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TRIP8b (constant) ion channel scFv [N212/17]

Plasmid

#206725

Purpose

Mammalian Expression of TRIP8b (constant) ion channel scFV. Derived from hybridoma N212/17 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

TRIP8b (constant) ion channel (Rattus norvegicus) recombinant scFV (Pex5l Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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Shank1 and Shank3 scFv [N367/51]

Plasmid

#206728

Purpose

Mammalian Expression of Shank1 and Shank3 scaffold protein scFV. Derived from hybridoma N367/51 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Shank1 and Shank3 scaffold protein (Rattus norvegicus) recombinant scFV (Shank3 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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Kv1.5 K+ channel scFv [K7/45]

Plasmid

#206730

Purpose

Mammalian Expression of Kv1.5 K+ channel scFV. Derived from hybridoma K7/45 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Kv1.5 K+ channel (Rattus norvegicus) recombinant scFV (Kcna5 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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GABA(B)R1 scFv [N93A/49]

Plasmid

#206731

Purpose

Mammalian Expression of GABA(B)R1 scFV. Derived from hybridoma N93A/49 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

GABA(B)R1 (Rattus norvegicus) recombinant scFV (Gabbr1 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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nNOS/NOS1 scFv [L121/136]

Plasmid

#206758

Purpose

Mammalian Expression of nNOS/NOS1 scFV. Derived from hybridoma L121/136 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

nNOS/NOS1 (Homo sapiens) recombinant scFV (NOS1 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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Anti-VAChT [N6/38R-1]

Plasmid

#206716

Purpose

Mammalian Expression Plasmid of anti-VAChT (Human). Derived from hybridoma N6/38-1.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-VAChT (Homo sapiens) recombinant Mouse monoclonal antibody (SLC18A3 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Oct. 20, 2023

Availability

Academic Institutions and Nonprofits only

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Anti-Pannexin-2 [N121A/1R]

Plasmid

#206527

Purpose

Mammalian Expression Plasmid of anti-Pannexin-2 (Rat). Derived from hybridoma N121A/1.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Pannexin-2 (Rattus norvegicus) recombinant Mouse monoclonal antibody (Panx2 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST-Myc-FBW7 Delta D-Domain

Plasmid

#197453

Purpose

The plasmid expresses D-domain deleted version of Myc-tagged FBW7 human isoform 1. Used for mammalian overexpression and detection by western blotting.

Depositor

Omar Khan

Article

Khan et al Nat Commun. 2021 Apr 6;12(1):2043. doi:

Insert

FBW7 (FBXW7 Human)

Tags

Myc

Expression

Mammalian

Mutation

FBW& dimerization domain deleted.

Promoter

CMV

Available Since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST-Myc-FBW7K404/412/R

Plasmid

#200716

Purpose

The plasmid expresses Myc-tagged FBW7 human isoform 1 with Lys404 & 412 to Arg mutation. Used for mammalian overexpression and detection by western blotting.

Depositor

Omar Khan

Article

Khan et al Nat Commun. 2021 Apr 6;12(1):2043. doi:

Insert

FBW7 (FBXW7 Human)

Tags

Myc

Expression

Mammalian

Mutation

FBW7 K404 and K412 mutated to Alanines.

Promoter

CMV

Available Since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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2X_pX458_pSpCas9(BB)-2A-GFP_eSOX17.2_KO

Plasmid

#195495

Purpose

Cas9-2A-GFP expression vector bearing two sgRNAs targeting the core endoderm distal enhancer of human SOX17

Depositor

Alexander Meissner

Article

Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

Insert

sgRNA (SOX17 Human)

Use

CRISPR

Tags

3XFLAG and GFP

Expression

Mammalian

Promoter

U6

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV10-3xFLAG-prs-hMIGA2(170-593)-25GS-6xhis

Plasmid

#192835

Purpose

Express codon-optimized human MIGA2 fragment 170-593 with a GS-linker and 6xhis tag at C-terminus (lipid transfer construct) in Expi293 cells

Depositor

Karin Reinisch

Article

Hong et al J Cell Biol. 2022 Dec 5;221(12):e2022070

Insert

Codon-optimized human Mitoguardin 2 (MIGA2 Human)

Tags

3xFLAG-PreScission cleavage site and 6xhis

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCV Cdk2ap1ΔN (MT2B2)-HA

Plasmid

#178031

Purpose

Retroviral vector for the purpose of overexpression in mammalian cell culture

Depositor

Lin He

Article

Modzelewski et al Cell. 2021 Oct 7. pii: S0092-8674(21)011

Insert

Cdk2ap1 (Cdk2ap1 Mouse)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

First N-Terminal 27aa are deleted

Promoter

GAG

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-Pan-Kvbeta K+ channel [K40/17R]

Plasmid

#188191

Purpose

Mammalian Expression Plasmid of anti-Pan-Kvbeta K+ channel. Derived from hybridoma K40/17

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Pan-Kvbeta K+ channel recombinant mouse monoclonal antibody

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-Synaptotagmin-9 [N276A/15R]

Plasmid

#188223

Purpose

Mammalian Expression Plasmid of anti-Synaptotagmin-9 (Mouse). Derived from hybridoma N276A/15

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Synaptotagmin-9 (Mus musculus) recombinant mouse monoclonal antibody (Syt9 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Pan-Gamma-protocadherin-A scFv [N144/32]

Plasmid

#190532

Purpose

Mammalian Expression of Pan-Gamma-protocadherin-A scFV. Derived from hybridoma N144/32.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Pan-Gamma-protocadherin-A (Mus musculus) recombinant scFV (Pcdhga3 Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Pan-Gamma-protocadherin-Constant scFv [N159/5]

Plasmid

#190533

Purpose

Mammalian Expression of Pan-Gamma-protocadherin-Constant scFV. Derived from hybridoma N159/5.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Pan-Gamma-protocadherin-Constant (Mus musculus) recombinant scFV (Pcdhga1 Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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TRIP8b (exon 1b) scFv [N212A/34]

Plasmid

#190538

Purpose

Mammalian Expression of TRIP8b (exon 1b) scFV. Derived from hybridoma N212A/34.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

TRIP8b (exon 1b) (Mus musculus) recombinant scFV (Pex5l Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-Synaptotagmin-6 [N270/47R]

Plasmid

#188216

Purpose

Mammalian Expression Plasmid of anti-Synaptotagmin-6 (Mouse). Derived from hybridoma N270/47

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Synaptotagmin-6 (Mus musculus) recombinant mouse monoclonal antibody (Syt6 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Pan-Shank scFv [N23B/49]

Plasmid

#190542

Purpose

Mammalian Expression of Pan-Shank scFV. Derived from hybridoma N23B/49.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Pan-Shank (Rattus norvegicus) recombinant scFV (Shank2 Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-Gamma-protocadherin-B2 [N148/30]

Plasmid

#190299

Purpose

Mammalian Expression Plasmid of anti-Gamma-protocadherin-B2 (Mouse). Derived from hybridoma N148/30.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Gamma-protocadherin-B2 (Mus musculus) recombinant Mouse monoclonal antibody (Pcdhgb2 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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