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We narrowed to 10,790 results for: T7

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Showing: 2121 - 2160 of 10790 results

  1. pJK581

    Plasmid
    #71695
    Purpose
    Produces Acetobacter aceti 1023 thioredoxin with a C-terminal His6 tag and Cys35>Ser mutant (AaTrxAH6-C35S)
    Depositor
    T. J. Kappock
    Insert
    thioredoxin
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changes cysteine-35 to serine
    Promoter
    T7
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71695-plasmid-map-sequence-id-134492

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  2. pJK558

    Plasmid
    #71704
    Purpose
    Produces Acetobacter aceti 1023 thioredoxin reductase 1 with Cys138>Ser mutant (AaTrxB1-C138S)
    Depositor
    T. J. Kappock
    Insert
    thioredoxin reductase 1
    Expression
    Bacterial
    Mutation
    changes cysteine-138 to serine
    Promoter
    T7
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71704-plasmid-map-sequence-id-131735

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  3. pJK505

    Plasmid
    #71594
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe mutant (AaPurE1fww)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Expression
    Bacterial
    Mutation
    changes tryptophan-34 to phenylalanine, additiona…
    Promoter
    T7
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71594-plasmid-map-sequence-id-131669

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  4. pJK506

    Plasmid
    #71596
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp165>Phe mutant (AaPurE1wwf)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Expression
    Bacterial
    Mutation
    changes tryptophan-165 to phenylalanine, addition…
    Promoter
    T7
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71596-plasmid-map-sequence-id-131670

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  5. 4QNS

    Plasmid
    #61957
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PF08_0034:MAC04J-B11:C229648
    Tags
    MHHHHHHSSGRENLYFQG
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7-lacO
    Available Since
    March 2, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  6. 2PD0

    Plasmid
    #60120
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    Cp-PFA0230c:MS-C01:C9434
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available Since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  7. 2PLU

    Plasmid
    #60131
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    cgd2_4120:MAC01Y-E08:C33348
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available Since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  8. 4GM2

    Plasmid
    #60132
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PF14_0348:PF14_0348_dml-X03:C228094
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available Since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  9. 3TB2

    Plasmid
    #60141
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PY04285:2002-B10:C5937
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available Since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  10. 4JXM

    Plasmid
    #60085
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    RRP9:JMC061-H10:C224101 (RRP9 Human)
    Use
    Baculovirus expression
    Tags
    6xHis
    Mutation
    See comments
    Promoter
    T7
    Available Since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  11. 4HAE

    Plasmid
    #51315
    Depositor
    Cheryl Arrowsmith
    Insert
    4HAE (CDYL2 Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Truncation: See Comments
    Promoter
    T7
    Available Since
    Feb. 12, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  12. pDC10

    Plasmid
    #75077
    Purpose
    Produces Escherichia coli glycinamide ribonucleotide synthetase (H6EcPurD)
    Depositor
    T. J. Kappock, JoAnne Stubbe
    Insert
    phosphoribosylglycinamide synthetase (purD Escherichia coli)
    Tags
    His6
    Expression
    Bacterial
    Mutation
    adds unique SacII site at Thr95-Ala96 by a silent…
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    75077-plasmid-map-sequence-id-142310

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  13. OmpA3-PNGaseF-TEV-His6

    Plasmid
    #114274
    Purpose
    Amidase removing almost all N-linked oligosaccharides from glycoproteins. This PNGase F construct can have its hexa-his removed.
    Depositor
    Martin Caffrey
    Insert
    Peptide: N-glycosidase F
    Tags
    OmpA3 leader sequence (for periplasmic localisati…
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    114274-plasmid-map-sequence-id-218322

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  14. pIVTR(T7A)-NFIB

    Plasmid
    #172303
    Purpose
    In vitro transcription of NFIB
    Depositor
    Patrik Verstreken
    Insert
    NFIB (NFIB Human)
    Use
    Other
    Promoter
    T7 promoter A-initiating
    Available Since
    Aug. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    172303-plasmid-map-sequence-id-340431

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  15. POLG-pcDNA3.1

    Plasmid
    #191487
    Purpose
    Expresses human POLG gene on pcDNA3.1 backbone. POLG gene is cloned with BamHI and XbaI restriction sites.
    Depositor
    Hans Zempel
    Insert
    POLG (POLG Human)
    Tags
    V5 tag, 6X His
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    191487-plasmid-map-sequence-id-415364

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  16. JDS246

    Plasmid
    #43861
    Purpose
    Expresses mammalian codon optimized Cas9 nuclease with C-term 3X FLAG from CMV and T7 promoters
    Depositor
    Keith Joung
    Insert
    mammalian codon-optimized streptococcus pyogenes Cas9 - 3X Flag
    Use
    CRISPR
    Tags
    3X FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    43861-plasmid-map-sequence-id-59200

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  17. pE-SUMO CRYGS

    Plasmid
    #80753
    Purpose
    Expression of human gamma S-crystallin in E. coli
    Depositor
    Larry David
    Insert
    CRYGS (CRYGS Human)
    Tags
    6xHIS and Smt3 SUMO
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    80753-plasmid-map-sequence-id-153560

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  18. AARS_C2_pNIC-Bio3

    Plasmid
    #153047
    Purpose
    Expression construct for producing Avi tagged Alanyl-tRNA synthetase
    Depositor
    Susanne Gräslund
    Insert
    AARS (AARS Human)
    Tags
    Avi and His6-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    June 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    153047-plasmid-map-sequence-id-304401

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  19. pET-Impact-T4gp43(exo-)

    Plasmid
    #228202
    Purpose
    Expression in BL21(DE3) of 3' to 5' exonuclease-deficient T4 DNA polymerase D219A
    Depositor
    Stephen Benkovic
    Insert
    43
    Tags
    Sce VMA intein-chitin binding domain
    Expression
    Bacterial
    Mutation
    Mutated aspartate 219 to alanine (D219A), elimina…
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    228202-plasmid-map-sequence-id-453943

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  20. TBXTA-c026

    Plasmid
    #139763
    Purpose
    Protein expression/GST pull-down
    Depositor
    Opher Gileadi
    Insert
    TBXT, Full-length, WT (TBXT Human)
    Tags
    His6-GST-TEV
    Expression
    Bacterial
    Mutation
    Contains only amino acids S2- M435
    Promoter
    T7
    Available Since
    May 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    139763-plasmid-map-sequence-id-296576

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  21. AARS_C1_pNIC-Bio3

    Plasmid
    #153046
    Purpose
    Expression construct for producing Avi tagged Alanyl-tRNA synthetase
    Depositor
    Susanne Gräslund
    Insert
    AARS (AARS Human)
    Tags
    Avi and His6-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    June 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    153046-plasmid-map-sequence-id-304398

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  22. pGTVL2-CRBN-TBD

    Plasmid
    #196369
    Purpose
    protein expression of the GST-tagged Cereblon Thalidomide binding domain
    Depositor
    Stefan Knapp
    Insert
    CRBN (CRBN Human)
    Tags
    His6-GST-TEV
    Expression
    Bacterial
    Mutation
    contains only T319-T425
    Promoter
    T7
    Available Since
    March 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    196369-plasmid-map-sequence-id-385991

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  23. 9AVA

    Plasmid
    #220884
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    TREX1 (TREX1 Human)
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    June 20, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    220884-plasmid-map-sequence-id-436793

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  24. pmCellFree_KA1_SARS-COV-2_S

    Plasmid
    #169402
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus S with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _S (S SARS-Cov2)
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Promoter
    T7- CMV/CAG
    Available Since
    June 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    169402-plasmid-map-sequence-id-336167

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  26. pET28-MKH8SUMO

    Plasmid
    #79526
    Purpose
    Bacterial expression plasmid used for T7 promoter driven expression of recombinant proteins with N-terminal 8X His, thrombin cleavage site, SUMO, and TEV cleavage site.
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Tags
    8xHis-Thrombin-SUMO-TEV and TEV cleavage site
    Expression
    Bacterial
    Available Since
    June 16, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits

  27. pET28a_LYN

    Plasmid
    #224238
    Purpose
    Expression of human LYN Kinase in E. coli
    Depositor
    Andrew Mesecar
    Insert
    lyn (LYN Human)
    Tags
    6xHis and 6xHis-TEV
    Expression
    Bacterial
    Promoter
    T7 promotor
    Available Since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    224238-plasmid-map-sequence-id-458419

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  28. R619-X67-527: His6-SARS-CoV-2 N

    Plasmid
    #170204
    Purpose
    E. coli expression of SARS-CoV-2 nucleocapsid protein
    Depositor
    Dominic Esposito
    Insert
    SARS-CoV-2 N (N SARS-CoV-2)
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    170204-plasmid-map-sequence-id-334259

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  29. EYFP-Talin1-FERM

    Plasmid
    #110565
    Purpose
    bacterial expresion of EYFP-tagged Talin 1
    Depositor
    Tina Izard
    Insert
    Talin 1 (Tln1 Mouse)
    Tags
    (His)6
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    110565-plasmid-map-sequence-id-213906

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  30. pmCellFree_KA3_SARS-COV-2_RBD

    Plasmid
    #169405
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 RBD domain of S protein with C-term mCherry
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _RBD (S SARS-Cov2)
    Use
    Cell-free expression vector
    Tags
    mCherry
    Promoter
    T7- CMV/CAG
    Available Since
    July 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    169405-plasmid-map-sequence-id-336878

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  31. 4LG8

    Plasmid
    #60095
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PRPF19:JMC069-A01:C226630 (PRPF19 Human)
    Use
    Baculovirus expression
    Tags
    6xHis
    Mutation
    See comments
    Promoter
    T7
    Available Since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  33. DARS_pNIC-Bio3

    Plasmid
    #153048
    Purpose
    Expression construct for producing Avi tagged Glycyl-tRNA synthetase
    Depositor
    Susanne Gräslund
    Insert
    DARS (DARS1 Human)
    Tags
    Avi and His6-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    June 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    153048-plasmid-map-sequence-id-304402

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  34. SIRT6

    Plasmid
    #41565
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SIRT6 (SIRT6 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    aa 3-353
    Promoter
    T7
    Available Since
    April 9, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits

  35. pmCellFree_KA1_SARS-COV-2_ORF10

    Plasmid
    #169398
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus ORF10 with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _ORF10 (ORF10 SARS-Cov2)
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Promoter
    T7- CMV/CAG
    Available Since
    July 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    169398-plasmid-map-sequence-id-336872

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  36. pT7-7 asyn S87E/S129E

    Plasmid
    #36064
    Depositor
    Hilal Lashuel
    Insert
    alpha-synuclein S87E/S129E (SNCA Human)
    Expression
    Bacterial
    Mutation
    S87E/S129E
    Promoter
    T7
    Available Since
    July 13, 2012
    Availability
    Academic Institutions and Nonprofits only
    36064-plasmid-map-sequence-id-44521

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  38. NUDT4 (NUDT4A-c001)

    Plasmid
    #98241
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    NUDT4A (NUDT4 Human)
    Tags
    His-6-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Sept. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98241-plasmid-map-sequence-id-193419

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  39. pET MBP mClover LIC cloning vector (MBP-mClover)

    Plasmid
    #86930
    Purpose
    pET LIC cloning vector for Biotin-His6-MBP-N10-tev-yORF-mClover
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Tags
    6xHis, AviTag (biotin), MBP, TEV cleavage site, a…
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    86930-plasmid-map-sequence-id-174752

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  40. SMARCA2

    Plasmid
    #73250
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    SMARCA2B (SMARCA2 Human)
    Tags
    His-6-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    73250-plasmid-map-sequence-id-136215

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Showing: 2121 - 2160 of 10790 results