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pCMV-HA-mIFITM3-N2A

Plasmid

#58410

Purpose

Expresses murine IFITM3-N2A with an N-terminal HA tag in mammalian cells (mutates possible glycosylation site)

Depositor

Howard Hang, Jacob Yount

Article

Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

Insert

IFITM3 (Ifitm3 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Asparagine 2 to Alanine

Promoter

CMV IE

Available since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-HA-mIFITM3-K24,83,104A

Plasmid

#58407

Purpose

Expresses murine IFITM3-K24,83,104A with an N-terminal HA tag in mammalian cells

Depositor

Howard Hang, Jacob Yount

Article

Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

Insert

IFITM3 (Ifitm3 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Lysines 24, 83, and 104 to Alanine

Promoter

CMV IE

Available since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-HA-mIFITM3-S38N

Plasmid

#58411

Purpose

Expresses murine IFITM3-S38N with an N-terminal HA tag in mammalian cells (adds a possible glycosylation site)

Depositor

Howard Hang, Jacob Yount

Article

Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

Insert

IFITM3 (Ifitm3 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Serine 38 to Asparagine

Promoter

CMV IE

Available since

Sept. 5, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-HA-mIFITM3-K24,83,88A

Plasmid

#58404

Purpose

Expresses murine IFITM3-K24,83,88A with an N-terminal HA tag in mammalian cells

Depositor

Howard Hang, Jacob Yount

Article

Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

Insert

IFITM3 (Ifitm3 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Lysines 24, 83, and 88 to Alanine

Promoter

CMV IE

Available since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-HA-mIFITM3-K88A

Plasmid

#58402

Purpose

Expresses murine IFITM3-K88A with an N-terminal HA tag in mammalian cells

Depositor

Howard Hang, Jacob Yount

Article

Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

Insert

IFITM3 (Ifitm3 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Lysine 88 to Alanine

Promoter

CMV IE

Available since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-HA-mIFITM3-K104A

Plasmid

#58403

Purpose

Expresses murine IFITM3-K104A with an N-terminal HA tag in mammalian cells

Depositor

Howard Hang, Jacob Yount

Article

Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

Insert

IFITM3 (Ifitm3 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Lysine 104 to Alanine

Promoter

CMV IE

Available since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only
DIRAS1

Plasmid

#55669

Purpose

expression of mouse DIRAS1

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

DIRAS1 (Diras1 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

lac

Available since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pHL 1035

Plasmid

#52999

Purpose

pLtetO-1:RhyB, pCon:fhlA::mCherry, pLlacO-1:sodB::gfp

Depositor

Han Lim

Article

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Inserts

RhyB sRNA
fhlA
sodB

Use

Synthetic Biology

Tags

gfp and mCherry

Expression

Bacterial

Mutation

contains −309 to +96 bp relative to start codon a…

Promoter

pCon, pLlacO-1, and pLtetO-1

Available since

May 16, 2014

Availability

Academic Institutions and Nonprofits only
pHL 1033

Plasmid

#52998

Purpose

pLtetO-1:RhyB, pCon:rpoS::mCherry, pLlacO-1:sodB::gfp

Depositor

Han Lim

Article

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Inserts

RhyB sRNA
rpoS
sodB

Use

Synthetic Biology

Tags

gfp and mCherry

Expression

Bacterial

Mutation

contains −150 and +30 bp relative to start codon …

Promoter

pCon, pLlacO-1, and pLtetO-1

Available since

May 16, 2014

Availability

Academic Institutions and Nonprofits only
pHL 1073

Plasmid

#53003

Purpose

pCon:ompC::mCherry, pLlacO-1:sodB::gfp

Depositor

Han Lim

Article

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Inserts

mCherry
GFP

Use

Synthetic Biology

Tags

ompC (target mRNA) and sodB (target mRNA)

Expression

Bacterial

Mutation

contains −56 to +141 bp relative to start codon a…

Promoter

pCon and pLlacO-1

Available since

May 16, 2014

Availability

Academic Institutions and Nonprofits only
pHL 1303

Plasmid

#53008

Purpose

pLtetO-1:sodB::mCherry, pLlacO-1:ompC::gfp

Depositor

Han Lim

Article

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Inserts

sodB
ompC

Use

Synthetic Biology

Tags

GFP and mCherry

Expression

Bacterial

Mutation

contains -56 to +141 relative to start codon and …

Promoter

pLlacO-1 and pLtetO-1

Available since

May 14, 2014

Availability

Academic Institutions and Nonprofits only
pVRc04_1609

Plasmid

#49732

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

AS04_1609

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pLux

Available since

Jan. 14, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pVRc33_423

Plasmid

#49749

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

AS33_423

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pLux

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pVRc02_915

Plasmid

#49729

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

AS02_915

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pLux

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pVRa39_2973

Plasmid

#49700

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF39_2973

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pVRa17_1458

Plasmid

#49671

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF17_1458

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pVRc37_2513

Plasmid

#49751

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

AS37_2513

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pLux

Available since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pVRc33_375

Plasmid

#49748

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

AS33_375

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pLux

Available since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pVRc27_4265

Plasmid

#49745

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

AS27_4265

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pLux

Available since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pVRc27_1331

Plasmid

#49744

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

AS27_1331

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pLux

Available since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge