We narrowed to 17,098 results for: IGH@

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VfAU1-LOV_P2A_casp9

Plasmid

#131224

Purpose

Expresses Ha-tagged VfAU1-LOV and myc-tagged casp9 (domains of Opto-casp9) separated by P2A site

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

VfAU1-LOV-HA_P2A_myc-casp9

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-haA3A-CBE-A

Plasmid

#220892

Purpose

Express haA3A-CBE-A (with SpCas9) in mammalian cells

Depositor

Dali Li

Article

Yang et al Nat Chem Biol. 2024 Mar 29. doi: 10.1038

Insert

haA3A-CBE-A

Expression

Mammalian

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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pTol2-elavl3-WHaloCaMP1a-EGFP

Plasmid

#205311

Purpose

Expression of WHaloCaMP1a-EGFP in zebrafish from the elavl3 promoter (pan-neuronal expression)

Depositor

Eric Schreiter

Article

Farrants et al bioRxiv. 2023 Jul 19:2023.07.18.549527.

Insert

WHaloCaMP1a-EGFP

Use

Zebrafish expression

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-haA3A-CBE-G-NG

Plasmid

#220897

Purpose

Express haA3A-CBE-G-NG (with SpCas9-NG variant) in mammalian cells

Depositor

Dali Li

Article

Yang et al Nat Chem Biol. 2024 Mar 29. doi: 10.1038

Insert

haA3A-CBE-G-NG

Expression

Mammalian

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-haA3A-CBE-G

Plasmid

#220894

Purpose

Express haA3A-CBE-G (with SpCas9) in mammalian cells

Depositor

Dali Li

Article

Yang et al Nat Chem Biol. 2024 Mar 29. doi: 10.1038

Insert

haA3A-CBE-G

Expression

Mammalian

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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pEN_TT 3xFLAG-CSE1L

Plasmid

#192295

Purpose

Gateway entry vector for an inducible 3xFLAG-CSE1L

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

CSE1L (CSE1L Human)

Use

Gateway entry vector

Tags

3xFLAG

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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Human HUWE1 (3993-4374)

Plasmid

#110754

Purpose

Bacterial Expression of HUWE1 HECT domain

Depositor

David Komander

Article

Michel et al Mol Cell. 2017 Oct 5;68(1):233-246.e5. d

Insert

HUWE1 (HUWE1 Human)

Tags

N-His6-3C

Expression

Bacterial

Promoter

T7

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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mTagRFP-T-Clathrin-15

Plasmid

#58005

Purpose

Localization: Clathrin Vesicles, Excitation: 555, Emission: 584

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

Clathrin (CLTB Human)

Tags

mTagRFP

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pMCB619

Plasmid

#171011

Purpose

CRISPR sgRNA vector (puro-BFP with BstXI/BlpI digest sites).

Depositor

Alice Ting

Article

Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

Insert

U6:sgRNA_Puro-T2A-BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mU6

Available Since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pProEx Rab5C(S30C) CL

Plasmid

#224273

Purpose

Protein purification of Rab5C (S30C)

Depositor

Kevan Shokat

Article

Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

Insert

Rab5C (RAB5C Human)

Expression

Bacterial

Mutation

S30C

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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eGFPbait-E2A-KalTA4-pA donor vector

Plasmid

#61069

Purpose

for CRISPR/Cas9 mediated insertion of E2A-KalTA4; to be used in combination with a eGFP specific sgRNA (e.g. Plasmid 61051)

Depositor

Filippo Del Bene

Article

Auer et al Genome Res. 2014 Jan;24(1):142-53. doi:

Inserts

eGFPbait
KalTA4

Use

Zebrafish expression

Tags

E2A

Available Since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

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pLV_PGK_LTB4R

Plasmid

#208087

Purpose

Mammalian expression of the wild-type human LTB4 receptor LTB4R.

Depositor

Sean R Collins

Article

Lundgren et al Sci Signal. 2023 Oct 3;16(805):eadd1845.

Insert

LTB4R (LTB4R Human)

Use

Lentiviral

Expression

Mammalian

Mutation

none

Promoter

PGK

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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mRuby2-Tubulin-C-18

Plasmid

#55915

Purpose

Localization: Microtubules, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

Tubulin (TUBA1B Human)

Tags

mRuby2

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pAAV-SEO-CaMKII-TVA-P2A-N2cG

Plasmid

#172363

Purpose

Targeting envA-pseodotyped RVdG-CVS-N2c vectors for specific retrograde labeling from excitatory cortical neurons which do not express cre-recombinase

Depositor

Yoav Ben-Simon, Peter Jonas

Article

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

TVA + B19-N2cG

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

CaMKIIa

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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B2342_BTB-mCherry

Plasmid

#215095

Purpose

Expresses BCL6 BTB(1-129)-mCherry in mammalian cells

Depositor

Chandra Tucker

Article

Hernandez-Candia et al Nat Chem Biol. 2024 Jan 8. doi: 10.1038/

Insert

BCL6 BTB (BCL6 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyso-InPAkt

Plasmid

#184053

Purpose

Lysosome-targeted FRET-based biosensor for monitoring 3-phosphoinositide dynamics in living cells.

Depositor

Jin Zhang

Article

Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

Insert

Lyso-InPAkt

Tags

Lysosome-associated membrane protein 1 (LAMP1).

Expression

Mammalian

Promoter

CMV

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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mCerulean3-ER-5

Plasmid

#55418

Purpose

Localization: Endoplasmic Reticulum, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

ER (CALR Human)

Tags

mCerulean3

Expression

Mammalian

Mutation

ER Signal Peptide

Promoter

CMV

Available Since

Dec. 4, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA-D2s-L-Venus

Plasmid

#19966

Depositor

Jonathan Javitch

Article

Guo et al EMBO J. 2008 Sep 3. 27(17):2293-304.

Insert

D2 short variant-monomeric Venus (DRD2 Human, Aequorea Victoria)

Tags

Flag and monomeric Venus

Expression

Mammalian

Available Since

Jan. 27, 2009

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_mNeongreen-Etanercept

Plasmid

#182420

Purpose

Bacterial expression of mNeongreen-Etanercept fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

mNeongreen-Etanercept (TNFRSF1B Human)

Tags

mNeongreen

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pAWPSG-Ptet-nCas9-BE

Plasmid

#195739

Purpose

Express APOVEC1-nCas9(D10A)-UGI using aTC inducible system in Methanotroph or E. coli

Depositor

Seung-Goo Lee

Article

Jeong et al Chem Eng J, 2023

Insert

Tet repressor protein / APOBEC1-nCas9(D10A)-UGI

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

Ptet(Tetracycline inducible protein)

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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