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pLentiCMV Puro DEST ERKKTRClover

Plasmid

#59150

Purpose

Lentiviral vector to express ERK KTR mClover under CMV promoter (With Puromycin Resistance)

Depositor

Markus Covert

Article

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

ERK Kinase Translocation Reporter

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 19, 2014

Availability

Academic Institutions and Nonprofits only

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MAC_N_STA5B

Plasmid

#167800

Purpose

Expresses STA5B in mammalian cells

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

STA5B (STAT5B Human)

Tags

MAC

Expression

Mammalian

Mutation

no mutations

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pGWB-cLUC

Plasmid

#174051

Purpose

Gateway compatible plasmid containing C-terminal luciferase for split-luciferase complementation assay

Depositor

Alberto Macho

Article

Yu et al PLoS Pathog. 2020 Sep 25;16(9):e1008933.

Type

Empty backbone

Use

Luciferase

Expression

Plant

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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p413TEF1-ATR1(pYL573)

Plasmid

#178288

Purpose

Expresses NADPH-cytochrome P450 reductase 1 from A. thaliana (ATR1) in Saccharomyces cerevisiae. Centromeric HIS, PTEF1-ATR1-TCYC1

Depositor

Yanran Li

Article

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Insert

ATR1

Expression

Yeast

Promoter

TEF1

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pPB-EF1a-MegaGate-HA-Blast

Plasmid

#175270

Purpose

MegaGate destination with C-terminal HA tag and blasticidin resistance

Depositor

George Church

Article

Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

Type

Empty backbone

Tags

HA

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST mpeg1:tomato-CAAX

Plasmid

#135215

Purpose

Red tagged macrophages

Depositor

David Tobin

Article

Oehlers et al Nature. 2015 Jan 29;517(7536):612-5. doi

Insert

mpeg1 and tdTomato

Use

Zebrafish expression

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti6-H2B-mCherry

Plasmid

#89766

Purpose

Lentivirus destination vector for stable expression of mCherry-tagged histone H2B

Depositor

Torsten Wittmann

Article

Pemble et al J Cell Sci. 2017 Apr 15;130(8):1404-1412

Insert

Histone H2B (H2BC11 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pLX-EF1a_NKX2-1_CdTag-HA

Plasmid

#221493

Purpose

NKX2-1 short isoform lentiviral overexpression of C-terminal dTag fusion

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

FKBP F36V

Expression

Mammalian

Promoter

EF1a

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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CAG FUCCI

Plasmid

#175266

Purpose

Bicistronic FUCCI construct driven by a CAG promoter. Contains I-SceI sites for transgenesis

Depositor

James Monaghan

Article

Duerr et al Development. 2022 Mar 15;149(6). pii: 27

Insert

FUCCI

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-HDAC2

Plasmid

#222553

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of HDAC2

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

HDAC2 (HDAC2 Human)

Expression

Mammalian

Available Since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-TET1

Plasmid

#222572

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of TET1

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

TET1 (TET1 Human)

Expression

Mammalian

Available Since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pFRT_TO_FlagHA_TIA1

Plasmid

#106093

Purpose

gene expression of FLAGHA-TIA1

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

TIA1 (TIA1 Human)

Tags

FlagHA

Expression

Mammalian

Available Since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only

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POLN_pDEST17

Plasmid

#64885

Purpose

bacterial expression of POLN for purification

Depositor

Richard Wood

Article

Takata et al Nucleic Acids Res. 2010 Jun;38(10):3233-

Insert

POLN (POLN Human)

Tags

Flag and His

Expression

Bacterial

Promoter

T7

Available Since

May 21, 2015

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-BOLL

Plasmid

#222526

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of BOLL

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

BOLL (BOLL Human)

Expression

Mammalian

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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GAL4BD-VP16/pDON207

Plasmid

#201230

Purpose

GAL4-VP16 transcription factor

Depositor

Michael Ayliffe

Article

Chen et al Plant J. 2023 Jun;114(6):1209-1226. doi:

Insert

GAL4BD-VP16

Expression

Plant

Available Since

July 21, 2023

Availability

Academic Institutions and Nonprofits only

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pMA122 - peel-1 negative selection

Plasmid

#34873

Purpose

Negative selection marker for use with the Mos-1 mediated system of creating targeted transgene insertions and targeted deletions in C. elegans

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

Insert

peel-1 (peel-1 Nematode)

Use

Worm targeting

Available Since

Feb. 1, 2012

Availability

Academic Institutions and Nonprofits only

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plenti-CAG -IRES-GFP

Plasmid

#69047

Purpose

Lentiviral backbone for GFP coexpression

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Type

Empty backbone

Use

Lentiviral

Tags

IRES-GFP

Expression

Mammalian

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pInducer20-Blast

Plasmid

#109334

Purpose

pInducer20 with added blasticidin resistance

Depositor

Jean Cook

Article

Matson et al Elife. 2017 Nov 17;6. doi: 10.7554/eLife

Type

Empty backbone

Use

Lentiviral; Inducible expression

Expression

Mammalian

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pME phluorin2-gpi

Plasmid

#73793

Purpose

ratiometric pHluorin2 with a gpi linkage sequence in a middle entry clone

Depositor

David Raible

Article

Stawicki et al Dis Model Mech. 2014 Jul;7(7):847-56. do

Insert

ratiometric pHluorin2-gpi

Use

Middle entry clone

Available Since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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pER8 cHA-FG1

Plasmid

#118689

Purpose

Plant inducible expression

Depositor

Kristiina Himanen

Article

Pavicic et al Front Plant Sci. 2017 Mar 28;8:416. doi:

Insert

AT3G07200 (AT3G07200 Mustard Weed)

Tags

HA

Expression

Plant

Promoter

LexA

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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