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pSB1C3-J23110-B0034-mRFP1_Pink

Plasmid

#160445

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1_Pink chromoprotein in E. coli

Depositor

Anthony Forster

Article

Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

Insert

promoter, RBS, mRFP1E_Pink

Use

Synthetic Biology

Mutation

BioBrick sites removed

Available Since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc-p110α-CAAX

Plasmid

#39490

Depositor

Julian Downward

Article

Rodriguez-Viciana et al EMBO J. 1996 May 15;15(10):2442-51.

Insert

p110 (PIK3CA Bovine)

Tags

CAAX and myc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 21, 2012

Availability

Academic Institutions and Nonprofits only
pSB1C3-J23110-B0034-mRFP1_Orange

Plasmid

#160444

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1_Orange chromoprotein in E. coli

Depositor

Anthony Forster

Article

Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

Insert

promoter, RBS, mRFP1E_Orange

Use

Synthetic Biology

Mutation

BioBrick sites removed

Available Since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pLNCX Myr His p110 KD

Plasmid

#10837

Depositor

Thomas Roberts

Article

Auger et al Biochem Biophys Res Commun. 2000 Jun 16.

Insert

p110 KD (PIK3CA Bovine)

Use

Retroviral

Tags

6xHis and Myr

Expression

Mammalian

Mutation

D915S. Kinase Dead.

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
pSG5-Myr-FLAG-p110α-DM

Plasmid

#55713

Purpose

eukaryotic expression of myristoylation tag (Myr-FLAG) p110a RBD double mutant T207D, K227A

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

p110 alpha (Pik3ca Mouse)

Tags

myristoylation tag (Myr-FLAG)

Expression

Mammalian

Mutation

T207D, K227A

Promoter

T7

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
pRC/CMV-TYK2P1104A-VSV

Plasmid

#139350

Purpose

Expression of the TYK2-P1104A variant, rs34536443

Depositor

Sandra Pellegrini

Article

Li et al J Immunol. 2013 Mar 1;190(5):2335-44. do

Insert

TYK2 cDNA with 267nt of 5' UTR (TYK2 Human)

Tags

VSV

Expression

Mammalian

Mutation

V362F-P1104A

Promoter

CMV

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pEGB2Ω2_SF-35s:hCas9:tNos (GB1103)

Plasmid

#75400

Purpose

Transcriptional unit for (human codon optimized) Cas9 plant expression driven by the 35S promoter. Specially conceived to be linked with the TU of the kanamycin resistance gene GB1181.

Depositor

Diego Orzaez

Article

Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

Insert

hCas9

Use

CRISPR and Synthetic Biology

Expression

Plant

Promoter

35S

Available Since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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CAHS2parrc-mCh-2xFKBP (pBS1109)

Plasmid

#185304

Purpose

For the mammalian expression of the tardigrade protein CAHS2parrc attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

CAHS2parrc

Expression

Mammalian

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pLNCX Myr His p110

Plasmid

#10836

Depositor

Thomas Roberts

Article

Auger et al Biochem Biophys Res Commun. 2000 Jun 16.

Insert

p110 (PIK3CA Bovine)

Use

Retroviral

Tags

6xHis and Myr

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
R777-E110 Hs.IRS1-nostop

Plasmid

#70394

Purpose

Gateway ORF clone of human IRS1 [NM_005544.2] without stop codon (for C-terminal fusions)

Depositor

Dominic Esposito

Insert

IRS1 (IRS1 Human)

Use

Gateway entry clone

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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p6110 pSG5-HPV31-E8^E2C(HA)

Plasmid

#24741

Depositor

Peter Howley

Article

Powell et al J Virol. 2010 May;84(9):4451-60. doi: 10

Insert

HPV31 E8^E2C (HA)

Tags

HA tag in hinge region

Expression

Mammalian

Available Since

June 30, 2010

Availability

Academic Institutions and Nonprofits only
pBABE-zeo small T 110

Plasmid

#9063

Depositor

Bob Weinberg

Article

Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

Insert

SV40 small T Antigen 110 (SV40gp7 simian virus)

Use

Retroviral

Expression

Mammalian

Mutation

Contains only the N-termial 110 amino acids

Available Since

Sept. 23, 2005

Availability

Academic Institutions and Nonprofits only
pSG5-Myr-FLAG-p110β-DM

Plasmid

#55719

Purpose

eukaryotic expression of myristoylation tag (Myr-FLAG) p110b RBD double mutant S205D, K224A

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

p110 beta

Tags

myristoylation tag (Myr-FLAG)

Expression

Mammalian

Mutation

S205D, K224A

Promoter

T7

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA4/HisMaxB-YAP1-T110A

Plasmid

#18996

Depositor

Marius Sudol

Article

Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

Insert

Yes-kinase associated protein T110A mutant (YAP1 Human)

Tags

His

Expression

Mammalian

Mutation

Threonine 110 changed to Alanine

Available Since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
pLenti6/V5-DEST-p110 CUX1

Plasmid

#100816

Purpose

Gateway lentiviral vector expressing p100 CUX1 (amino acids 747-1505 of P39880), with an N-terminal Myc tag and a C-terminal HA tag

Depositor

Alain Nepveu

Article

Vadnais et al BMC Genomics. 2013 Apr 16;14:258. doi: 1

Insert

Hs CUX1 amino acids 747-1505 of P39880 (CUX1 Human)

Use

Lentiviral

Tags

HA and myc

Expression

Mammalian

Mutation

contains amino acids 747-1505

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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pSG5-myc-p110α-CAAX

Plasmid

#39494

Depositor

Julian Downward

Article

Rodriguez-Viciana et al EMBO J. 1996 May 15;15(10):2442-51.

Insert

p110 (PIK3CA Bovine)

Use

Eukaryotic

Tags

CAAX and myc

Available Since

Aug. 30, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3-myc-p110α(K227E)

Plasmid

#39491

Depositor

Julian Downward

Article

Rodriguez-Viciana et al EMBO J. 1996 May 15;15(10):2442-51.

Insert

p110(K227E) (PIK3CA Bovine)

Tags

myc

Expression

Mammalian

Mutation

K227E

Promoter

CMV

Available Since

Oct. 26, 2012

Availability

Academic Institutions and Nonprofits only
pGEX-Chapsyn-110-PDZ-2

Plasmid

#250074

Purpose

Bacterial expression of Chapsyn-110 PDZ domain 2

Depositor

Mark Bedford

Article

Espejo et al J Biol Chem. 2017 Feb 10;292(6):2255-226

Insert

Chapsyn-110 (2/3) (Dlg2 Mouse)

Expression

Bacterial

Mutation

None

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pGEX-Chapsyn-110-PDZ-3

Plasmid

#250075

Purpose

Bacterial expression of Chapsyn-110 PDZ domain 3

Depositor

Mark Bedford

Article

Espejo et al J Biol Chem. 2017 Feb 10;292(6):2255-226

Insert

Chapsyn-110 (3/3) (Dlg2 Mouse)

Expression

Bacterial

Mutation

None

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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SFFV-SP110-Brd 146

Plasmid

#219110

Purpose

Transcription factor SP110 with a specific barcode assigned.

Depositor

Filipe Pereira

Article

Kurochkin et al Cell Syst. 2026 Jan 21;17(1):101457. doi

Insert

SP110 (SP110 Human)

Use

Lentiviral

Promoter

The spleen focus-forming virus (SFFV) promoter

Available Since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

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