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pVF26

Plasmid

#229458

Purpose

Expresses IraD with Q84A mutation with His-tag

Depositor

Alexandra Deaconescu

Publication

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

IraD (Q84A)

Tags

6xHis

Expression

Bacterial

Mutation

Q84A

Promoter

T7

Available since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pVF25

Plasmid

#229457

Purpose

Expresses IraD with R67A mutation with His-tag

Depositor

Alexandra Deaconescu

Publication

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

IraD (R67A)

Tags

6xHis

Expression

Bacterial

Mutation

R67A

Promoter

T7

Available since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pNK7185

Plasmid

#219765

Purpose

Vector for expression PzPKS2 in yeast (P.pastoris)

Depositor

Karen Sarkisyan

Publication

Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

Insert

PzPKS2

Expression

Yeast

Promoter

pGAP

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pNK7190

Plasmid

#219763

Purpose

MoClo-compatible Level M for expression nnLuz, nnH3H, nnCPH in plants (N.benthamiana, BY2 cells)

Depositor

Karen Sarkisyan

Publication

Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

Insert

nnLuz, nnH3H, nnCPH

Expression

Plant

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pBSII-FRT-MCS

Plasmid

#205988

Purpose

Vector containing FRT and FRT-F3 sites

Depositor

Sharon Torigoe

Publication

Waite et al PLoS One. 2024 Sep 30;19(9):e0311120. do

Type

Empty backbone

Use

Pcr vector

Available since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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HisMS2_VLP_SC2

Plasmid

#175957

Purpose

Generation of MS2 Virus-Like Particles packaged with the sequence for the SARS-CoV-2 N gene [CoV USA/WA-CDC-WA1/2020]

Depositor

Paul Freemont

Publication

Unruh et al medRxiv 2024.04.17.24305965

Insert

N gene

Expression

Bacterial

Promoter

T7

Available since

Sept. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pSM045

Plasmid

#217399

Purpose

KmR, pBTRcK broad host range vector with BTE1(C12-specific fatty acid thioesterase codon-optimised for C. necator) under Ptrc

Depositor

Brian Pfleger

Publication

Mishra et al J Ind Microbiol Biotechnol. 2024 Feb 16:

Insert

BTE1

Expression

Bacterial

Promoter

PTrc

Available since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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PSMD3_BirA_Nterm

Plasmid

#221526

Purpose

Flag-tagged biotinylation enzyme for co-expression of Psmd3

Depositor

Alessandro Ori

Insert

PSMD3 (PSMD3 Human)

Tags

BirA

Expression

Mammalian

Available since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pNK7181

Plasmid

#219766

Purpose

Vector for expression HmS in yeast (P.pastoris)

Depositor

Karen Sarkisyan

Publication

Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

Insert

HmS

Expression

Yeast

Promoter

pGAP

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pNK7182

Plasmid

#219767

Purpose

Vector for expression PpASCL in yeast (P.pastoris)

Depositor

Karen Sarkisyan

Publication

Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

Insert

PpASCL

Expression

Yeast

Promoter

pGAP

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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AA195

Plasmid

#215984

Purpose

Fragmid fragment: (N' terminus) ALFA tag for recruiting via NbALFA

Depositor

John Doench

Publication

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

Start; BPNLS_v1.1; ALFA_v1.3; ALFA_v1.2; ALFA_v1.1; ALFA_v1.4; ALFA_v1.5

Use

Fragment

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCDB179-RdhF3

Plasmid

#217823

Purpose

Expresses HisSUMO-RdhF3 in E. coli

Depositor

Pamela Silver

Publication

Ng et al ACS Chem Biol. 2024 Feb 16;19(2):380-391

Insert

RdhF3

Tags

His10xSUMO

Expression

Bacterial

Promoter

T7

Available since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-HIS5

Plasmid

#207014

Purpose

Plasmid backbone for yeast expression, use auxotrophic marker HIS5 (Schizosaccharomyces Pombe)

Depositor

Zhiping Xie

Publication

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Type

Empty backbone

Expression

Yeast

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-URA3

Plasmid

#207012

Purpose

Plasmid backbone for yeast expression, use auxotrophic marker URA3 (Kluyveromyces lactis)

Depositor

Zhiping Xie

Publication

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Type

Empty backbone

Expression

Yeast

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pML646

Plasmid

#206999

Purpose

Contains mAID-tag, mouse Ptbp1-specific homology arms and blasticidin S resistance gene.

Depositor

Eugene Makeyev

Publication

Kainov et al STAR Protoc. 2023 Oct 18;4(4):102644. do

Insert

BSD resistance

Use

Unspecified

Available since

Oct. 20, 2023

Availability

Academic Institutions and Nonprofits only

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BASIC_SEVA_37_pKD46.10

Plasmid

#178927

Purpose

BASIC DNA assembly vector

Depositor

Paul Freemont

Publication

Haines et al Synth Biol (Oxf). 2022 Oct 11;7(1):ysac0

Insert

mScarlet counter-selection cassette

Use

Synthetic Biology

Available since

March 1, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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BASIC_SEVA_26.10

Plasmid

#178919

Purpose

BASIC DNA assembly vector

Depositor

Paul Freemont

Publication

Haines et al Synth Biol (Oxf). 2022 Oct 11;7(1):ysac0

Insert

mScarlet counter-selection cassette

Use

Synthetic Biology

Available since

March 1, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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BASIC_SEVA_15a.10

Plasmid

#178913

Purpose

BASIC DNA assembly vector

Depositor

Paul Freemont

Publication

Haines et al Synth Biol (Oxf). 2022 Oct 11;7(1):ysac0

Insert

mScarlet counter-selection cassette

Use

Synthetic Biology

Available since

Feb. 2, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pUC-TalO8 (Blank-CoTrIP)

Plasmid

#178303

Purpose

Blank CoTrIP plasmid

Depositor

Brian Zid

Publication

Chen et al eLife. 2022 Sep 15;11. pii: 76965. doi:

Type

Empty backbone

Tags

NA

Expression

Yeast

Promoter

NA

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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L0-AA008

Plasmid

#185754

Purpose

Level 0 loop assembly vector containing the Anthoceros agrestis EF1a promoter (pAaEF1a).

Depositor

Peter Szoevenyi

Publication

Frangedakis et al New Phytol. 2021 Jun 2. doi: 10.1111/nph

Insert

pAaEF1a (promoter)

Use

Synthetic Biology

Expression

Plant

Available since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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