We narrowed to 65,548 results for: nin

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SpRYc

Plasmid

#175575

Purpose

Expresses the codon-optimized, engineered SpRYc Cas9 nuclease in mammalian cells

Depositor

Joseph Jacobson

Article

Koseki et al Res Sq. 2023 Mar 7:rs.3.rs-2625838. doi:

Insert

SpRYc

Tags

EGFP

Expression

Mammalian

Mutation

PID of SpRY grafted onto the N-terminal domain of…

Promoter

CMV

Available Since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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PZac2.1 hSyn1-BioID2-BioID2-HA

Plasmid

#176855

Purpose

Expresses the promiscuous small biotin ligase, BioID2, in neurons

Depositor

Baljit Khakh

Article

Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

Insert

BioID2-BioID2-HA

Use

AAV

Tags

HA

Expression

Bacterial

Promoter

hSyn1

Available Since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-MYOD1-WT

Plasmid

#194570

Purpose

Mammalian Expression of mEGFP-MYOD1 WT Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

MYOD1 (MYOD1 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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TARDBP

Plasmid

#156073

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

TARDBP (TARDBP Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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AsCas12a

Plasmid

#188492

Purpose

Expresses FLAG tagged AsCas12a

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

Cas12a

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-CalfluxCTN

Plasmid

#190068

Purpose

Expresses BRET Ca2+ sensor CalfluxCTN in mammalian cells

Depositor

Carl Johnson

Article

Cumberbatch et al Sci Signal. 2022 Aug 16;15(747):eabq7618

Insert

CalfluxCTN

Expression

Mammalian

Promoter

CAG promoter

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pYL-kasOp-FnCas12a3

Plasmid

#177875

Purpose

Expresses Streptomyces codon optimized FnCas12a. Used to modify genome of Streptomyces.

Depositor

Yunzi Luo

Article

Zhang et al Front Bioeng Biotechnol. 2020 Jun 30;8:7

Insert

Streptomyces codon optimized the FnCas12a mutant EP16

Use

CRISPR

Promoter

kasOp*

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pSYC-437

Plasmid

#163829

Purpose

Foxj1a:GCaMP6s. Fluorescent reporter for calcium signaling (GCaMP6s) driven by zebrafish foxj1a promoter.

Depositor

Seok-Yong Choi

Article

Zhang et al Nat Commun. 2020 Apr 20;11(1):1860. doi:

Inserts

Foxj1a Promoter
GCaMP6s

Use

Multisite gateway destination vector

Available Since

Feb. 5, 2021

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-AIDmax-BlastR

Plasmid

#152994

Purpose

C-to-T base editor

Depositor

David Liu

Article

Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

Insert

AID-nSpCas9-UGI-UGI

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

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pSW071

Plasmid

#140037

Purpose

PCC 11901 fadD locus KO with a kanamycin resistance cassette

Depositor

Peter Nixon

Article

Wlodarczyk et al Commun Biol. 2020 May 7;3(1):215. doi: 1

Insert

Kanamycin resistance gene

Use

Gene knockout in cyanobacteria

Promoter

KanR

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2-PLK1-mCherry

Plasmid

#127154

Purpose

Expresses mCherry PLK1 in mammalian cells and zebrafish embryos

Depositor

Heidi Hehnly

Article

Colicino et al Mol Biol Cell. 2019 Jun 15;30(13):1598-1

Insert

PLK1-mCherry (PLK1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV IE94

Available Since

June 19, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-dDIO-vCre

Plasmid

#118029

Purpose

AAV vector for sparse and bright labeling in a cell-type specific manner

Depositor

Minmin Luo

Article

Lin et al Nat Methods. 2018 Nov 19. pii: 10.1038/s

Insert

TRE-dDIO-vCre

Use

AAV

Mutation

See depositor comments below

Promoter

TRE

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn5-135

Plasmid

#118521

Purpose

miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mScarlet-I

Use

Minitn5 delivery vector

Expression

Bacterial

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pQE80L TriCatcher-ELP

Plasmid

#112630

Purpose

Two SpyCatchers linked by an elastin-like polypeptide with a C-terminal SnoopCatcher

Depositor

Mark Howarth

Article

Wieduwild et al Biomaterials. 2018 Oct;180:253-264. doi:

Insert

TriCatcher-ELP

Tags

His6 and TEV cleavage site

Expression

Bacterial

Promoter

T5

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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VinculinTS-I997A

Plasmid

#111828

Purpose

Vinculin tension sensor with point mutation affecting ability to bind actin

Depositor

Brenton Hoffman

Article

Rothenberg et al Biophys J. 2018 Apr 10;114(7):1680-1694.

Insert

VinculinTS (I997A)

Expression

Mammalian

Mutation

changed isoleucine 997 to alanine

Promoter

CMV

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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pAB107a

Plasmid

#79218

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ-opt (OR1) gIII, luxAB; Ppro1 434cI

Expression

Bacterial

Promoter

PlacZ-opt (OR1); Ppro1

Available Since

May 12, 2018

Availability

Academic Institutions and Nonprofits only

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PX458_AHR_1

Plasmid

#101076

Purpose

Encodes gRNA for 3' target of human AHR

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against AHR (AHR Human)

Use

CRISPR

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pOTTC1329 - pAAV EF1a HA-hM4D(Gi)

Plasmid

#89150

Purpose

An AAV packaging plasmid that expresses HA-tagged hM4D(Gi) inhibitory DREADD from the EF1a promoter.

Depositor

Brandon Harvey

Article

Gomez et al Science. 2017 Aug 4;357(6350):503-507. d

Insert

hM4D(Gi)

Use

AAV

Tags

HA

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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GAG-Pan

Plasmid

#99640

Purpose

EGFP-mCherry Met71GAG reporter for methionine misincorporation

Depositor

Tao Pan

Article

Gomes et al RNA. 2016 Mar;22(3):467-76. doi: 10.1261

Insert

mCherry

Tags

EGFP

Expression

Mammalian

Mutation

Met71 mutated to GAG (Glu)

Promoter

CMV

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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GAA-Pan

Plasmid

#99639

Purpose

EGFP-mCherry Met71GAA reporter for methionine misincorporation

Depositor

Tao Pan

Article

Gomes et al RNA. 2016 Mar;22(3):467-76. doi: 10.1261

Insert

mCherry

Tags

EGFP

Expression

Mammalian

Mutation

Met71 mutated to GAA (Glu)

Promoter

CMV

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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