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pMEG_BB2_YL001

Plasmid

#117824

Purpose

Transcription unit of Y. lipolytica for GoldenMOCS

Depositor

Michael Sauer

Article

Egermeier et al FEMS Microbiol Lett. 2019 Jan 29. pii: 5

Insert

YlpTEF_YlGUT1_YlCyc1TT

Expression

Bacterial

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pTK308

Plasmid

#114689

Purpose

sgRNA targeting DHC's C-terminal locus

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

DHC-C sgRNA

Available Since

Sept. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.Syn-FLEX.iGABASnFR.F102Y.Y137L.mRuby3

Plasmid

#112171

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Insert

iGABASnFR.F102Y.Y137L

Use

AAV

Tags

mRuby3

Mutation

F102Y.Y137L

Promoter

Synapsin

Available Since

July 16, 2018

Availability

Academic Institutions and Nonprofits only

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pTSara*SZ

Plasmid

#107957

Purpose

Arabinose-inducible split T7*RNAP with SynZIP domains 17/18

Depositor

Jim Haseloff

Article

Boehm et al Nat Commun. 2018 Feb 22;9(1):776. doi: 1

Insert

T7RNAP (modified)

Use

Synthetic Biology

Expression

Bacterial

Mutation

R632S

Available Since

April 2, 2018

Availability

Academic Institutions and Nonprofits only

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pexA2_2hb_ruleB

Plasmid

#89670

Purpose

Plasmid for the construction of DNA-protein hybrid nanostructures. Template for the two-helix-bundle shown in figure 2E,F,G of associated article.

Depositor

Hendrik Dietz

Article

Praetorius et al Science. 2017 Mar 24;355(6331). pii: eaa

Insert

2hb_ruleB

Use

Unspecified

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pexA2_ring

Plasmid

#89672

Purpose

Plasmid for the construction of DNA-protein hybrid nanostructures. Template for the ring shown in figure 3C of associated article.

Depositor

Hendrik Dietz

Article

Praetorius et al Science. 2017 Mar 24;355(6331). pii: eaa

Insert

ring

Use

Unspecified

Available Since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

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Bxbi GFP MCP Reporter

Plasmid

#78225

Purpose

synthetic gene circuit

Depositor

Timothy Lu

Article

Rubens et al Nat Commun. 2016 Jun 3;7:11658. doi: 10.

Insert

synthetic gene circuit

Available Since

May 11, 2017

Availability

Academic Institutions and Nonprofits only

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p3E-HA no-pA

Plasmid

#82593

Purpose

3' entry vector containing HA epitope fused to C-terminus of mCherry without pA for epitope labeling/purification

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

HA tag (no pA)

Promoter

None

Available Since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pNP-P+

Plasmid

#81229

Purpose

the CMV promoter of pCS2 has been replaced by a 931-bp fragment of the mitfa promoter and 5'UTR

Depositor

James Lister, David Raible

Article

Lister et al Dev Biol. 2001 Sep 15;237(2):333-44.

Type

Empty backbone

Use

Xenopus//avian/zebrafish

Expression

Mammalian

Promoter

mitfa

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pGCaMP5

Plasmid

#78705

Purpose

GCaMP5 under the Toxoplasma SAG1 5' UTR. No Toxoplasma selectable marker

Depositor

Sebastian Lourido

Article

Sidik et al J Biol Chem. 2016 Apr 29;291(18):9566-80

Insert

GCaMP5

Promoter

SAG1 5' UTR

Available Since

May 27, 2016

Availability

Academic Institutions and Nonprofits only

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pAIP4

Plasmid

#233686

Purpose

Cloning vector with an N-terminal fusion of 10x His-tagged SUMO-1, followed by a Golden Gate assembly site for seamless insertion.

Depositor

Christopher Bahl

Insert

Smt3 (SMT3 )

Tags

HisTag

Promoter

T7

Available Since

April 7, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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NP-11-63

Plasmid

#234358

Purpose

Expressing and purifying R.rubrum rubisco using a his-sumo tag

Depositor

David Savage

Article

Prywes et al Nature. 2025 Jan 22. doi: 10.1038/s41586

Insert

R.rubrum rubisco

Use

Synthetic Biology

Tags

N-term SUMO tag

Promoter

T7

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pSrRGECO1

Plasmid

#222940

Purpose

Expresses RGECO1 (fluorescent calcium indicator) and pac (puromycin resistance) in the Choanoflagellate, Salpingoeca rosetta

Depositor

Pawel Burkhardt

Article

Colgren et al Sci Adv. 2025 Jan 10;11(2):eadr7434. doi

Insert

RGECO1

Use

Salpingoeca rosetta expression

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pVEntry

Plasmid

#207674

Purpose

Entry vector for Trio vector system, with vanillate-inducible promoter

Depositor

Ramon Gonzalez

Article

Lee et al Metab Eng. 2024 Mar;82:262-273. doi: 10.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pPEntry

Plasmid

#207673

Purpose

Entry vector for Trio vector system, with DAPG-inducible promoter

Depositor

Ramon Gonzalez

Article

Lee et al Metab Eng. 2024 Mar;82:262-273. doi: 10.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pCEntry

Plasmid

#207672

Purpose

Entry vector for Trio vector system, with cumate-inducible promoter

Depositor

Ramon Gonzalez

Article

Lee et al Metab Eng. 2024 Mar;82:262-273. doi: 10.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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FB407

Plasmid

#203628

Purpose

Promoter of the elongation factor 1-alpha gene from P. digitatum (PDIG_59570).

Depositor

Jose Marcos

Article

Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1

Insert

Pef1A

Use

Synthetic Biology

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCRISPomyces-AsCas12j-2

Plasmid

#191655

Purpose

All-in-one editing CRISPR-Cas construct for one-step genome editing of Streptomyces using AsCas12j-2

Depositor

Fong Tian Wong

Article

Tan et al Biomolecules. 2024 Apr 16;14(4):486. doi

Insert

AsCas12j-2

Expression

Bacterial

Promoter

rpsL(XC)-BbsI

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pET22b-T5-mad

Plasmid

#188998

Purpose

Express wildtype madanin-1

Depositor

Han Xiao

Article

Chen et al Nat Commun. 2022 Sep 16;13(1):5434. doi:

Insert

wildtype madanin-1

Expression

Bacterial

Available Since

Sept. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pRK010

Plasmid

#69874

Purpose

expression of Channelrhodopsin-2 XXL (extra high expression and long open state, D156C) mutant

Depositor

Robert Kittel, Georg Nagel

Article

Dawydow et al Proc Natl Acad Sci U S A. 2014 Sep 23;11

Insert

Channelrhodopsin-2

Use

Unspecified

Mutation

D156C (XXL)

Promoter

Drosophila Hsp70

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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