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pPEntry

Plasmid

#207673

Purpose

Entry vector for Trio vector system, with DAPG-inducible promoter

Depositor

Ramon Gonzalez

Article

Lee et al Metab Eng. 2024 Mar;82:262-273. doi: 10.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pCEntry

Plasmid

#207672

Purpose

Entry vector for Trio vector system, with cumate-inducible promoter

Depositor

Ramon Gonzalez

Article

Lee et al Metab Eng. 2024 Mar;82:262-273. doi: 10.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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FB407

Plasmid

#203628

Purpose

Promoter of the elongation factor 1-alpha gene from P. digitatum (PDIG_59570).

Depositor

Jose Marcos

Article

Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1

Insert

Pef1A

Use

Synthetic Biology

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCRISPomyces-AsCas12j-2

Plasmid

#191655

Purpose

All-in-one editing CRISPR-Cas construct for one-step genome editing of Streptomyces using AsCas12j-2

Depositor

Fong Tian Wong

Article

Tan et al Biomolecules. 2024 Apr 16;14(4):486. doi

Insert

AsCas12j-2

Expression

Bacterial

Promoter

rpsL(XC)-BbsI

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pET22b-T5-mad

Plasmid

#188998

Purpose

Express wildtype madanin-1

Depositor

Han Xiao

Article

Chen et al Nat Commun. 2022 Sep 16;13(1):5434. doi:

Insert

wildtype madanin-1

Expression

Bacterial

Available Since

Sept. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pRK010

Plasmid

#69874

Purpose

expression of Channelrhodopsin-2 XXL (extra high expression and long open state, D156C) mutant

Depositor

Robert Kittel, Georg Nagel

Article

Dawydow et al Proc Natl Acad Sci U S A. 2014 Sep 23;11

Insert

Channelrhodopsin-2

Use

Unspecified

Mutation

D156C (XXL)

Promoter

Drosophila Hsp70

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_VPR_Puro

Plasmid

#167895

Purpose

PiggyBac compatible plasmid expressing Cas9-VPR

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

Cas9-VPR

Use

CRISPR

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_VPR_EYFP

Plasmid

#167890

Purpose

PiggyBac compatible plasmid expressing Cas9-VPR

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

Cas9-VPR

Use

CRISPR

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_VPR_EBFP2

Plasmid

#167889

Purpose

PiggyBac compatible plasmid expressing Cas9-VPR

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

Cas9-VPR

Use

CRISPR

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pX018

Plasmid

#167144

Purpose

MoClo-compatible Level 0-SP promoterless vector encoding Neonothopanus nambi luciferase nnLuz codon-optimised for expression in Nicotiana benthamiana and Pichia pastoris

Depositor

Karen Sarkisyan

Article

Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

Insert

fungal luciferase, nnLuz

Use

Luciferase and Synthetic Biology

Available Since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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pTalpha1-EGFP-N1

Plasmid

#133929

Purpose

Neuron-specific expression of EGFP

Depositor

Frank Bradke, Sebastián Dupraz

Article

Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.

Insert

EGFP

Expression

Mammalian

Mutation

EGFP

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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MTK0_037

Plasmid

#123974

Purpose

Encodes the hROSA26 BxBI landing pad as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

hROSA26 BxBI LP

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK3_001

Plasmid

#123952

Purpose

Encodes nuclear localized PHR domain of CRY2 fused to activating domain of p65 as Type 3 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

3XNLS::CRY2PHR::p65

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK0_018

Plasmid

#123933

Purpose

Encodes the spacer TU with connectors LS R1 as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

TULSR1 - spacer

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK0_019

Plasmid

#123934

Purpose

Encodes the spacer TU with connectors L1 R2 as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

TUL1R2 spacer

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK0_020

Plasmid

#123935

Purpose

Encodes the spacer TU with connectors L2 R3 as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

TUL2R3 spacer

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK0_022

Plasmid

#123937

Purpose

Encodes the spacer TU with connectors L4 R5 as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

TUL4R5 spacer

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK0_023

Plasmid

#123938

Purpose

Encodes the spacer TU with connectors L5 R6 as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

TUL5R6 spacer

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK0_024

Plasmid

#123939

Purpose

Encodes the spacer TU with connectors L6 R7 as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

TUL6R7 spacer

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK0_025

Plasmid

#123940

Purpose

Encodes the spacer TU with connectors L7 R8 as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

TUL7R8 spacer

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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