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pSicoR-TMF1i1

Plasmid

#59624

Purpose

Expression of shRNA against human TMF1

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

TMF1 (TMF1 Human)

Use

RNAi

Expression

Mammalian

Promoter

U6

Available Since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only
p2CT-His-MBP-Lbu_C2c2_R1048A_H1053A

Plasmid

#83484

Purpose

Bacterial expression of L. buccalis C2c2 CRISPR effector (HEPN nuclease 2 inactive mutant).

Depositor

Jennifer Doudna

Article

East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

Insert

Lbu_C2c2_R1048A_H1053A

Use

CRISPR

Tags

His6-MBP

Expression

Bacterial

Mutation

R1048A, H1053A

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Barkor (FL)

Plasmid

#24394

Depositor

Qing Zhong

Article

Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

Insert

Barkor (ATG14 Human)

Tags

His and Myc

Expression

Mammalian

Available Since

April 1, 2010

Availability

Academic Institutions and Nonprofits only
pDGE331

Plasmid

#153240

Purpose

M1E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pEY12

Plasmid

#191018

Purpose

Fluorescent reporter driving panneuronal nuclear GCaMP6s expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

rab-3 (rab-3 Nematode)

Expression

Worm

Mutation

None

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pEY46

Plasmid

#191050

Purpose

glr-1 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

glr-1 (glr-1 Nematode)

Expression

Worm

Mutation

None

Available Since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pEY38

Plasmid

#191042

Purpose

unc-8 fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

unc-8 (unc-8 Nematode)

Expression

Worm

Mutation

None

Available Since

Nov. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pEY13

Plasmid

#191019

Purpose

Fluorescent reporter driving amphid and phasmid nuclear GCaMP6s expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

arrd-4 (ZK938.2 Nematode)

Expression

Worm

Mutation

None

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pEY20

Plasmid

#191026

Purpose

gcy-18 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

gcy-18 (gcy-18 Nematode)

Expression

Worm

Mutation

None

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pRK5-mFzd2-1D4

Plasmid

#42264

Depositor

Chris Garcia, Jeremy Nathans

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Frizzled 2 (Fzd2 Mouse)

Tags

1D4

Expression

Mammalian

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
pRK5-mFzd4-1D4

Plasmid

#42266

Depositor

Chris Garcia, Jeremy Nathans

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Frizzled 4 (Fzd4 Mouse)

Tags

1D4

Expression

Mammalian

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
pTYF-super-mfvAChT-1.8k-Venus-WPRE

Plasmid

#156389

Purpose

Expresses Venus under vAChT gene promoter

Depositor

Shuji Kaneko

Article

Nagai et al Biochem Biophys Res Commun. 2019 Oct 22;

Inserts

Venus
Gal4p65

Use

Lentiviral

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-FAPP1

Plasmid

#109503

Purpose

Muscle specific zebrafish expression of mKate2 fused to the PH domain from Human pleckstrin homology domain containing A3 (PLEKHA3). Binds PtdIns4P

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

actc1b-mKate2-FAPP1

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-2xFYVEhgs

Plasmid

#109505

Purpose

Muscle specific zebrafish expression of mKate2 fused to mouse 2x PH domain from HGF regulated tyrosine kinase substrate (Hgs). Binds PtdIns3P

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

actc1b-mKate2-2xFYVEhgs

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp15-DARPinOFF7

Plasmid

#182399

Purpose

Bacterial expression of Vesicle Nucleating peptide15-DARPinOFF7 fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp15-DARPinOFF7

Tags

Vesicle Nucleating peptide 15 (VNp15)

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1a-tetR-T2A-iRFP

Plasmid

#176028

Purpose

construct expresses tet repressor (tetR) to reduce leakiness together with the fluorochrome iRFP720

Depositor

Irmela Jeremias

Article

Liu et al Biomark Res. 2020 Sep 16;8:46. doi: 10.1

Inserts

tetR
iRFP

Use

Lentiviral

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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TF-bio-His

Plasmid

#52184

Purpose

Expresses full-length Serotransferrin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

TF (TF Human)

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pFUSE-DEST_R1R4

Plasmid

#97186

Purpose

For fusion gene cloning. Recombine Gene A + Gene B into this Gateway Destination Vector

Depositor

Kenneth Scott

Article

Lu et al Cancer Res. 2017 May 16. pii: canres.274

Type

Empty backbone

Use

Lentiviral; Gateway

Tags

V5

Expression

Mammalian

Promoter

EF1 alpha

Available Since

Aug. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pACE-GIRK4-S

Plasmid

#172425

Purpose

Expression of full-length, human GIRK4 with C-terminal StrepTag II

Depositor

Arthur Laganowsky

Article

Qiao et al Biochemistry. 2021 Dec 21;60(50):3813-38

Insert

GIRK4 (KCNJ5 Human)

Tags

Streptag II

Expression

Insect

Available Since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pLHCX-Flag-mPKM2(K433E)

Plasmid

#42514

Depositor

Dimitrios Anastasiou

Article

Anastasiou et al Science. 2011 Dec 2;334(6060):1278-83. d

Insert

Pyruvate Kinase M2 Lys433->Glu

Use

Retroviral

Tags

Flag

Mutation

Changed lysine 433 to glutamate

Available Since

March 19, 2013

Availability

Academic Institutions and Nonprofits only