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We narrowed to 57,324 results for: plasmid

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Showing: 10861 - 10880 of 57324 results

  1. pQE81L-KOFP7

    Plasmid
    #160330
    Purpose
    This plasmid encodes an engineered oxygen-independent flavin binding fluorescent protein with N-terminal 6xHis tag. Its quantum yield is comparable to EGFP.
    Depositor
    Sang Taek Jung
    Publication
    Ko et al J Agric Food Chem. 2020 May 27;68(21):58
    Insert
    KOFP7
    Tags
    6xHis tag
    Expression
    Bacterial
    Promoter
    T5
    Available since
    March 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    160330-plasmid-map-sequence-id-326602

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  5. pSpdCas9-hudTET1CD-T2A-GFP (PX458)

    Plasmid
    #129026
    Purpose
    inactivated control (targeted DNA demethylation),expression of dCas9-hudTET1CD-T2A-EGFP and cloning backbone for sgRNA
    Depositor
    Julia K Polansky
    Publication
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Insert
    dCas9-hudTET1CD, SgRNA cloning site
    Tags
    HA-Tag, NLS and T2A-EGFP
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A), catalytic domain huTET1 inact…
    Promoter
    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    129026-plasmid-map-sequence-id-256128

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  12. pJ204-loxP-EF1a-mCherry-NeoR-lox2272-BGHpA-siteA-HDR

    Plasmid
    #154826
    Purpose
    Landing pad for Cre/lox-based recombinase-mediated cassette exchange (RMCE) in CHO cells, donor plasmid for CRISPR/Cas9-mediated targeted integration in CHO cells (site A)
    Depositor
    Lise Marie Grav
    Publication
    Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102
    Inserts
    mCherry
    NeoR
    ZsGreen1-DR
    Use
    CRISPR and Cre/Lox
    Tags
    PEST
    Expression
    Mammalian
    Promoter
    CMV, EF1a, and SV40 early promoter
    Available since
    Aug. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    154826-plasmid-map-sequence-id-306204

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  13. plenti-As-Cas12a-2xNLS

    Plasmid
    #155047
    Purpose
    Lentiviral vector expressing human codon-optimized _C-terminal Myc-tagged _Acidaminococcus sp. BV3L6 (As)-Cas12a nuclease with N- and C-terminal NLS and Neomycin/G418/Geneticin resistance from EF-1alpha promoter
    Depositor
    Benjamin Blencowe, Jason Moffat
    Publication
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    AsCas12a
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS, Myc-tag and SV40 NLS
    Expression
    Mammalian
    Promoter
    EF-1a
    Available since
    Aug. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    155047-plasmid-map-sequence-id-308054

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  15. pcDNA3-sPDGFRalpha-Fc

    Plasmid
    #136438
    Purpose
    Mammalian expression plasmid for soluble ectodomain of PDGFR-alpha fused to IgG1 Fc
    Depositor
    Erik Procko
    Publication
    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.
    Insert
    PDGFRalpha (PDGFRA Human)
    Tags
    Fc region of IgG1 (a.a. Asp-221 to Lys-447) and L…
    Expression
    Mammalian
    Mutation
    Extracellular domain of PDGFR-alpha (a.a. 1-524)
    Promoter
    CMV
    Available since
    July 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    136438-plasmid-map-sequence-id-271697

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Showing: 10861 - 10880 of 57324 results