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pAT9748-dCBE

Plasmid

#163008

Purpose

plasmid expressing CBE with dCas9

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

dCBE

Use

CRISPR

Tags

3xFLAG and SV40 NLS

Expression

Mammalian

Promoter

EF1-a

Available Since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-U6sgbbBsmbI-puro-2A-Fluc

Plasmid

#100277

Purpose

Lentiviral vector for CRISPR mediated gene editing in cells while expressing Firefly Luciferase for in vivo imaging

Depositor

Sidi Chen

Article

Chow et al Nat Neurosci. 2017 Aug 14. doi: 10.1038/

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

Oct. 26, 2018

Availability

Academic Institutions and Nonprofits only

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MiniCoopR U6:gRNA cdkn2a, mitfa:Cas9

Plasmid

#118842

Purpose

Targets cdkn2a and expresses zebrafish mitfa specifically in zebrafish melanocytes

Depositor

Leonard Zon

Article

Ablain et al Science. 2018 Nov 1. pii: science.aau650

Insert

cdkn2a gRNA (LOC100329528 Zebrafish)

Use

CRISPR; Tol2

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pBAD-HisD-KRaION2

Plasmid

#177800

Purpose

Bacterial expression of green fluorescent potassium indicator KRaION2

Depositor

Edward Boyden

Article

Torres Cabán et al ACS Sens. 2022 May 27;7(5):1336-1346. do

Insert

KRaION2

Expression

Bacterial

Promoter

araB

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAC-aZEA

Plasmid

#53286

Purpose

Contains crtE, and crtB genes of Erwinia herbicola (Pantoea agglomerans) Eho10, crtI gene of Rhodobacter capsulatus, and lcyE cDNA of Arabidopsis thaliana and produces alpha-zeacarotene in E. coli

Depositor

Francis X Cunningham Jr

Article

Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

Insert

lcyE (LUT2 Mustard Weed)

Use

Low copy number bacterial cloning vector

Promoter

lac

Available Since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pVAX_CBI_AcrIIA4

Plasmid

#136662

Purpose

Expresses human codon-optimized AcrIIA4 in mammalian cells. ORF flanked by NotI and XhoI restriction sites

Depositor

Yannick Doyon

Article

Hynes et al Nat Commun. 2018 Jul 25;9(1):2919. doi:

Insert

AcrIIA4

Use

Synthetic Biology

Tags

No tag, No NLS

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pUB-spHRed

Plasmid

#84734

Purpose

Expresses pHRed in plant cells-biosensor for pHRed targeted to chloroplasts stoma

Depositor

Philip Mullineaux

Article

Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

Insert

pHRed

Tags

RbcS targeting sequence

Expression

Plant

Promoter

ubiqutin- 10 gene promoter (pUBQ10) of Arabidopsis

Available Since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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pCfB2794

Plasmid

#63651

Purpose

pCfB2794 is a vector for multiple integrations at sites sharing homology with Ty1Cons2. The selective marker is Kl.URA3. Additionally it contains a PTEF1-GFP as reporter.

Depositor

Irina Borodina, Jerome Maury

Article

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Insert

GFP

Use

Cre/Lox

Expression

Yeast

Promoter

TEF1 promoter

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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His-TEV-Aurein1.2_(+36GFP)-LPETG-His

Plasmid

#71755

Purpose

His-TEV-Aurein1.2+36GFP-LPETG-His expression in E.Coli

Depositor

David Liu

Article

Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

Insert

Aurein1.2_(pos36_GFP)-LPETG-His

Tags

6XHis, Sortase (LPETG), and TEV Protease

Expression

Bacterial

Available Since

Dec. 18, 2015

Availability

Academic Institutions and Nonprofits only

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PERK 4: PERKKD-pGEX4T-1

Plasmid

#21817

Depositor

David Ron

Article

Harding et al Nature. 1999 Jan 21. 397(6716):271-4.

Insert

PERK (537-1114) (Eif2ak3 Mouse)

Tags

GST and Myc (9E10)

Expression

Bacterial

Mutation

PERK Kinase Domain. Amino acids 537-1114.

Available Since

March 29, 2010

Availability

Academic Institutions and Nonprofits only

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pIA1362

Plasmid

#166860

Purpose

Expression of codon optimized SARS-COV-2 Nsp7 in E. coli

Depositor

Irina Artsimovitch

Article

Wang et al mBio. 2021 Jun 29;12(3):e0142321. doi: 1

Insert

SARS-COV-2 Nsp7 (ORF1ab Synthetic)

Tags

His-GB1

Expression

Bacterial

Promoter

T7

Available Since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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pYFP-NEAT1m_v1-RT

Plasmid

#97089

Purpose

Encodes a HR repair template for knock-in a YFP expression cassette downstream of the poly(A) signal of human NEAT1_1 isoform. Best used with px330-NEAT1m_v1 vector.

Depositor

Archa Fox

Article

Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

Insert

NEAT1m_v1-RT (NEAT1 Human)

Use

CRISPR

Tags

EYFP

Promoter

CMV

Available Since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

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pDGE476

Plasmid

#153267

Purpose

M2E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp-LZ-mNeongreen

Plasmid

#182392

Purpose

Bacterial expression of Vesicle Nucleating peptide-Leucine Zipper-mNeongreen fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp-LZ-mNeongreen

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pET24a_SMOXdel

Plasmid

#186785

Purpose

C-terminal His-Tag and a TEV cleaveage site, deletion of 8 residues (503-512) to transform into E. coli BL21 (DE3) cells

Depositor

Sandra Gabelli

Article

Dunston et al Acta Naturae. 2020 Jul-Sep;12(3):140-144

Insert

Spermine oxidase (SMOX Human)

Tags

His tag

Expression

Bacterial

Mutation

deleted amino acids 503-512

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pYFP-NEAT1_IS_RT

Plasmid

#97091

Purpose

Encodes a HR repair template that mutates the poly(A) signal as well as a few splicing factor binding sites of human NEAT1_1 isoform. Best used with px335-NEAT1_IS_v1 and v2 vectors.

Depositor

Archa Fox

Article

Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

Insert

NEAT1_IS_RT (NEAT1 Human)

Use

CRISPR

Promoter

CMV

Available Since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2-KLF7-1

Plasmid

#169797

Purpose

Expresses Cas9 and guide RNA for the site neighbouring KLF7 gene.

Depositor

Natsuko Chiba

Article

Yoshino et al Sci Rep. 2019 Feb 7;9(1):1644. doi: 10.1

Insert

guide RNA for KLF7 neighbouring region

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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pATP403red-hENT1

Plasmid

#166027

Purpose

Expresses human equilibrative nucleoside transporter 1 (hENT1)

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

Insert

human equilibrative nucleoside transporter 1 (SLC29A1 Human)

Use

Synthetic Biology

Expression

Yeast

Promoter

pTDH3

Available Since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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pCPP5951

Plasmid

#128719

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

Depositor

Alan Collmer

Article

Kvitko et al PLoS Pathog. 2009 Apr;5(4):e1000388. doi

Insert

hopR1

Expression

Bacterial

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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penaav-tnt-3xha-p2a-gfp

Plasmid

#175090

Purpose

For making AAV9

Depositor

William Pu

Article

Akerberg et al Nat Commun. 2019 Oct 28;10(1):4907. doi:

Type

Empty backbone

Use

AAV

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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