We narrowed to 14,379 results for: TIM

Showing: 10701 - 10720 of 14379 results

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PA-DreN

Plasmid

#169707

Purpose

The optimized DreN246-nMag

Depositor

Dali Li

Article

Li et al Proc Natl Acad Sci U S A. 2020 Dec 29;11

Insert

Dre246-nMag

Expression

Mammalian

Mutation

none

Promoter

CAG

Available Since

May 24, 2021

Availability

Academic Institutions and Nonprofits only

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PA-DreC

Plasmid

#169705

Purpose

The optimized pMag-Dre247

Depositor

Dali Li

Article

Li et al Proc Natl Acad Sci U S A. 2020 Dec 29;11

Insert

pMag-Dre247

Expression

Mammalian

Mutation

none

Promoter

CAG

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-HsSENP7

Plasmid

#71470

Purpose

Encodes human full length SENP7. Suitable for in vitro transcription/translation (T7).

Depositor

Jorge Eduardo Azevedo

Article

Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

Insert

SUMO1/sentrin specific peptidase 7 (SENP7 Human)

Tags

Hexahistidine

Expression

Bacterial

Mutation

Gene optimised for E. coli expression (Genscript)

Promoter

T7

Available Since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only
pXWJ109As-gfp

Plasmid

#123143

Purpose

An arsenic sensor with optimized intracellular receptor density. pSB3K3 carrying J109-30arsR-t-ParsR-30gfp-t

Depositor

Baojun Wang

Article

Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

Insert

J109-30arsR-t-ParsR-30gfp-t

Use

Synthetic Biology

Expression

Bacterial

Promoter

J109, ParsR

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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pVH72-Tier1-PhCMV-SEAP

Plasmid

#169560

Purpose

Tier-1 vector encoding PhCMV-driven SEAP expression containing a computationally optimized secretion peptide (PhCMV-SEAP-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven human secreted placental alkaline phosphatase

Expression

Mammalian

Promoter

PhCMV

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-parkin W403A

Plasmid

#45974

Depositor

Kalle Gehring

Article

Trempe et al Science. 2013 May 9.

Insert

Parkin W403A (Prkn Rat)

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression. Chan…

Promoter

tac

Available Since

Sept. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pCSN074

Plasmid

#166731

Purpose

Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS, codon optimized for Saccharomyces cerevisiae

Depositor

Rene Verwaal

Article

Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312

Insert

dCas12a-NLS

Use

CRISPR

Tags

SV40 NLS

Expression

Yeast

Mutation

E925A

Promoter

S. cerevisiae TEF1

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pfTRAP

Plasmid

#50048

Purpose

Expresses plasmodium falciparum TRAP adhesion domains (26-299) in mammalian cells

Depositor

Timothy Springer

Article

Song et al Proc Natl Acad Sci U S A. 2012 Dec 26;10

Insert

plamodium falciparum TRAP (TRAP Synthetic)

Tags

6Xhis tag

Expression

Mammalian

Mutation

C55G, N132S

Promoter

CMV

Available Since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only
pfTRAP(VWA)

Plasmid

#50049

Purpose

Expresses plasmodium falciparum TRAP VWA domain (41-240) in mammalian cells

Depositor

Timothy Springer

Article

Song et al Proc Natl Acad Sci U S A. 2012 Dec 26;10

Insert

pfTRAP(vwa) (TRAP Synthetic)

Tags

6Xhis tag

Expression

Mammalian

Mutation

C55G, N132S

Promoter

CMV

Available Since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only
pEBB-HA-hILP del RING

Plasmid

#25675

Depositor

Colin Duckett

Article

Lewis et al J Biol Chem. 2004 Mar 5. 279(10):9023-9.

Insert

XIAP (XIAP Human)

Tags

HA

Expression

Mammalian

Mutation

Encodes aa 1-449 only. aa 450-497 have been dele…

Available Since

Aug. 11, 2010

Availability

Academic Institutions and Nonprofits only
pMC-6-RLuc-9

Plasmid

#197739

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

Renilla luciferase (tomato optimized)

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-parkin C431S

Plasmid

#45976

Depositor

Kalle Gehring

Article

Trempe et al Science. 2013 May 9.

Insert

Parkin C431S (Prkn Rat)

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression. Chan…

Promoter

tac

Available Since

Sept. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pCXLE-dCas9GFP-shP53

Plasmid

#102906

Purpose

EBNA episome plasmid for CAG promoter constitutive expression of dCas9-GFP. Includes p53 shRNA expression cassette.

Depositor

Timo Otonkoski

Article

Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

Insert

dCas9GFP-shP53

Use

CRISPR

Expression

Mammalian

Mutation

D10A, H840A

Promoter

CAG

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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pALPS_3'LTR GFP@gag start SFFV rev

Plasmid

#101339

Purpose

LTR drives GFP and internal SFFV promoter drives codon optimized rev

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

GFP, rev

Use

Lentiviral

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pZyxin-mMaple2

Plasmid

#101150

Purpose

Fluorescent protein for superresolution imaging and a focal adhesion protein for binding substrate

Depositor

Xiaowei Zhuang

Article

Wang et al Proc Natl Acad Sci U S A. 2014 Jun 10;11

Insert

human codon optimized mMaple2 fused to zyxin

Expression

Mammalian

Promoter

cmv

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3_AKAR5Δ

Plasmid

#119912

Purpose

Mammalian expression of the AKAR5Δ sensor with N-terminal His tag for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

Depositor

Haining Zhong

Article

Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

Insert

AKAR5Δ

Tags

6xHis

Expression

Mammalian

Available Since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pNC1137

Plasmid

#41563

Depositor

Beverly Errede

Article

Houser et al Yeast. 2012 Dec;29(12):519-30. doi: 10.1

Insert

Ura3-FUS1-UBIMdkGFP*-SpHis5-Tim9

Mutation

GFP mutations: Phe 64 to Leu, S65 to Thr, V163 t…

Available Since

April 2, 2013

Availability

Academic Institutions and Nonprofits only

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pEG BacMam N term myc eGFP 3C

Plasmid

#160682

Purpose

Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

Depositor

Eric Gouaux

Article

Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

Type

Empty backbone

Tags

myc eGFP 3C

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pUPD2 SV40-AcrIIA4 (GB3333)

Plasmid

#160605

Purpose

SV40-AcrIIA4 codon optimized for N.benthamiana

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2022 Feb;20(2):399-4

Insert

AcrIIA4

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pGK415-phlTA2-1E

Plasmid

#165970

Purpose

Expresses phlTA(2-1E) mutant

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

Insert

phlF

Use

Synthetic Biology

Tags

Nuclear localization signal and three copies of V…

Expression

Yeast

Mutation

P5S, S6P, K86T, F109L, Q117R, E143K, codon-optimi…

Promoter

pPGK1 mutant

Available Since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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