS-10: bdefensin 3-SLBP-YTHDF2(1-200)

    Plasmid

    #132546

    Purpose

    Expresses CIRTS-10 (bdefensin 3-SLBP-YTHDF2(1-200)) in mammalian cells

    Depositor

    Bryan Dickinson

    Article

    Rauch et al Cell. 2019 Jun 27;178(1):122-134.e12. do

    Insert

    CIRTS-10: bdefensin 3-SLBP-YTHDF2(1-200)
    

    Use

    Tags

    FLAG tag

    Expression

    Mammalian

    Mutation

    Promoter

    cmv d0

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. SLBP non-targeting gRNA lambda phage

    Plasmid

    #132548

    Purpose

    expresses gRNA for SLBP-based CIRTS

    Depositor

    Bryan Dickinson

    Article

    Rauch et al Cell. 2019 Jun 27;178(1):122-134.e12. do

    Insert

    gRNA SLBP-based CIRTS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6 promoter

    Available since

    Sept. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAcCAST-sgRNA_entry (CJT83)

    Plasmid

    #181785

    Purpose

    Expresses AcCAST. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    AcTnsB, AcTnsC, AcTniQ, AcCas12k
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Lac and J23119

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pShoCAST-sgRNA_entry (BO1)

    Plasmid

    #181786

    Purpose

    Expresses ShoCAST. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    ShoTnsB, ShoTnsC, ShoTniQ, ShoCas12k
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Lac and J23119

    Available since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Cmv d0 dPspCas13b-GGS-NYTHDF1(100-200)

    Plasmid

    #119857

    Purpose

    Cmv d0 dPspCas13b-GGS-NYTHDF1(100-200) (YTHDF1 100-200 subdomain)

    Depositor

    Bryan Dickinson

    Article

    Rauch et al J Am Chem Soc. 2018 Sep 26;140(38):11974

    Insert

    dCas13b-GGS-NYTHDF1(100-200)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Cmv d0 dPspCas13b-GGS-NYTHDF2(100-200)

    Plasmid

    #119856

    Purpose

    Cmv d0 dPspCas13b-GGS-NYTHDF2(100-200) (YTDHF2 100-200 subdomain)

    Depositor

    Bryan Dickinson

    Article

    Rauch et al J Am Chem Soc. 2018 Sep 26;140(38):11974

    Insert

    dCas13b-GGS-NYTHDF2(100-200)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. IBMc460

    Plasmid

    #161940

    Purpose

    Carries Golden Gate substitution insert for IBM with gp41-1 intein. PPhlF as C-lobe promoter

    Depositor

    Baojun Wang

    Article

    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

    Insert

    pSB1A3-BbsI-GY-gp-41-1N-cat-PPhlF-RiboJ-B32-gp41-1C-SS-SapI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PPhlF

    Available since

    March 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCEP4 Smad2

    Plasmid

    #16484

    Depositor

    Bert Vogelstein

    Article

    Riggins et al Nat Genet. 1996 Jul . 13(3):347-9.

    Insert

    Smad2 (SMAD2 Human)
    

    Use

    Tags

    Double HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

18. CMV500 A-SREBP1

    Plasmid

    #33357

    Depositor

    Charles Vinson

    Article

    Rishi et al J Biol Chem. 2004 Mar 19;279(12):11863-7

    Insert

    SREBP1 (Srebf1 Cricetulus griseus (hamster))
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Dominant Negative (see notes)

    Promoter

    CMV

    Available since

    Dec. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pENTR-Arl13b1

    Plasmid

    #40870

    Depositor

    Tamara Caspary

    Article

    Larkins et al Mol Biol Cell. 2011 Dec;22(23):4694-703.

    Insert

    Arl13b (Arl13b Mouse)
    

    Use

    Gateway entry

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

20. IBMc233

    Plasmid

    #161939

    Purpose

    Carries Golden Gate substitution insert for IBM with M86 intein. PPhlF as C-lobe promoter

    Depositor

    Baojun Wang

    Article

    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

    Insert

    pSB1A3-BbsI-M86N-T-cat-PPhlF-RiboJ-B32-M86C-SapI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PPhlF

    Available since

    March 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge