We narrowed to 1,621 results for: nif

1. pPAGFP-C1

   Plasmid

   #11910

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Patterson et al Science. 2002 Sep 13. 297(5588):1873-7.

   Has Service

   Cloning Grade DNA

   Insert

   PAGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   These plasmids are essentially the same as the Cl…

   Promoter

   Available since

   July 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAT001

   Plasmid

   #171061

   Purpose

   Plasmid for expressing HIV-1 envelope glycoprotein

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

   Insert

   HIV-1 Env
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. IL6-GFP

   Plasmid

   #28088

   Depositor

   Jennifer Stow

   Article

   Manderson et al J Cell Biol. 2007 Jul 2. 178(1):57-69.

   Insert

   IL6 (Il6 Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. JunB

   Plasmid

   #187903

   Purpose

   Expresses JunB in transfected cells

   Depositor

   Jennifer Mitchell

   Article

   Mitchell et al Endocrinology. 2005 Apr;146(4):2048-54.

   Insert

   JunB (Junb Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPP089

   Plasmid

   #158796

   Purpose

   pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-3 in MCS1 for expression in E. coli and protein purification

   Depositor

   Jennifer Doudna

   Article

   Pausch et al Science. 2020 Jul 17;369(6501):333-337.

   Insert

   CasPhi-3
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDAC569

   Plasmid

   #195241

   Purpose

   Mammalian expression of Csm complex (RNase mut) from separate promoters; GFP backbone (Used for RNA binding/tethering)

   Depositor

   Jennifer Doudna

   Article

   Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

   Insert

   FLAG-NLS-Csm1; FLAG-NLS-Csm2; FLAG-NLS-Csm3(RNase mut); FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA; GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. JunD

   Plasmid

   #187904

   Purpose

   Expresses JunD in transfected cells

   Depositor

   Jennifer Mitchell

   Article

   Mitchell et al Endocrinology. 2005 Apr;146(4):2048-54.

   Insert

   JunD (Jund Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCF525-EF1a-Hygro-P2A-AcrIIA4-lenti

   Plasmid

   #115795

   Purpose

   Expresses AcrIIA4 in human cells, contained in a lentiviral vector.

   Depositor

   Jennifer Doudna

   Article

   Watters et al Science. 2018 Oct 12;362(6411):236-239.

   Insert

   AcrIIA4
   

   Use

   Lentiviral

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   EF-1a promoter

   Available since

   Oct. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLBH559_Tet-HisCas14a1Locus

   Plasmid

   #112502

   Purpose

   Expresses 10xHis-Cas14a1 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoter

   Depositor

   Jennifer Doudna

   Article

   Harrington et al Science. 2018 Oct 18. pii: science.aav42

   Insert

   Cas14a1
   

   Use

   CRISPR

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Tet

   Available since

   Oct. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBA620

    Plasmid

    #186240

    Purpose

    LbuCas13a Negative Control (RFP targeting)

    Depositor

    Jennifer Doudna

    Article

    Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

    Insert

    pTet-eLbuCas13a + crRNA negative control
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBA630

    Plasmid

    #186241

    Purpose

    RfxCas13d Negative Control (RFP targeting)

    Depositor

    Jennifer Doudna

    Article

    Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

    Insert

    pTet-RfxCas13d + crRNA negative control
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. 4xNLS-pMJ915v2-sfGFP

    Plasmid

    #88921

    Purpose

    For expression of modified SpyCas9 in e.coli.

    Depositor

    Jennifer Doudna

    Article

    Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Inserts

    Cas9
    T7
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. p2CT-His-MBP-Lba_Cas13a_WT

    Plasmid

    #91867

    Purpose

    Bacterial expression for Cas13a

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

    Insert

    LbaCas13a (LbaC2c2) WT
    

    Use

    Tags

    His6-MBP-TEV site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCF525-EF1a-Hygro-P2A-mTagBFP2-lenti

    Plasmid

    #115797

    Purpose

    Expresses mTagBFP in human cells, contained in a lentiviral vector.

    Depositor

    Jennifer Doudna

    Article

    Watters et al Science. 2018 Oct 12;362(6411):236-239.

    Insert

    mTagBFP
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    EF-1a promoter

    Available since

    Oct. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBA562

    Plasmid

    #186237

    Purpose

    pTet-RfxCas13d crRNA entry vector

    Depositor

    Jennifer Doudna

    Article

    Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLBH545_Tet-Cas14a1_Locus

    Plasmid

    #112501

    Purpose

    Expresses Cas14a1 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoter

    Depositor

    Jennifer Doudna

    Article

    Harrington et al Science. 2018 Oct 18. pii: science.aav42

    Insert

    Cas14a1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Tet

    Available since

    Oct. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPP444

    Plasmid

    #158802

    Purpose

    Plasmid containing Homo sapiens codon optimized CasΦ-3 and a SapI-GG stuffer spacer for editing in human cells.

    Depositor

    Jennifer Doudna

    Article

    Pausch et al Science. 2020 Jul 17;369(6501):333-337.

    Insert

    CasPhi-3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBA615

    Plasmid

    #186239

    Purpose

    eLbuCas13a Negative Control (RFP targeting)

    Depositor

    Jennifer Doudna

    Article

    Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

    Insert

    pTet-LbuCas13a + crRNA negative control
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. LbCas12a-gRNA_BbsI_CAM

    Plasmid

    #183222

    Purpose

    LbCas12a gRNA containing BbsI restriction recognition sites in spacer sequence for Golden Gate Assembly

    Depositor

    Jennifer Doudna

    Article

    Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

    Insert

    LbCas12a guide RNA containing BbsI sites in spacer sequence
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMBP-AaCas12b

    Plasmid

    #113433

    Purpose

    Expresses Expresses 10xHis-MBP fused to AaCas12b

    Depositor

    Jennifer Doudna

    Article

    Chen et al Science. 2018 Apr 27;360(6387):436-439.

    Insert

    AaCas12b
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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