Addgene

We narrowed to 87,243 results for: lias

Showing: 1061 - 1080 of 87243 results

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pTK296

Plasmid

#59565

Purpose

SIN SP6 P1234 (nsP2 P726S) SGP(14) 2xK-turn Kozak EGFP-PEST

Depositor

Ron Weiss

Article

Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

Insert

SGP(14) 2xK-turn Kozak EGFP-PEST

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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ciCas9_pcDNA5

Plasmid

#100550

Purpose

Expresses ciCas9 in mammalian cells. Can be used to generate Flp-In and Flp-In T-REx stables.

Depositor

Dustin Maly

Article

Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

Insert

ciCas9

Use

CRISPR; Frt, flp-in

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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CLPIT Cry2PHR-mCh-LRP6c

Plasmid

#42961

Depositor

David Schaffer

Article

Bugaj et al Nat Methods. 2013 Feb 3. doi: 10.1038/nm

Insert

Cry2PHR-mCherry-LRP6c

Use

Retroviral

Tags

Expression

Mammalian

Mutation

LPR6 cytoplasmic domain only

Promoter

Tet-Off promoter

Available since

April 9, 2013

Availability

Academic Institutions and Nonprofits only

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pTK297

Plasmid

#59566

Purpose

SIN SP6 P1234 (nsP2 P726S) SGP(14) Kozak mKate-G8-L7Ae 4xFF4

Depositor

Ron Weiss

Article

Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

Insert

SGP(14) Kozak mKate-G8-L7Ae 4xFF4

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pIRESN2 GFP-uvr8at

Plasmid

#44969

Depositor

Thanos Halazonetis

Article

Crefcoeur et al Nat Commun. 2013;4:1779. doi: 10.1038/nc

Insert

GFP-UVR8 (UVR8 Mustard Weed, Synthetic)

Use

Tags

GFP

Expression

Mammalian

Mutation

deleted Methionine 1

Promoter

pCMV IE

Available since

June 6, 2013

Availability

Academic Institutions and Nonprofits only

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pTK299

Plasmid

#59568

Purpose

SIN SP6 P1234 (nsP2 P726S) SGP(14) Kozak L7Ae-P2A-EYFP 4xFF4

Depositor

Ron Weiss

Article

Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

Insert

SGP(14) Kozak L7Ae-P2A-EYFP 4xFF4

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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AAV-EFS-GFP

Plasmid

#170176

Purpose

Expresses GFP under the control of EF1a short promoter

Depositor

Xiaojun Lian

Article

Ream et al Stem Cell Reports. 2024 Mar 5:S2213-6711

Insert

GFP

Use

AAV

Tags

Expression

Mutation

Promoter

EFS promoter

Available since

July 14, 2024

Availability

Academic Institutions and Nonprofits only

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pTK105

Plasmid

#59562

Purpose

SIN SP6 P1234 (nsP2 P726S) SGP(14) 2xK-turn EYFP-PEST

Depositor

Ron Weiss

Article

Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

Insert

SGP(14) 2xK-turn EYFP-PEST

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pTK298

Plasmid

#59567

Purpose

SIN SP6 P1234 (nsP2 P726S) SGP(14) Kozak mKate-G8-L7Ae-PEST 4xFF4

Depositor

Ron Weiss

Article

Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

Insert

SGP(14) Kozak mKate-G8-L7Ae-PEST 4xFF4

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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GFP-GW182delta1

Plasmid

#11592

Depositor

Edward Chan

Article

Jakymiw et al Nat Cell Biol. 2005 Dec . 7(12):1267-74.

Insert

GW182 (TNRC6A Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

contains only aa1-498; SEE DEPOSITOR NOTES BELOW

Promoter

Available since

June 30, 2006

Availability

Academic Institutions and Nonprofits only
pCA27-pHR-MiniCMV-TriTag (mTagBFP)

Plasmid

#199458

Purpose

Expression of TriTag (mTagBFP) in mammalian cells

Depositor

Baohui Chen

Article

Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

Insert

TriTag

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

MiniCMV

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pTK101

Plasmid

#59561

Purpose

SIN SP6 P1234 (nsP2 P726S) SGP(14) mKate-G8-L7Ae-PEST 4xFF4

Depositor

Ron Weiss

Article

Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

Insert

SGP(14) mKate-G8-L7Ae-PEST 4xFF4

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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VV247: sdChR(C138S)-TS-GCaMP6f-ER in fck

Plasmid

#58522

Purpose

Expresses sdChR(C138S) fused to GCaMP6f in mammalian cells

Depositor

Adam Cohen

Article

Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

Insert

sdChR(C138S)-TS-GCaMP6f-ER

Use

Lentiviral

Tags

GCaMP6f

Expression

Mammalian

Mutation

changed Cysteine 138 to Serine

Promoter

a-CamKII

Available since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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p185 pK7-HDGF (GFP)

Plasmid

#11058

Depositor

Ramesh Shivdasani

Article

Tou et al Mol Cell Biol. 2004 Apr . 24(8):3132-9.

Insert

HDGF (Hdgf Rat)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2005

Availability

Academic Institutions and Nonprofits only
AAV EGFP donor pDY0404

Plasmid

#219862

Purpose

AAV EGFP donor

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Fell et al Nat Protoc. 2024 Dec 15. doi: 10.1038/s4

Insert

EGFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pCDFDuet-AtUBC8-S

Plasmid

#222713

Purpose

Expression AtUBC8-S in in bacterial cells

Depositor

Lei Wu

Article

Pu et al Biomolecules. 2024 Sep 25;14(10):1209. d

Insert

AtUBC8 (UBC8 Mustard Weed)

Use

Tags

S-Tag

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pGL 4.79-mCherry

Plasmid

#220137

Purpose

promoterless vector

Depositor

Monika Gulia-Nuss

Article

Pham et al iScience. 2024 Jul 8;27(8):110468. doi:

Type

Empty backbone

Use

Ixodes scapularis

Tags

Expression

Mutation

Promoter

No promoter

Available since

Jan. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-Cas12i2-T2A-EGFP

Plasmid

#188270

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Chen et al Innovation (Camb). 2022 May 26;3(4):1002

Insert

Cas12i2

Use

CRISPR

Tags

Expression

Mammalian

Mutation

none

Promoter

CAG

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-AaCas12b-T2A-EGFP

Plasmid

#188271

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Chen et al Innovation (Camb). 2022 May 26;3(4):1002

Insert

AaCas12b

Use

CRISPR

Tags

Expression

Mammalian

Mutation

none

Promoter

CAG

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pTSB-FICAT_Payload-Esp3I

Plasmid

#179380

Purpose

Transposon vector prepared for Esp3I golden gate cloning of Transgene

Depositor

Marc Güell

Article

Pallares-Masmitja et al Nat Commun. 2021 Dec 3;12(1):7071. doi:

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 1, 2022

Availability

Academic Institutions and Nonprofits only

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