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pFRT-TO-RPB1-His K1278R CRres si2,4R

Plasmid

#139406

Purpose

Dox-inducible, K1278R C-terminal His tagged RPB1, resistant to siRNAs 2 & 4, for generation of TRex flp In cells.

Depositor

Jesper Svejstrup

Article

Tufegdzic Vidakovic et al Cell. 2020 Mar 5. pii: S0092-8674(20)301

Insert

RPB1 (POLR2A Human)

Use

Tags

6xHis

Expression

Mammalian

Mutation

K1278R

Promoter

Available since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKD3

Plasmid

#170848

Purpose

AAV backbone for Flp-dependent transgene expression of any transgene in cortical interneurons under the control of the Dlx enhancer.

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Type

Empty backbone

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pFRT-TO-RPB1-His CRres K619,627,642R si2,4R

Plasmid

#139409

Purpose

Dox-inducible, K619, 627, 642R C-terminal His tagged RPB1, resistant to siRNAs 2 & 4, for generation of TRex flp In cells.

Depositor

Jesper Svejstrup

Article

Tufegdzic Vidakovic et al Cell. 2020 Mar 5. pii: S0092-8674(20)301

Insert

RPB1 (POLR2A Human)

Use

Tags

6xHis

Expression

Mammalian

Mutation

K619, 627, 642R

Promoter

Available since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pFRT-TO-RPB1-His K751,758,767R CRres si2,4R

Plasmid

#139408

Purpose

Dox-inducible, K751, 758, 767R C-terminal His tagged RPB1, resistant to siRNAs 2 & 4, for generation of TRex flp In cells.

Depositor

Jesper Svejstrup

Article

Tufegdzic Vidakovic et al Cell. 2020 Mar 5. pii: S0092-8674(20)301

Insert

RPB1 (POLR2A Human)

Use

Tags

6xHis

Expression

Mammalian

Mutation

K751, 758, 767R

Promoter

Available since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pFRT-TO-RPB1-His K710,719R CRres si2,4R

Plasmid

#139407

Purpose

Dox-inducible, K710, 719R C-terminal His tagged RPB1, resistant to siRNAs 2 & 4, for generation of TRex flp In cells.

Depositor

Jesper Svejstrup

Article

Tufegdzic Vidakovic et al Cell. 2020 Mar 5. pii: S0092-8674(20)301

Insert

RPB1 (POLR2A Human)

Use

Tags

6xHis

Expression

Mammalian

Mutation

K710,719R

Promoter

Available since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

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pFRT-TO-FLAG(N)-CDC73

Plasmid

#231751

Purpose

Expression in Flp-IN human cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Insert

CDC73 (CDC73 Human)

Use

Tags

Flag-3C

Expression

Mammalian

Mutation

siCDC73#6 and #8 resistant

Promoter

Available since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pFRT-TO-FLAG(N)-CDC73deltaKIM

Plasmid

#231752

Purpose

Expression in Flp-IN human cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Insert

CDC73 (CDC73 Human)

Use

Tags

Flag-3C

Expression

Mammalian

Mutation

siCDC73#6 and #8 resistant; CDC73(delta290-324)

Promoter

Available since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pFRT-TO-FLAG(N)-CDC73-YY/AA

Plasmid

#231753

Purpose

Expression in Flp-IN human cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Insert

CDC73 (CDC73 Human)

Use

Tags

Flag-3C

Expression

Mammalian

Mutation

siCDC73#6 and #8 resistant; CDC73(Y290A, Y293A)

Promoter

Available since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Con/Fon-EYFP

Plasmid

#231926

Purpose

Cre-on/Flp-on EYFP under the Ef1a promoter

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Insert

eYFP with introns

Use

AAV

Tags

Expression

Mutation

Promoter

Ef1a

Available since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Coff/Fon-EYFP

Plasmid

#231927

Purpose

Cre-off/Flp-on EYFP under the Ef1a promoter

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Insert

eYFP with introns

Use

AAV

Tags

Expression

Mutation

Promoter

Ef1a

Available since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-fDIO-ArgiNLS-AausFP1

Plasmid

#220605

Purpose

Flp-dependent expression of a single-cell discriminating version of AausFP1 fluorescent protein.

Depositor

Sam Golden

Article

Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

Insert

AausFP1

Use

AAV

Tags

ArgiNLS

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-fDIO-ArgiNLS-mKate2

Plasmid

#220607

Purpose

Flp-dependent expression of a single-cell discriminating version of mKate2 fluorescent protein.

Depositor

Sam Golden

Article

Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

Insert

mKate2

Use

AAV

Tags

ArgiNLS

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-fDIO-ArgiNLS-oScarlet

Plasmid

#220606

Purpose

Flp-dependent expression of a single-cell discriminating version of oScarlet fluorescent protein.

Depositor

Sam Golden

Article

Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

Insert

oScarlet

Use

AAV

Tags

ArgiNLS

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-miniTurbo-TAF5-FL

Plasmid

#209051

Purpose

Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of miniTurbo fused to full length TAF5 isoform

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Insert

TAF5 full length

Use

Tags

miniTurbo

Expression

Mammalian

Mutation

Promoter

CMV/TO inducible promoter

Available since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-3xFLAG-EGFP

Plasmid

#209058

Purpose

Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of 3xFlag-tagged EGFP

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Insert

EGFP

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV/TO inducible promoter

Available since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-miniTurbo-EGFP

Plasmid

#209059

Purpose

Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of miniTurbo fused to EGFP

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Insert

EGFP

Use

Tags

miniTurbo

Expression

Mammalian

Mutation

Promoter

CMV/TO inducible promoter

Available since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5-3xFLAG-TAF5-FL

Plasmid

#209049

Purpose

Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of 3xFlag-tagged full length TAF5 isoform

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Insert

TAF5 full length

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV/TO inducible promoter

Available since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pRD443

Plasmid

#168469

Purpose

For the insertion pf NLS-mScarlet-I-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex system

Depositor

Remus Dame

Article

Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

Insert

NLS-mScarlet-I-H-NSdbd-NLS

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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Ai65(RCFL-tdT) targeting vector

Plasmid

#61577

Purpose

Target a Flp and Cre-dependent tdTomato expression cassette to the mouse Rosa26 locus

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Insert

pCAG-FSF-LSL-tdTomato

Use

Cre/Lox and Mouse Targeting

Tags

Expression

Mutation

Promoter

CAG

Available since

June 30, 2015

Availability

Academic Institutions and Nonprofits only

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TIGRE docking site targeting vector

Plasmid

#61580

Purpose

Create a docking site in the mouse TIGRE genomic locus that allows modification in ES cells by Flp recombinase-mediated cassette exchange

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Type

Empty backbone

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

July 29, 2015

Availability

Academic Institutions and Nonprofits only

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