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Plasmid#216842PurposeN-term Flag-HA tagged Spirodela polyrhiza (Sp9509) AGO4a cDNA under regulation of CaMV 35S promoterDepositorInsertARGONAUTE4
UseTagsFlag-HAExpressionPlantMutationPromoterCaMV 35SAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-HA_aa 1-542 POLK
Plasmid#201585PurposeExpresses the N-terminal clasp domain and catalytic domain (aa 1-542) of human DNA polymerase kappa with a HA tag in mammalian cellsDepositorInsertPolymerase kappa (POLK Human)
UseTagsHAExpressionMammalianMutationExpresses amino acids 1-542 of DNA polymerase kap…PromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCCM
Plasmid#162708PurposeSecond CCM operon cloned from H. neapolitanus; pFA expressing the remaining 11 genes in the H. neapolitanus CCM clusterDepositorInsertSecond CCM operon cloned from H. neapolitanus
UseTagsExpressionBacterialMutationPromoterPLtet0-1 promoterAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
Linked VLP NES
Plasmid#182432PurposeYeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and fusion protein VP2C-ERG20-AcNES1 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertsVP2C-FPPS-NES
deltaVP1
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL1 and GAL10Available sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Linked VLP NES (M)
Plasmid#182434PurposeYIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and fusion protein VP2C-ERG20(F96W-N127W)-AcNES1 (GAL10 promoter). Contains uracil marker (K. lactis URA3).DepositorInsertsVP2C-FPPS(M)-NES
deltaVP1
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL1 and GAL10Available sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Linked VLP NES (G)
Plasmid#182476PurposeYIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and fusion protein VP2C-ERG20(F96C)-AcNES1 (GAL10 promoter). Contains uracil marker (K. lactis URA3).DepositorInsertsVP2C-FPPS(G)-NES
deltaVP1
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL1 and GAL10Available sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-TetOn-mCherry-OAZ1_FS-YFP
Plasmid#232356PurposeMammalian expression of dox-inducible polyamine sensor (polyamine levels = YFP/Cherry)DepositorInsertOAZ1 derived polyamine sensing module (OAZ1 Human)
UseLentiviralTagseYFP and mCherryExpressionMammalianMutationPromoterTRE3GAvailable sinceMarch 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
deltaVP1 + VP2C-GFP
Plasmid#166676PurposeYeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and GFP tagged with VP2C (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3)DepositorInsertsVP2C-GFP
Murine polyomavirus deltaVP1 (NLS deletion mutant)
UseSynthetic BiologyTagsExpressionYeastMutationPromoterGAL1 and GAL10Available sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
Coexpressed VLP NES
Plasmid#182433PurposeYeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter), VP2C-ERG20 (GAL10 promoter), and VP2C-AcNES1 (GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3)DepositorInsertsVP2C-FPPS
deltaVP1
VP2C-NES
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL1, GAL10, and GAL7Available sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Coexpressed VLP NES (M)
Plasmid#182435PurposeYIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter), VP2C-ERG20(F96W-N127W) (GAL10 promoter), and VP2C-AcNES1 (GAL7 promoter). Contains uracil marker (K. lactis URA3).DepositorInsertsVP2C-FPPS(M)
deltaVP1
VP2C-NES
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL1, GAL10, and GAL7Available sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Coexpressed VLP NES (G)
Plasmid#182477PurposeYIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter), VP2C-ERG20(F96C) (GAL10 promoter), and VP2C-AcNES1 (GAL7 promoter). Contains uracil marker (K. lactis URA3).DepositorInsertsVP2C-FPPS(G)
deltaVP1
VP2C-NES
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL1, GAL10, and GAL7Available sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
wtVP1 + VP2C-GFP
Plasmid#166674PurposeYeast integrative plasmid for expressing wild-type Murine polyomavirus VP1 (GAL1 promoter) and GFP tagged with VP2C (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertsVP2C-GFP
Wild-type Murine polyomavirus VP1
UseSynthetic BiologyTagsExpressionYeastMutationPromoterGAL1 and GAL10Available sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCOA-Leu-FvPPT1-fsr1
Plasmid#179868PurposeConstitutive expression vector for Saccharomyces cerevisiae comprising the polyketide synthase fsr1 from Fusarium vanettenii and the Fusarium verticillioides 4´-phosphopantetheinyltransferase PPT1DepositorInsertsPPT1
fsr1
UseTagsExpressionYeastMutationCodon optimized for expression in Saccharomyces c…PromoterYarrowia lipolytica EXP1 and Yarrowia lipolytica …Available sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCfb12567
Plasmid#219927PurposePrCYC with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALIDepositorInsertPromoter CYC
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCfb12566
Plasmid#219926PurposePrDGA with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALIDepositorInsertPromoter DGA
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCfb12622
Plasmid#219929PurposePrACT with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALIDepositorInsertPromoter ACT
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfb12562
Plasmid#219923PurposePrGPD with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALIDepositorInsertPromoter GPD
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfb13186
Plasmid#219924PurposePrTEF with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALIDepositorInsertPromoter TEF
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfb12620
Plasmid#219928PurposePrTPI with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALIDepositorInsertPromoter TPI
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfb12561
Plasmid#219922PurposePrPGM with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALIDepositorInsertPromoter PGM
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 7, 2024AvailabilityAcademic Institutions and Nonprofits only