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pMXS-IRES-Blast CG1358

Plasmid

#218541

Purpose

Drosophila CG1358 cDNA (human codon optimized)

Depositor

Kivanc Birsoy

Article

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

CG1358 (HisT Fly)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast SLC5A7

Plasmid

#218539

Purpose

human SLC5A7 cDNA

Depositor

Kivanc Birsoy

Article

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

SLC5A7 (SLC5A7 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast SLC44A1

Plasmid

#218538

Purpose

human SLC44A1 cDNA

Depositor

Kivanc Birsoy

Article

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

SLC44A1 (SLC44A1 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast FLVCR1b

Plasmid

#218535

Purpose

human FLVCR1b cDNA

Depositor

Kivanc Birsoy

Article

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

FLVCR1 (FLVCR1 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Alternative isoform

Promoter

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pCW57-Cx32D178Y-IRES-GCaMP6s

Plasmid

#216796

Purpose

Inducible bicistronic lentiviral plasmid for the simultaneous expression of the D178Y mutant form of Connexin 32 and cytosolic GCaMP6s

Depositor

Fabio Mammano

Article

Tettey-Matey et al Cell Commun Signal. 2024 Dec 5;22(1):589

Insert

Cx32D178Y-IRES-GCaMP6s (GJB1 Human)

Use

Lentiviral

Tags

Expression

Mutation

D178Y mutant form of Connexin 32

Promoter

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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Golgi-FAST-IRES-iRFP670

Plasmid

#216345

Purpose

For testing localization of pFAST and iRFP670 proteins to Golgi complex

Depositor

Alison Tebo

Insert

ST6Gal1_pFAST-IRES-Sys1_iRFP670

Use

Tags

myc for FAST and iRF670 proteins

Expression

Mammalian

Mutation

n/a

Promoter

CMV

Available since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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Peroxisomes-FAST-IRES-iRFP670

Plasmid

#216347

Purpose

For testing localization of pFAST and iRFP670 proteins to peroxisomes

Depositor

Alison Tebo

Insert

pFAST_SKL-IRES-iRFP670_PXMP2

Use

Tags

myc for FAST protein only

Expression

Mammalian

Mutation

n/a

Promoter

CMV

Available since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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Mito-FAST-IRES-iRFP670

Plasmid

#216346

Purpose

For testing localization of pFAST and iRFP670 proteins to mitochondria

Depositor

Alison Tebo

Insert

Cox8_pFAST-IRES-Tomm20_iRFP670

Use

Tags

myc for FAST and iRF670 proteins

Expression

Mammalian

Mutation

n/a

Promoter

CMV

Available since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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pDonor-Prf1-ires-mCherry

Plasmid

#209073

Purpose

Targeting vector for the mouse Prf1 locus to correct prf1 gene

Depositor

Klaus Rajewsky

Article

Li et al Sci Immunol. 2024 Feb 2;9(92):eadi0042.

Insert

Prf1-ires-mCherry HDR template (parts of Prf1 with mutated PAM) (Prf1 Mouse)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 15, 2024

Availability

Academic Institutions and Nonprofits only

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LV-hGFAP-DLX2-ires-GFP

Plasmid

#213602

Purpose

Expression of human DLX2 and GFP under the human GFAP ABC1D promoter

Depositor

Chun-Li Zhang

Article

Zhang et al Proc Natl Acad Sci U S A. 2022 Mar 15;11

Insert

DLX2 (DLX2 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pVen-RPA32-IRES-RPA14

Plasmid

#211429

Purpose

mammalian expression of human RPA32 N-terminally fused to mVenus and RPA14

Depositor

Helmut Pospiech

Insert

RPA32 - IRES - RPA14 (RPA2, RPA3 Human)

Use

Tags

mVenus (on RPA32)

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-SUMO-A'-l2-A'-IRES-EmGFP

Plasmid

#209125

Purpose

Lentiviral vector including gene for CC-GEMS ligand SUMO-A'-l2-A' and EmGFP

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

SUMO-A'-l2-A'-IRES-EmGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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G1095C_BattB(GA)_jGCaMP7c_IRES_shBleR-P2A-HygroR

Plasmid

#200638

Purpose

jGCaMP7c recombination plasmid for Matreyek Bxb1(GA) landing pad in dual landing pad cells

Depositor

Kenneth Matreyek

Article

Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

Insert

jGCaMP7c

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pNK005C_BattB_mkozak_STIM1_IRES_mCherry-H2A-P2A-PuroR

Plasmid

#200639

Purpose

Low STIM1 abundance recombination plasmid for Matreyek Bxb1(GT) landing pad in dual landing pad cells

Depositor

Kenneth Matreyek

Article

Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

Insert

STIM1 (STIM1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pFSynW Syt9 IRES mRuby3

Plasmid

#195700

Purpose

Lentiviral plasmid encoding full-length mouse SYT9 followed by an internal ribosomal entry site followed by mRuby under the human synapsin promoter

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt9 (Syt5 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

encodes only the first 68 amino acids of Tac1

Promoter

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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SiRES COR1C DCC-GFP

Plasmid

#195149

Purpose

image COR1C

Depositor

Gia Voeltz

Article

Striepen et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

Insert

CORO1C (CORO1C Human)

Use

Tags

Expression

Mammalian

Mutation

mutations to make si RNA resistant and remove coi…

Promoter

Available since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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SiRES COR1C DCC -Halo

Plasmid

#195150

Purpose

image COR1C

Depositor

Gia Voeltz

Article

Striepen et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

Insert

CORO1C (CORO1C Human)

Use

Tags

Expression

Mammalian

Mutation

mutations to make si RNA resistant and remove coi…

Promoter

Available since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
SiRES COR1C ACT--GFP

Plasmid

#195151

Purpose

image COR1C

Depositor

Gia Voeltz

Article

Striepen et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

Insert

CORO1C (CORO1C Human)

Use

Tags

Expression

Mammalian

Mutation

mutations to make si RNA resistant and remove act…

Promoter

Available since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
SiRES COR1C ACT--Halo

Plasmid

#195152

Purpose

image COR1C

Depositor

Gia Voeltz

Article

Striepen et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

Insert

CORO1C (CORO1C Human)

Use

Tags

Expression

Mammalian

Mutation

mutations to make si RNA resistant and remove act…

Promoter

Available since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pCW57-Cx26G45E-IRES-GCaMP6s

Plasmid

#188237

Purpose

3rd generation, inducible bicistronic lentiviral plasmid for expression of human connexin 26 variant p.G45E and GCaMP6s

Depositor

Fabio Mammano

Article

Nardin et al Int J Mol Sci. 2022 Jun 30;23(13). pii:

Insert

GJB2 (GJB2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

p.G45E

Promoter

Tight TRE promoter

Available since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

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