We narrowed to 5,293 results for: cycs

1. pMT3-RhoGC E254D

   Plasmid

   #100033

   Purpose

   This plasmid is for expression of a rhodopsin/guanylyl cyclase fusion protein mutant with lambda max = 390 nm.

   Depositor

   Daniel Oprian

   Article

   Trieu et al J Biol Chem. 2017 Jun 23;292(25):10379-1

   Insert

   RhoGC E254D
   

   Use

   Tags

   1D4 (TETSQVAPA)

   Expression

   Mammalian

   Mutation

   E254D

   Promoter

   Adenovirus major late promoter

   Available since

   Sept. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

2. pGG-PIP50

   Plasmid

   #196788

   Purpose

   Contains ACS2 promoter

   Depositor

   Marc Somssich

   Article

   Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

   Insert

   1-AMINO-CYCLOPROPANE-1-CARBOXYLATE SYNTHASE 2 (ACS2 Mustard Weed)
   

   Use

   Greengate (gg) entry vector

   Tags

   Expression

   Mutation

   150 C->G

   Promoter

   Available since

   Dec. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTRE3G-NS3/4A S139A

   Plasmid

   #186885

   Purpose

   Doxycycline-dependent expression of catalytically inactive NS3/4A protease gene (S140A) in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

   Insert

   HCV NS3/4A protease with S139A mutation
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   S139A

   Promoter

   TRE3G

   Available since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTRE3G-WHG cGAS-HA

   Plasmid

   #186867

   Purpose

   Doxycycline-dependent expression of white-handed gibbon cGAS gene in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

   Insert

   White-handed gibbon cGAS with C-terminal HA tag
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mutation

   Promoter

   TRE3G

   Available since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAf-CRISPR-ctfR1

   Plasmid

   #207992

   Purpose

   CRISPR vector used with pAf-CRISPR-phoA (#207991) to delete both aflatoxin and cyclopiazonic acid gene clusters of Aspergillus flavus

   Depositor

   Perng-Kuang Chang

   Article

   Chang et al Fungal Genet Biol. 2023 Dec 27;170:10386

   Insert

   ctfR1
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Aspergillus flavus U6

   Available since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJ414-PCY1:S495A

   Plasmid

   #98151

   Purpose

   Expresses PCY1:S495A from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.

   Depositor

   James Naismith

   Article

   Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

   Insert

   Peptide cyclase 1:S495A
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   Changed serine 495 to alanine

   Promoter

   T7

   Available since

   March 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJ414-PCY1:W603A

   Plasmid

   #98153

   Purpose

   Expresses PCY1:W603A from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.

   Depositor

   James Naismith

   Article

   Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

   Insert

   Peptide cyclase 1:W603A
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   Changed tryptophan 603 to alanine

   Promoter

   T7

   Available since

   March 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJ414-PCY1:H695Q

   Plasmid

   #98154

   Purpose

   Expresses PCY1:H695Q from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.

   Depositor

   James Naismith

   Article

   Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

   Insert

   Peptide cyclase 1:H695Q
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   Changed histidine 695 to glutamine

   Promoter

   T7

   Available since

   March 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJ414-PCY1:H695A

   Plasmid

   #98155

   Purpose

   Expresses PCY1H695A from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.

   Depositor

   James Naismith

   Article

   Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

   Insert

   Peptide cyclase 1:H695A
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   Changed histidine 695 to alanine

   Promoter

   T7

   Available since

   March 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJ414-PCY1:Y696G

    Plasmid

    #98156

    Purpose

    Expresses PCY1:R696G from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.

    Depositor

    James Naismith

    Article

    Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

    Insert

    Peptide cyclase 1:Y696G
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    Changed tyrosine 696 to glycine

    Promoter

    T7

    Available since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMT3-RhoGC D380E

    Plasmid

    #100035

    Purpose

    This plasmid is for expression of a rhodopsin/guanylyl cyclase fusion protein mutant with lambda max = 533 nm.

    Depositor

    Daniel Oprian

    Article

    Trieu et al J Biol Chem. 2017 Jun 23;292(25):10379-1

    Insert

    RhoGC D380E
    

    Use

    Tags

    1D4 (TETSQVAPA)

    Expression

    Mammalian

    Mutation

    D380E

    Promoter

    Adenovirus major late promoter

    Available since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMT3-RhoGC D380N

    Plasmid

    #100034

    Purpose

    This plasmid is for expression of a rhodopsin/guanylyl cyclase fusion protein mutant with lambda max = 506 nm.

    Depositor

    Daniel Oprian

    Article

    Trieu et al J Biol Chem. 2017 Jun 23;292(25):10379-1

    Insert

    RhoGC D380N
    

    Use

    Tags

    1D4 (TETSQVAPA)

    Expression

    Mammalian

    Mutation

    D380N

    Promoter

    Adenovirus major late promoter

    Available since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBOB-EF1-FastFUCCI-Puro

    Plasmid

    #86849

    Purpose

    3rd generation lentiviral vector encoding FUCCI reporters for cell cycle analysis and puro for selection

    Depositor

    Duncan Jodrell, Kevin Brindle

    Article

    Koh et al J Cell Sci. 2016 Nov 25. pii: jcs.195164

    Inserts

    mKO2-hCDT1(30-120)
    mAG-hGEM(1-110)
    pac (puromycin N-acetyl-transferase)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1 and PGK

    Available since

    Nov. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLIB-GST-3C-CDK1

    Plasmid

    #177011

    Purpose

    Generate baculovirus for insect cell expression of CDK1 with N-terminal GST-3C (PreScission)

    Depositor

    Andrea Musacchio

    Article

    Huis In 't Veld et al Protein Sci. 2021 Nov 17. doi: 10.1002/p

    Insert

    CDK1 (CDK1 Human)
    

    Use

    Tags

    GST-3C

    Expression

    Insect

    Mutation

    codon-optimised for insect cell expression

    Promoter

    Available since

    Nov. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-sACt-mCherry-NLS

    Plasmid

    #138214

    Purpose

    Encodes residues 1-469 of rat soluble adenylyl cyclase (ADCY10) fused to mCherry; nuclear targeted.

    Depositor

    Jin Zhang

    Article

    Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.

    Insert

    sACt-mCherry-NLS (Adcy10 Rat)
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-sACt-mCherry-NES

    Plasmid

    #138215

    Purpose

    Encodes residues 1-469 of rat soluble adenylyl cyclase (ADCY10) fused to mCherry; cytosol targeted.

    Depositor

    Jin Zhang

    Article

    Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.

    Insert

    sACt-mCherry-NES (Adcy10 Rat)
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLentiDP1.0

    Plasmid

    #171094

    Purpose

    lentiviral construct (3rd generation) for the expression of cell cycle-independent OsTIR1 fused with mEmerald and miniAID-fused mCherry2

    Depositor

    Dario Palmieri

    Article

    Capece et al Cell Death Differ. 2023 Aug 3. doi: 10.1

    Insert

    OsTIR1-mEmerald-P2A-miniAID-mCherry2
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSUC2::GNSI (NrsR)

    Plasmid

    #121537

    Purpose

    This plasmid contains a CYC1pr driven GNSI reporter and flanking homology to SUC2. Digestion with NotI, SacI, and EcoRV, allows integration at the SUC2 locus.

    Depositor

    Alexey Merz

    Article

    Plemel et al Traffic. 2020 Nov 23. doi: 10.1111/tra.1

    Inserts

    CYC1 Promoter (CYC1 Budding Yeast)
    Nyv1 Cytosolic Domain (aa 6-230)
    Snc1 Transmembrane Domain (TMD)
    SUC2 CDS
    SUC2 terminator
    NrsR
    

    Use

    Tags

    mGFP5

    Expression

    Yeast

    Mutation

    M109I (Naturally occurring SNP) and The first 21 …

    Promoter

    Available since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-Lyn-sACt-mCherry

    Plasmid

    #138216

    Purpose

    Encodes residues 1-469 of rat soluble adenylyl cyclase (ADCY10) fused to mCherry; plasma membrane targeted.

    Depositor

    Jin Zhang

    Article

    Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.

    Insert

    Lyn-sACt-mCherry (Adcy10 Rat)
    

    Use

    Tags

    N-terminal targeting sequence from Lyn kinase and…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. EGFP Rab11a S25N pcDNA3.1 Zeo (+)

    Plasmid

    #206080

    Purpose

    dominant-negative Rab11a mutant - S25N mutation causes inhibition of Rab11a-dependent recycling

    Depositor

    Annette Dolphin

    Article

    Tran-Van-Minh et al J Neurosci. 2010 Sep 22;30(38):12856-67.

    Insert

    RAB11A (RAB11A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S25N

    Promoter

    CMV

    Available since

    Oct. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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