We narrowed to 59,461 results for: %s

1. pGEM-HE-bPAC(R278A)-Myc-eYFP

   Plasmid

   #165488

   Purpose

   Xenopus oocyte expression of soluble photoactivatable adenylyl cyclase derived from bPAC with reduced dark activity

   Depositor

   Thomas Oertner

   Article

   Yang et al BMC Biol. 2021 Oct 18;19(1):227. doi: 10

   Insert

   bPAC(R278A)-Myc-eYFP
   

   Use

   Cdna expression, xenopus oocyte

   Tags

   EYFP and Myc

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-Bmpr2 gRNA resistant

   Plasmid

   #163408

   Purpose

   Expresses BMPR-2 resistant to the gRNAs(#163388-90) in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   Bmpr2 (Bmpr2 Mouse)
   

   Expression

   Mammalian

   Mutation

   gRNA-targeting sequences are substituted with nuc…

   Available Since

   Aug. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGB_3alpha2 OplacI:mini35S:RDF:Tnos (GB1534)

   Plasmid

   #160617

   Purpose

   TU for the regulated expression of the PhiC31 phage recombination directionality factor (RDF) gene driven by a synthetic promoter consisting of the LacI operator fused to the minimal 35S promoter.

   Depositor

   Diego Orzaez

   Article

   Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

   Insert

   RDF
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   mini35s

   Available Since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUPD2 PhiC31 RL (attR:P35S:TMtb:attL) (GB1506)

   Plasmid

   #160582

   Purpose

   35S promoter inverted sequence and the Mtb terminator flanked by two opposing PhiC31 site-specific recombination sites (attR and attL)

   Depositor

   Diego Orzaez

   Article

   Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

   Insert

   attR:P35S:TMtb:attL
   

   Use

   Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGB3 alpha2 P35S:RDF:T35S (GB1508)

   Plasmid

   #160613

   Purpose

   TU for the constitutive expression of Streptomyces phage PhiC31-encoded recombination directionality factor (RDF) (PhiC31 gp3)

   Depositor

   Diego Orzaez

   Article

   Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

   Insert

   RDF
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   35S

   Available Since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGB3 alpha2 OplexA:mini35S:PhiC31int:Tnos (GB1529)

   Plasmid

   #160614

   Purpose

   TU for the regulated expression of the PhiC31 phage integrase gene driven by a synthetic promoter consisting of the LexA operator fused to the minimal 35S promoter.

   Depositor

   Diego Orzaez

   Article

   Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

   Insert

   PhiC31
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   mini35s

   Available Since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGB_3alpha1 OplacI:mini35S:phiC31:Tnos (GB1530)

   Plasmid

   #160615

   Purpose

   TU for the regulated expression of the PhiC31 phage integrase gene driven by a synthetic promoter consisting of the LacI operator fused to the minimal 35S promoter.

   Depositor

   Diego Orzaez

   Article

   Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

   Insert

   PhiC31
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   mini35s

   Available Since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-EGxxFP-PIGA

   Plasmid

   #153958

   Purpose

   Used for EGxxFP assay with PIGA intron 5 and exon 6 sequences

   Depositor

   Hiroyuki Konishi

   Article

   Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

   Insert

   PIGA (a 401-bp fragment from intron 5 and exon 6)
   

   Use

   A plasmid specific for egxxfp assay

   Tags

   N/A

   Expression

   Mammalian

   Mutation

   No mutation

   Promoter

   CAG promoter

   Available Since

   Dec. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. Donor-PIGAmut

   Plasmid

   #153548

   Purpose

   Used as a donor vector for the targeted knock-in of a mutation in PIGA exon 6

   Depositor

   Hiroyuki Konishi

   Article

   Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

   Insert

   mutant PIGA (a 1,991-bp fragment from intron 5 and exon 6 with a 3-bp mutation in exon 6)
   

   Use

   Donor plasmid

   Tags

   N/A

   Mutation

   a 3-bp mutation in exon 6

   Promoter

   No promoter

   Available Since

   Dec. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-hSyn-DIO-HA-CB2SH3(-)-IRES-mCitrine

    Plasmid

    #160069

    Purpose

    Conditionally overexpress collybistin (CB2SH3-) and mCitrine

    Depositor

    Angel de Blas

    Article

    George et al eNeuro. 2021 Jun 2. pii: ENEURO.0561-20.

    Insert

    ARHGEF9 (Arhgef9 Rat)
    

    Use

    AAV

    Tags

    HA- in CB2SH3

    Promoter

    human Synapsin promoter

    Available Since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET_4E1H.95

    Plasmid

    #155213

    Purpose

    Expresses 4E1H.95 de novo protein in E.coli

    Depositor

    Bruno Correia

    Article

    Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

    Insert

    4E1H.95
    

    Tags

    6x His Tag

    Expression

    Bacterial

    Available Since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET_4H.01

    Plasmid

    #155210

    Purpose

    Expresses 4H.01 de novo designed protein in E.coli

    Depositor

    Bruno Correia

    Article

    Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

    Insert

    4H.01
    

    Tags

    6x His Tag

    Expression

    Bacterial

    Available Since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. 4E1H.95_LUMABS

    Plasmid

    #155208

    Purpose

    Expresses 4E1H.95_LUMABS sensor in E.coli

    Depositor

    Bruno Correia

    Article

    Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

    Insert

    4E1H.95_LUMABS
    

    Tags

    6x His Tag

    Available Since

    Aug. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET_4E2H.210

    Plasmid

    #155212

    Purpose

    Expresses 4E2H.210 de novo protein in E.coli

    Depositor

    Bruno Correia

    Article

    Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

    Insert

    4E2H.210
    

    Tags

    6x His Tag

    Expression

    Bacterial

    Available Since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET_3E2H.37

    Plasmid

    #155211

    Purpose

    Expresses 3E2H.37 de novo designed protein in E.coli

    Depositor

    Bruno Correia

    Article

    Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

    Insert

    3E2H.37
    

    Tags

    6x His Tag

    Expression

    Bacterial

    Available Since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-T7-AcrIIA1(T16A)-NLS(SV40) (KAC511)

    Plasmid

    #133797

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized AcrIIA1(T16A) with C-terminal NLS (SV40)

    Depositor

    Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Osuna et al Cell Host Microbe. 2020 Apr 18. pii: S19

    Insert

    human codon optimized AcrIIA1(T16A)
    

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Mutation

    T16A

    Promoter

    CMV and T7

    Available Since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-T7-AcrIIA1-Leu-NLS(SV40) (KAC440)

    Plasmid

    #133799

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized Leu-AcrIIA1 with C-terminal NLS (SV40)

    Depositor

    Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Osuna et al Cell Host Microbe. 2020 Apr 18. pii: S19

    Insert

    human codon optimized Leu-AcrIIA1
    

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    CMV and T7

    Available Since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-T7-AcrIIA1-Eriv-NLS(SV40) (KAC442)

    Plasmid

    #133800

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized Eriv-AcrIIA1 with C-terminal NLS (SV40)

    Depositor

    Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Osuna et al Cell Host Microbe. 2020 Apr 18. pii: S19

    Insert

    human codon optimized Eriv-AcrIIA1
    

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    CMV and T7

    Available Since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. tol2-lyzC-miR-23a-2-dendra2

    Plasmid

    #105435

    Purpose

    overexpression of microRNA and Dendra2 in Zebrafish

    Depositor

    Qing Deng

    Article

    Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

    Insert

    miR-23a-2 Dendra2 (mir23a-2 Zebrafish)
    

    Use

    Zebrafish

    Tags

    N/A

    Available Since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. hsa-cdk2 3'UTR sicheck2

    Plasmid

    #121125

    Purpose

    luciferase assay

    Depositor

    Qing Deng

    Article

    Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

    Insert

    hsa-cdk2 3'UTR (CDK2 Human)
    

    Expression

    Bacterial

    Available Since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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