We narrowed to 10,596 results for: cat.1

1. pGS_N_Strep

   Plasmid

   #101651

   Purpose

   Expression of type I signal-peptide containing proteins with N-terminal Twin-Strep-tag in insect cells

   Depositor

   Bjoern Sander, Monika Golas

   Article

   Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

   Insert

   LRP6 Signal peptide, KpnI linker, Twin-Strep tag
   

   Use

   Tags

   Twin-Strep tag

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGS_C_Strep

   Plasmid

   #101652

   Purpose

   Expression of type I signal-peptide containing proteins with C-terminal Twin-Strep-tag in insect cells

   Depositor

   Bjoern Sander, Monika Golas

   Article

   Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

   Insert

   LRP6 Signal peptide, MCS linker, Twin-Strep tag
   

   Use

   Tags

   Twin-Strep tag

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pColE1_sgRNA_pos6_AmpR

   Plasmid

   #119149

   Purpose

   Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR

   Depositor

   Vincent Noireaux

   Article

   Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

   Insert

   sgRNA targeting position 6 in pColE1_70a_deGFP_KanR
   

   Use

   Crispr

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pYAL_HLA-A2_MART1

   Plasmid

   #171177

   Purpose

   Displays MART-1 peptide in HLA-A*02 as a single-chain trimer

   Depositor

   Michael Birnbaum

   Article

   Huisman et al Methods Mol Biol. 2022;2491:263-291. doi

   Insert

   MART1_HLA-A2*01 single-chain trimer fusion
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   GAL1

   Available since

   July 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pColE1_sgRNA_NT_AmpR

   Plasmid

   #119144

   Purpose

   Encodes non-targeting sgRNA

   Depositor

   Vincent Noireaux

   Article

   Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

   Insert

   non-targeting sgRNA
   

   Use

   Crispr

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pTNT-pT7-tetR-mCherry

   Plasmid

   #140870

   Purpose

   T7 promoter driven synthesis of TetR-mCherry fusion protein

   Depositor

   Neal Devaraj

   Article

   Niederholtmeyer et al Nat Commun. 2018 Nov 28;9(1):5027. doi:

   Insert

   TetR-mCherry expressed with T7 promoter
   

   Use

   Synthetic Biology

   Tags

   mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. vgCam-pRSETb

   Plasmid

   #193362

   Purpose

   A FRET-based calcium probe using Sumire

   Depositor

   Takeharu Nagai

   Article

   Sugiura et al Commun Biol. 2022 Nov 3;5(1):1172. doi:

   Insert

   vgCam
   

   Use

   E.coli expression

   Tags

   His X6 tag and Strep tag

   Expression

   Mutation

   Promoter

   T7

   Available since

   Feb. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMLS262

   Plasmid

   #73718

   Purpose

   C. elegans Pan-neuronal 2xNLS-FLP-D5 expression vector

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

   Insert

   Psnt-1::2xNLS-FLP-D5
   

   Use

   Tags

   Expression

   Worm

   Mutation

   G5D

   Promoter

   Psnt-1

   Available since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDJSVT91

   Plasmid

   #109305

   Purpose

   Express periplasmic, outer membrane, or secreted proteins in E. coli - SufI signal (TAT) and His6 tag

   Depositor

   Daniel Slade

   Article

   Casasanta et al Methods Mol Biol. 2019;1960:123-138. doi

   Type

   Empty backbone

   Use

   Tags

   SufI 1-30, 6His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pColE1_sgRNA_pos9_AmpR

    Plasmid

    #119152

    Purpose

    Encodes sgRNA targeting position 9 in pColE1_70a_deGFP_KanR

    Depositor

    Vincent Noireaux

    Article

    Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

    Insert

    sgRNA targeting position 9 in pColE1_70a_deGFP_KanR
    

    Use

    Crispr

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pColE1_sgRNA_pos8_AmpR

    Plasmid

    #119151

    Purpose

    Encodes sgRNA targeting position 8 in pColE1_70a_deGFP_KanR

    Depositor

    Vincent Noireaux

    Article

    Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

    Insert

    sgRNA targeting position 8 in pColE1_70a_deGFP_KanR
    

    Use

    Crispr

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pColE1_sgRNA_pos3_AmpR

    Plasmid

    #119146

    Purpose

    Encodes sgRNA targeting position 3 in pColE1_70a_deGFP_KanR

    Depositor

    Vincent Noireaux

    Article

    Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

    Insert

    sgRNA targeting position 3 in pColE1_70a_deGFP_KanR
    

    Use

    Crispr

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pColE1_sgRNA_pos2_AmpR

    Plasmid

    #119145

    Purpose

    Encodes sgRNA targeting position 2 in pColE1_70a_deGFP_KanR

    Depositor

    Vincent Noireaux

    Article

    Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

    Insert

    sgRNA targeting position 2 in pColE1_70a_deGFP_KanR
    

    Use

    Crispr

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Hox A11a

    Plasmid

    #36462

    Depositor

    Robert Ho

    Article

    Ahn et al Dev Biol. 2008 Oct 1;322(1):220-33. Epub

    Insert

    hoxa11a (hoxa11a Zebrafish)
    

    Use

    Riboprobe synthesis

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Sept. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pH6-2xmNeon

    Plasmid

    #178462

    Purpose

    A tandem mNeonGreen fluorescent protein (2xmNeon) with an N-terminal 6xHis-tag in pET28a.

    Depositor

    Siegfried Musser

    Article

    Sharma et al Commun Biol. 2022 Sep 19;5(1):988. doi:

    Insert

    mNeonGreen dimer
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTatABmNeonC

    Plasmid

    #178464

    Purpose

    pTatABC with an mNeonGreen fluorescent protein tag at the C-terminal end of TatB.

    Depositor

    Siegfried Musser

    Article

    Sharma et al Commun Biol. 2022 Sep 19;5(1):988. doi:

    Insert

    TatA, TatBmNeon, TatC
    

    Use

    Tags

    mNeonGreen fused to TatB

    Expression

    Bacterial

    Mutation

    Promoter

    araC

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTatABCmNeon

    Plasmid

    #178465

    Purpose

    pTatABC with an mNeonGreen fluorescent protein tag at the C-terminal end of TatC.

    Depositor

    Siegfried Musser

    Article

    Sharma et al Commun Biol. 2022 Sep 19;5(1):988. doi:

    Insert

    TatA, TatB, TatCmNeon
    

    Use

    Tags

    mNeonGreen fused to TatC

    Expression

    Bacterial

    Mutation

    Promoter

    araC

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. KIF3A560GFP

    Plasmid

    #129769

    Purpose

    Expresses mouse Kinesin-2 KIF3A head and neck linker, dimerized through kinesin-1 neck-coil and coil-1 to 560 and GFP tagged

    Depositor

    William Hancock

    Insert

    KIF3A (Kif3a Mouse)
    

    Use

    Tags

    GFP and His6

    Expression

    Bacterial

    Mutation

    Truncation at 359, fused to kinesin-1 starting Al…

    Promoter

    Available since

    Nov. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSCM167-Flag-hSFRP1(aa181-314)

    Plasmid

    #189551

    Purpose

    Protein expression of SFRP1 in mammalian cells. Contains only Netrin (NTR) domain--amino acids 181-314.

    Depositor

    Haian Fu

    Insert

    truncated secreted frizzled related protein 1 (SFRP1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    contains aa181-314

    Promoter

    Available since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.2-V5-DEST-hSFRP1(aa53-166)

    Plasmid

    #189544

    Purpose

    Protein expression of SFRP1 in mammalian cells. Contains only Frizzled cysteine rich domain (CRD)--amino acids 53-166.

    Depositor

    Haian Fu

    Insert

    truncated secreted frizzled related protein 1 (SFRP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    contains aa53-166

    Promoter

    Available since

    April 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pSCM167-Venus-VF-hSFRP1(aa181-314)

    Plasmid

    #189550

    Purpose

    Protein expression of SFRP1 in mammalian cells. Contains only Netrin (NTR) domain--amino acids 181-314.

    Depositor

    Haian Fu

    Insert

    truncated secreted frizzled related protein 1 (SFRP1 Human)
    

    Use

    Tags

    VF

    Expression

    Mammalian

    Mutation

    contains aa181-314

    Promoter

    Available since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pSCM167-Flag-hSFRP1(aa53-169)

    Plasmid

    #189547

    Purpose

    Protein expression of SFRP1 in mammalian cells. Contains only Frizzled cysteine rich domain (CRD)--amino acids 53-169.

    Depositor

    Haian Fu

    Insert

    truncated secreted frizzled related protein 1 (SFRP1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    contains aa53-169

    Promoter

    Available since

    Oct. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. TFORF2692

    Plasmid

    #142358

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    TEAD1 (TEAD1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. TFORF2060

    Plasmid

    #141899

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NKX2-1 (NKX2-1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. TFORF2470

    Plasmid

    #142097

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    PDX1 (PDX1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. TFORF2061

    Plasmid

    #141900

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NKX2-1 (NKX2-1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. TFORF1953

    Plasmid

    #142897

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    PARP1 (PARP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pOz-humBirA-GFP

    Plasmid

    #133283

    Purpose

    Expression of BirA-GFP fusions in mammalian cells

    Depositor

    Arman Kulyyassov

    Article

    Kulyyassov et al Biomolecules. 2020 Jan 16;10(1). pii: bi

    Insert

    eGFP
    

    Use

    Retroviral

    Tags

    Humanized wild type biotin ligase BirA

    Expression

    Mammalian

    Mutation

    Promoter

    MoMuLV

    Available since

    Jan. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Launchpad (AVA2590)

    Plasmid

    #85442

    Purpose

    Lentiviral FRT-flanked EF1alfa-GFP PGK-PuromycinR cassette

    Depositor

    Joan Steitz

    Article

    Alexandrov et al Mol Cell. 2017 Jan 5;65(1):191-201. doi:

    Insert

    FRT-flanked EF1alfa-GFP PGK-Puromycin cassette
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

30. pET28a-hdNadV

    Plasmid

    #83362

    Purpose

    Expresses nadV from Haemophilus ducreyi in Escherichia coli

    Depositor

    George Cătălin Marinescu

    Article

    Marinescu et al Sci Rep. 2018 Mar 13;8(1):4433. doi: 10.

    Insert

    nadV
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pSP64-AGS-mCherry

    Plasmid

    #135596

    Purpose

    Expression of AGS-mCherry mRNA for microinjection for visualization of AGS protein dynamics in S. purpuratus

    Depositor

    Mamiko Yajima

    Article

    Poon et al Nat Commun. 2019 Aug 22;10(1):3779. doi:

    Insert

    AGS-mCherry
    

    Use

    In vitro transcription (mrna synthesis)

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Unknown

    Available since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. membrane-HsPLK1

    Plasmid

    #185571

    Purpose

    to see protein dynamics

    Depositor

    Mamiko Yajima

    Article

    Poon et al Nat Commun. 2019 Aug 22;10(1):3779. doi:

    Insert

    PLK1 (PLK1 Human)
    

    Use

    In vitro transcription (mrna synthesis)

    Tags

    membrane targeting sequence from Neuromodulin (ML…

    Expression

    Mutation

    Promoter

    Available since

    July 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pH6-mNeonC

    Plasmid

    #178461

    Purpose

    The mNeonGreen fluorescent protein with an N-terminal 6xHis and a C-terminal free-Cysteine in pET28a

    Depositor

    Siegfried Musser

    Article

    Sharma et al Commun Biol. 2022 Sep 19;5(1):988. doi:

    Insert

    mNeonGreen
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Free cysteine at the C-terminus

    Promoter

    T7

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLL5.0 shCAV1-NLS-mCherry

    Plasmid

    #187257

    Purpose

    Lentiviral expression of shRNA targeting Caveolin1 and NLS-mCherry

    Depositor

    Alpha Yap

    Article

    Katsuno-Kambe et al Biol Cell. 2021 Feb;113(2):107-117. doi:

    Insert

    ShRNA for Caveolin-1 (Cav1 Mouse)
    

    Use

    Lentiviral

    Tags

    NLS-mCherry

    Expression

    Mutation

    Promoter

    U6

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNA3.2-V5-DEST-hSFRP1(aa181-314)-his

    Plasmid

    #189549

    Purpose

    Protein expression of SFRP1 in mammalian cells. Contains only Netrin (NTR) domain--amino acids 181-314.

    Depositor

    Haian Fu

    Insert

    truncated secreted frizzled related protein 1 (SFRP1 Human)
    

    Use

    Tags

    his

    Expression

    Mammalian

    Mutation

    contains aa181-314

    Promoter

    Available since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCherryGFP-CoV2-Inframe-stop

    Plasmid

    #177620

    Purpose

    Dual fluorescent protein reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. GFP is in-frame with mCherry. Thus GFP translation is prevented by stop codon within FSE (negative control).

    Depositor

    Junjie Guo

    Article

    Sun et al Proc Natl Acad Sci U S A. 2021 Jun 29;11

    Insert

    SARS-CoV-2 FSE
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pDEST17-His10-Smt3-DDX5ΔPrD(kf)-SNAP

    Plasmid

    #166150

    Purpose

    Expression of N. furzeri DDX5ΔPrD(1-535) mutant in E. coli

    Depositor

    Daniel Jarosz

    Article

    Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

    Insert

    DDX5ΔPrD(1-535)
    

    Use

    Tags

    His10-Smt3 and SNAP

    Expression

    Bacterial

    Mutation

    ΔPrD(1-535)

    Promoter

    T7

    Available since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pDEST17-His10-Smt3-DDX5ΔIDR(kf)-SNAP

    Plasmid

    #166151

    Purpose

    Expression of N. furzeri DDX5ΔIDR(1-483) mutant in E. coli

    Depositor

    Daniel Jarosz

    Article

    Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

    Insert

    DDX5ΔIDR(1-483)
    

    Use

    Tags

    His10-Smt3 and SNAP

    Expression

    Bacterial

    Mutation

    ΔIDR(1-483)

    Promoter

    T7

    Available since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. AAV.CBA.YFP.miR-E_shStk16-3

    Plasmid

    #180393

    Purpose

    Producing AAV that encodes mouse Stk16 shRNA-2 with miR-E backbone

    Depositor

    Huda Zoghbi

    Article

    Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

    Insert

    shStk16-3 (Stk16 Mouse)
    

    Use

    AAV and RNAi

    Tags

    Expression

    Mutation

    Promoter

    CBA

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pTT5-hSDC1

    Plasmid

    #52326

    Purpose

    expresses human syndecan 1 in HEK293-EBNA1 (293E) suspension culture.

    Depositor

    Gordon Laurie

    Article

    Zhang et al J Biol Chem. 2013 Apr 26;288(17):12090-1

    Insert

    SDC1 (SDC1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R117Q mutation (R95Q according to numbering in Fi…

    Promoter

    CMV

    Available since

    Dec. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge