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pEpicVector3mC_Puro-T2A-mCherry-StrepTagII-ProtC-HA

Plasmid

#162109

Purpose

mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

Depositor

Julien Sage

Article

Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

Insert

Puromycin resistant; mCherry linked to StrepTagII-ProtC-HA

Use

Lentiviral

Tags

StrepTagII-ProtC-HA

Mutation

WT

Promoter

EF1A

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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HA-Rab8a-DN (T22N)

Plasmid

#101049

Purpose

Expresses HA-tagged human Rab8-DN (T22N) in mammalian cells

Depositor

Ken-Ichi Takemaru

Article

Burke et al J Cell Biol. 2014 Oct 13;207(1):123-37.

Insert

Rab8a (RAB8A Human)

Tags

HA

Expression

Mammalian

Mutation

T22N

Promoter

CMV

Available Since

Oct. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX FynA-SH2

Plasmid

#46429

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

FynA (FYN Human)

Tags

AviTag, GST, and PreScission

Expression

Bacterial

Mutation

contains amino acids 142-251

Promoter

Tac

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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STARR-seq luciferase INF enhancer vector_ORI_IFIT2

Plasmid

#99321

Purpose

Luciferase validation vector with IFIT2 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity

Depositor

Alexander Stark

Article

Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

Insert

enhancer; chr10: 91060605-91062447

Use

Luciferase; Starr-seq luciferase validation vector

Expression

Mammalian

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-FNLS-PGK-Puro

Plasmid

#110844

Purpose

Lentiviral vector for constitutive expression of FNLS in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3RA-FNLS

Use

Lentiviral

Tags

3X FLAG

Mutation

D10A and NLS sequence at the N-terminus

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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hIL23p19 pEF6-V5 (MJS_004)

Plasmid

#72492

Purpose

Expression of human IL23p19 in mammalian cells (V5 tag)

Depositor

Matthew Sweet

Article

Ramnath et al Immunol Cell Biol. 2015 Oct;93(9):771-9.

Insert

IL23p19 (IL23A Human)

Tags

His/V5

Expression

Mammalian

Available Since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pEpicVector3mC_Puro-T2A-mCherry-VSVg-StrepTagII-FLAG

Plasmid

#162101

Purpose

mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

Depositor

Julien Sage

Article

Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

Insert

Puromycin resistant; mCherry linked to VSVg-StrepTagII-FLAG

Use

Lentiviral

Tags

VSVg-StrepTagII-FLAG

Mutation

WT

Promoter

EF1A

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pEpicVector3mC_Puro-T2A-mCherry-VSVg-ProtC-AU1

Plasmid

#162105

Purpose

mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

Depositor

Julien Sage

Article

Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

Insert

Puromycin resistant; mCherry linked to VSVg-ProtC-AU1

Use

Lentiviral

Tags

VSVg-ProtC-AU1

Mutation

WT

Promoter

EF1A

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pEpicVector3mC_Puro-T2A-mCherry-StrepTagII-FLAG-AU1

Plasmid

#162114

Purpose

mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

Depositor

Julien Sage

Article

Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

Insert

Puromycin resistant; mCherry linked to StrepTagII-FLAG-AU1

Use

Lentiviral

Tags

StrepTagII-FLAG-AU1

Mutation

WT

Promoter

EF1A

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pEpicVector3mC_Puro-T2A-mCherry-ProtC-HA-FLAG

Plasmid

#162115

Purpose

mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

Depositor

Julien Sage

Article

Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

Insert

Puromycin resistant; mCherry linked to ProtC-HA-FLAG

Use

Lentiviral

Tags

ProtC-HA-FLAG

Mutation

WT

Promoter

EF1A

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pEpicVector3G_Puro-T2A-EGFP-VSVg-HA-FLAG

Plasmid

#162086

Purpose

EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

Depositor

Julien Sage

Article

Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

Insert

Puromycin resistant; EGFP linked to VSVg-HA-FLAG

Use

Lentiviral

Tags

VSVg-HA-FLAG

Mutation

WT

Promoter

EF1A

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pEpicVector3G_Puro-T2A-EGFP-StrepTagII-HA-AU1

Plasmid

#162093

Purpose

EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

Depositor

Julien Sage

Article

Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

Insert

Puromycin resistant; EGFP linked to StrepTagII-HA-AU1

Use

Lentiviral

Tags

StrepTagII-HA-AU1

Mutation

WT

Promoter

EF1A

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pEpicVector3G_Puro-T2A-EGFP-ProtC-HA-AU1

Plasmid

#162096

Purpose

EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

Depositor

Julien Sage

Article

Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

Insert

Puromycin resistant; EGFP linked to ProtC-HA-AU1

Use

Lentiviral

Tags

ProtC-HA-AU1

Mutation

WT

Promoter

EF1A

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pEpicVector3G_Puro-T2A-EGFP-VSVg-StrepTagII-ProtC

Plasmid

#162079

Purpose

EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

Depositor

Julien Sage

Article

Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

Insert

Puromycin resistant; EGFP linked to VSVg-StrepTagII-ProtC

Use

Lentiviral

Tags

VSVg-StrepTagII-ProtC

Mutation

WT

Promoter

EF1A

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pEpicVector3G_Puro-T2A-EGFP-VSVg-ProtC-HA

Plasmid

#162083

Purpose

EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

Depositor

Julien Sage

Article

Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

Insert

Puromycin resistant; EGFP linked to VSVg-ProtC-HA

Use

Lentiviral

Tags

VSVg-ProtC-HA

Mutation

WT

Promoter

EF1A

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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STK11 gRNA (BRDN0001147319)

Plasmid

#75914

Purpose

3rd generation lentiviral gRNA plasmid targeting human STK11

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

STK11 (STK11 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX Stat3-SH2

Plasmid

#46513

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Stat3 (STAT3 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 584-700

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pMDC43-FIT2

Plasmid

#96993

Purpose

Express GFP-tagged mouse FIT2 gene in plants.

Depositor

Kent Chapman

Article

Cai et al Plant Biotechnol J. 2016 Dec 17. doi: 10

Insert

MmFIT2 (Fitm2 Mouse)

Tags

GFP

Expression

Plant

Mutation

E2D (please see depositor comments below)

Promoter

35S

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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SARS-COV2-NSP1-HA-BASU

Plasmid

#154071

Purpose

Express SARS-CoV-2 NSP1 gene along with HA and BASU

Depositor

Paul Khavari

Article

Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

Insert

Nsp1 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pDGB3 alpha2 P35S:MS2:VPR:Tnos (GB1830)

Plasmid

#160624

Purpose

TU for the constitutive expression of the MS2 coat protein fused on Ct to the activation domain VPR (VP64+p65+Rta)

Depositor

Diego Orzaez

Article

Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

Insert

P35S:MS2:VPR:Tnos

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

P35S

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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