-
Plasmid#71266PurposeEntry clone containing ER-localized TagRFP. Can be used to construct transcriptional reporters. For use in plants and compatible with the MultiSite Gateway systemDepositorInsertTagRFP
UseGatewayTagsER retention signal HDEL and signal peptide (ER)…ExpressionMutationPromoterAvailable sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
p221k-erTq2CFP
Plasmid#71264PurposeEntry clone containing ER-localized Turqoise2. Can be used to construct transcriptional reporters. For use in plants and compatible with the MultiSite Gateway system.DepositorInsertTq2CFP
UseGatewayTagsER retention signal HDEL and signal peptide (ER)…ExpressionMutationPromoterAvailable sinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pVRC8400-SARS-CoV-2-S2P-BA.4/5-AVI
Plasmid#192112PurposeExpresses SARS-CoV-2 S2P protein from the BA.4 and BA.5 variants with single chain Fc, HRV3C protease cleavage site, and AVI tagDepositorInsertSARS-CoV-2-S2P-BA.4/5 (S )
UseTagsAVI, HRV3C, and scFcExpressionMammalianMutationT19I, L24S, P25-, P26-, A27-, H69-, V70-, G142D, …PromoterAvailable sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTol2-elavl3-WHaloCaMP1a-EGFP
Plasmid#205311PurposeExpression of WHaloCaMP1a-EGFP in zebrafish from the elavl3 promoter (pan-neuronal expression)DepositorInsertWHaloCaMP1a-EGFP
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTol2-elavl3-WHaloCaMP1a
Plasmid#205312PurposeExpression of WHaloCaMP1a in zebrafish from the elavl3 promoter (pan-neuronal expression)DepositorInsertWHaloCaMP1a
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTSlb-wt
Plasmid#60728PurposeExpresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD.DepositorInsertsResidues 1-179 of split T7 RNAP
Residues 180-880 of split T7 RNAP
UseSynthetic BiologyTagsExpressionBacterialMutationResidues 1-180 of split T7 RNAP and Residues 180-…PromoterAvailable sinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
TTR C10S S85C K80D
Plasmid#184743Purposebacterial expression of human TTR mutantsDepositorInsertTTR (TTR Human)
UseTagsExpressionBacterialMutationC10S, S85C, K80DPromoterT7Available sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.0_mitoLAMA-G97 IRES ShadowG-mScarlet
Plasmid#130709PurposePlasmid encoding outer mitochondrial localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and ShadowG-mScarletDepositorInsertNTOM20-SNAP-Tag-LAMA-G97 IRES ShadowG-mScarlet
UseTagsNTOM20 and SNAP-TagExpressionMammalianMutationPromoterAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRSETa-Amrose v1
Plasmid#79537PurposepRSETa (Invitrogen) plasmid expressing Amrose variant 1DepositorInsertAmrose
UseTags6xHis, T7 epitope, and Xpress tagExpressionBacterialMutationPromoterT7Available sinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMDC32-FIT2
Plasmid#96990PurposeExpress mouse FIT2 gene in plants.DepositorInsertMmFIT2 (Fitm2 Mouse)
UseTagsExpressionPlantMutationPromoter35SAvailable sinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMZ828
Plasmid#155322PurposeConstitutive expression of PhyB(1-650)-VP16 in plantsDepositorInsertPCaMV35S-PhyB(1-650)-VP16-NLS-Tnos
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
p10xUAS-WHaloCaMP1a-EGFP
Plasmid#205314PurposeExpression of WHaloCaMP1a-EGFP in Drosophila using GAL4 driverDepositorInsertWHaloCaMP1a-EGFP
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSET-WHaloCaMP-eNOSpep-EGFP
Plasmid#205306PurposeExpression of WHaloCaMP-eNOSpep-EGFP fusion in E. coliDepositorInsertWHaloCaMP-eNOSpep-EGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
TTR C10S S85C G83R
Plasmid#184747Purposebacterial expression of human TTR mutantsDepositorInsertTTR (TTR Human)
UseTagsExpressionBacterialMutationC10S, S85C, G83RPromoterT7Available sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
TTR C10S S85C Y78F
Plasmid#184746Purposebacterial expression of human TTR mutantsDepositorInsertTTR (TTR Human)
UseTagsExpressionBacterialMutationC10S, S85C, Y78FPromoterT7Available sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
TTR C10S S85C A81T
Plasmid#184745Purposebacterial expression of human TTR mutantsDepositorInsertTTR (TTR Human)
UseTagsExpressionBacterialMutationC10S, S85C, A81TPromoterT7Available sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
TTR C10S S85C K80E
Plasmid#184744Purposebacterial expression of huamn TTR mutantsDepositorInsertTTR (TTR Human)
UseTagsExpressionBacterialMutationC10S, S85C, K80EPromoterT7Available sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
TTR C10S S85C I84S
Plasmid#184748Purposebacterial expression of human TTR mutantsDepositorInsertTTR (TTR Human)
UseTagsExpressionBacterialMutationC10S, S85C, I84SPromoterT7Available sinceJuly 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2TK-WW-EF1+EF2
Plasmid#18080DepositorInsertDystrophin (DMD Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceMay 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
GST-mDia1(FH1FH2DAD)-His
Plasmid#85822Purposecontains the formin homolgy domains: FH2 is required for nucleation, processive tracking of barbed-end of actin filaments ; FH1 permits enhancement of actin polymerization rate.DepositorInsertProtein diaphanous homolog 1 (Diaph1 Mouse)
UseTags8*His and GSTExpressionBacterialMutationPromotertacAvailable sinceJuly 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pIRES2_EGFP_KCNQ1
Plasmid#173161PurposeFor mammalian expression of KCNQ1 and creating variantsDepositorInsertKCNQ1 (KCNQ1 Human)
UseTagsIRES2-EGFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-hsRPA32 (MSW#497)
Plasmid#208068PurposeExpression of GFP-tagged hsRPA2 in human cellsDepositorInsertRPA2 (RPA2 Human)
UseTagsGFPExpressionMammalianMutationT88I- please see depositor commentsPromoterAvailable sinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-synapsin-WHaloCaMP1a-EGFP
Plasmid#205307PurposeProduction of AAV particles encoding WHaloCaMP1a-EGFP from the synapsin promoterDepositorInsertWHaloCaMP1a-EGFP
UseAAVTagsExpressionMutationPromoterAvailable sinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTSlb-N748D
Plasmid#60732PurposeExpresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD. C-terminal fragment has the point mutations N748D.DepositorInsertsResidues 1-179 of split T7 RNAP
Residues 180-880 of split T7 RNAP
UseSynthetic BiologyTagsExpressionBacterialMutationResidues 1-180 of split T7 RNAP and Residues 180-…PromoterAvailable sinceNov. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
-
pCVL Traffic Light Reporter 2.1 (VF2468 ZFN target) Ef1a
Plasmid#31483DepositorInsertTraffic Light Reporter 2.1 (V2468 zfn target) EF Puro
UseLentiviralTagsExpressionMutationTraffic Light 2.0 series have a +3 PEST sequence …PromoterAvailable sinceAug. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
p2R3a-nosT
Plasmid#71272PurposeEntry clone containing nosT. Necessary to complete the transcriptional reporters by providing the poly-A tail. For use in plants and compatible with the MultiSite Gateway systemDepositorInsertnopaline synthase terminator
UseGatewayTagsExpressionMutationPromoterAvailable sinceDec. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYLsgRNA-OsU3m/LacZ
Plasmid#66192Purposecloning of sgRNAs for expression in plantsDepositorTypeEmpty backboneUseCloning vectorTagsExpressionMutationPromoterOsU3m (SpeI site destroyed)Available sinceAug. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMT1-24X-NOG(CmYLCV)
Plasmid#202014PurposeT-DNA vector for expression of the two protein components of the MoonTag system (dCas9-24XGP41 and NbGP41P-sfGFP-VP64-GB1) under the control of the CmYLCV promoter.DepositorInsertsdCas9-24XGP41
NbGP41P-sfGFP-VP64-GB1
UseTagsGB1 and sfGFPExpressionPlantMutationPromoterCmYLCVAvailable sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII-WHaloCaMP1a-EGFP
Plasmid#205309PurposeProduction of AAV particles encoding WHaloCaMP1a-EGFP from the CaMKII promoterDepositorInsertWHaloCaMP1a-EGFP
UseAAVTagsExpressionMutationPromoterAvailable sinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only