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Anti-Beta4-spectrin [N393/2R]

Plasmid

#177541

Purpose

Mammalian Expression Plasmid of anti-Beta4-spectrin (Human). Derived from hybridoma N393/2.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Beta4-spectrin (Homo sapiens) recombinant mouse monoclonal antibody (SPTBN4 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pUltraHot-mCherry-BLM-WT

Plasmid

#206966

Purpose

expressing the protein BLM fused to mCherry in human cells

Depositor

Eran Hornstein

Article

Danino et al Nucleic Acids Res. 2023 Jul 28:gkad613.

Insert

Bloom Syndrome RecQ Like Helicase (BLM Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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IP3 receptor, type 1 scFv [L24/1]

Plasmid

#206755

Purpose

Mammalian Expression of IP3 receptor, type 1 scFV. Derived from hybridoma L24/1 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

IP3 receptor, type 1 (Rattus norvegicus) recombinant scFV (Itpr1 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pYX-N-Puro-mNeon-WNK3

Plasmid

#222674

Purpose

endogenously tag WNK3 with N-terminal mNeon

Depositor

Jason Moffat

Article

Xiao et al Cell Rep. 2024 Jul 23;43(7):114417. doi:

Insert

WNK3 (WNK3 Human)

Tags

mNeon

Expression

Bacterial

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-Nav1.4 Na+ channel [N255/38]

Plasmid

#190306

Purpose

Mammalian Expression Plasmid of anti-Nav1.4 Na+ channel (Rat). Derived from hybridoma N255/38.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Nav1.4 Na+ channel (Rattus norvegicus) recombinant Mouse monoclonal antibody (Scn4a Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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8798 GFP-KrasG12C_2B_hygro

Plasmid

#64376

Purpose

This a retroviral expression plasmid expressing GFP tagged G12C mutant Kras 2B oncogene along with hygro resistance gene

Depositor

Georgios Stathopoulos

Article

Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

Insert

mouse Kras2B (Kras Mouse)

Use

Retroviral

Tags

GFP

Mutation

changed glycine 12 to cysteine

Available Since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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pDEST-Myc-FBW7 Delta F-Box

Plasmid

#197452

Purpose

The plasmid expresses F-box deleted version of Myc-tagged FBW7 human isoform 1. Used for mammalian overexpression of FBW7alpha delta-F-Box and detection by western blotting.

Depositor

Omar Khan

Article

Khan et al Nat Commun. 2021 Apr 6;12(1):2043. doi:

Insert

FBW7 (FBXW7 Human)

Tags

Myc

Expression

Mammalian

Mutation

FBW7 Fbox deleted.

Promoter

CMV

Available Since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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pVD1 Multiplexing Dicot Edit E1_NTERM (GB2245)

Plasmid

#160567

Purpose

tRNA and scaffold for the assembly of GBoligomers for position [D1_n] of a monocistronic tRNA-gRNA

Depositor

Diego Orzaez

Article

Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d

Insert

Multiplexing Dicot Edit E1_NTERM (Multiplexing Dicot Edit)

Use

CRISPR and Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-TrpC6 [N490/24R]

Plasmid

#211590

Purpose

Mammalian Expression Plasmid of anti-TrpC6 (Human) IgG2a R-mAb. Derived from hybridoma N490/24.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-TrpC6 (Homo sapiens) recombinant mouse monoclonal antibody. (TRPC6 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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8047_GFP-Kras2B_ires_puro

Plasmid

#64371

Purpose

This a retroviral expression plasmid expressing GFP tagged wild type Kras 2B oncogene along with puro resistance gene

Depositor

Georgios Stathopoulos

Article

Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

Insert

mouse Kras2B (Kras Mouse)

Use

Retroviral

Tags

GFP

Mutation

none

Available Since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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Anti-Lgi1 [N283/7R]

Plasmid

#177527

Purpose

Mammalian Expression Plasmid of anti-Lgi1 (Mouse). Derived from hybridoma N283/7R.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Lgi1 (Mus musculus) recombinant mouse monoclonal antibody (Lgi1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-GFAP [N206A/8R-2b]

Plasmid

#199410

Purpose

Mammalian expression plasmid of Anti-GFAP [N206A/8R-2b] (Human). Derived from hybridoma N206A/8.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-GFAP (Human) recombinant mouse monoclonal antibody (GFAP Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pαH-S-GSAS-B.1.1.7

Plasmid

#171747

Purpose

Mammalian expression of SARS-CoV-2 Spike protein S-GSAS-B.1.1.7 variant (UK)

Depositor

Priyamvada Acharya

Article

Gobeil et al Science. 2021 Jun 24. pii: science.abi62

Insert

Spike (S-GSAS-B.1.1.7 variant) (S Severe acute respiratory syndrome coronavirus 2)

Tags

HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

Expression

Mammalian

Mutation

Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

Available Since

July 16, 2021

Availability

Academic Institutions and Nonprofits only

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GluN2B/NR2B glutamate receptor scFv [N59/20]

Plasmid

#182092

Purpose

Mammalian Expression of GluN2B/NR2B glutamate receptor scFv. Derived from hybridoma N59/20.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting GluN2B/NR2B glutamate receptor (Rattus norvegicus) (Grin2b Mouse)

Tags

Sortase, His

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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JDW 743 (pDONR221-EYFP-hKRAS4B-WT)

Plasmid

#156402

Purpose

Gateway middle entry clone encoding EYFP-fused to human KRAS4B, includes a stop codon

Depositor

Joshua Wythe

Article

Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

Insert

human wild type KRAS4B-WT (KRAS Human)

Use

Gateway cloning

Tags

EYFP

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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C-Fos scFv [N486/32]

Plasmid

#182090

Purpose

Mammalian Expression of C-Fos scFv. Derived from hybridoma N486/32.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting C-Fos (Homo sapiens) (FOS Mouse)

Tags

HA, His

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-VGlut1 [N28/9R-rat IgG2a]

Plasmid

#222184

Purpose

Mammalian Expression Plasmid of anti-VGlut1 neurotransmitter transporter (Rat). Derived from hybridoma N28/9.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-VGlut1 neurotransmitter transporter (Rattus norvegicus) recombinant mouse monoclonal antibody. (Slc17a6 Chimera: M. musculus (mouse)/R. norvegicus (rat))

Expression

Mammalian

Promoter

Dual CMV

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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SUR1 scFv [N289/16]

Plasmid

#206735

Purpose

Mammalian Expression of SUR1 scFV. Derived from hybridoma N289/16 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

SUR1 (Rattus norvegicus) recombinant scFV (Abcc8 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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Ankyrin-G scFv [N106/36]

Plasmid

#199422

Purpose

Mammalian expression of Ankyrin-G scFv. Derived from hybridoma N106/36.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targetingAnkyrin-G (Human) (ANK3 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pCAGGS/XBB.1.5-RBD

Plasmid

#213038

Purpose

Mammalian cell expression of SARS-CoV-2 Spike RBD protein of OMICRON.XBB.1.5 variant

Depositor

Priyamvada Acharya

Article

Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

Insert

pCAGGS/XBB.1.5-RBD (S )

Tags

6 His Tag

Expression

Mammalian

Mutation

G339H,R346T,L368I,S371F,S373P,S375F,T376A,D405N,R…

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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