We narrowed to 21,642 results for: ESC;

1. AA026

   Plasmid

   #216006

   Purpose

   Fragmid fragment: (Cas protein) NG PAM

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   Cas9-SpG_v1.1 [Sp]
   

   Use

   CRISPR; Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEB2_mScarletI_mVenusNB-MrkHR113A

   Plasmid

   #182292

   Purpose

   expresses c-di-GMP mutant biosensor in E. coli

   Depositor

   Howard Berg

   Article

   Vrabioiu et al Proc Natl Acad Sci U S A. 2022 Feb 8;119

   Insert

   mVenusNB-MrkHR113A
   

   Use

   Tags

   mVenusNB internal fusion to MrkH with a R113A poi…

   Expression

   Bacterial

   Mutation

   changed arginine 113 to alanine, MrkH numbering …

   Promoter

   proC

   Available since

   March 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. Diacylglycerol Kinase Zeta

   Plasmid

   #85454

   Purpose

   expression of Diacylglycerol Kinase Zeta

   Depositor

   Stephen Gee

   Article

   Hogan et al J Biol Chem. 2001 Jul 13;276(28):26526-3

   Insert

   Diacylglycerol Kinase Zeta (DGKZ Human)
   

   Use

   Tags

   3x HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCW17 - pAdapter

   Plasmid

   #174664

   Purpose

   Constitutive expression of λCI fused via three alanines to the MS2 coat protein. Useful as RNA-DNA adapter in three-hybrid system.

   Depositor

   Katherine Berry

   Article

   Pandey et al Nucleic Acids Res. 2020 May 7;48(8):4507

   Insert

   MS2 coat protein
   

   Use

   Tags

   λCI

   Expression

   Bacterial

   Mutation

   V30I, A81G, positions 68-80 deleted (VATQTVGGVELP…

   Promoter

   Synthetic Constitutive Promoter

   Available since

   May 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. hP2X3-cALFA

   Plasmid

   #160499

   Purpose

   For the expression of human P2X3 with ALFA tag at C-terminus in mammalian cells

   Depositor

   Motoyuki Hattori

   Article

   Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

   Insert

   human P2X3 (P2RX3 Synthetic, Human)
   

   Use

   Tags

   HRV3C protease cleavage site-ALFA tag-twin Strep …

   Expression

   Mammalian

   Mutation

   ΔN5ΔC33-T13P/S15V/V16I

   Promoter

   Available since

   Jan. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEX-GFP-hLC3ΔG

   Plasmid

   #24988

   Depositor

   Isei Tanida

   Article

   Tanida et al Autophagy. 2008 Jan 1. 4(1):131-4.

   Insert

   a mutant human MAP1-LC3B fused with EGFP (MAP1LC3B Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   deleted Gly at 121

   Promoter

   Available since

   June 28, 2010

   Availability

   Academic Institutions and Nonprofits only

7. pJH4169

   Plasmid

   #200108

   Purpose

   Prig-3 FRT let858 (stop) FRT snb-1::GFP unc-54 3' UTR C.elegans AVA and other neurons expression of snb GFP

   Depositor

   Mei Zhen

   Article

   Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

   Insert

   snb-1 (snb-1 Nematode)
   

   Use

   Tags

   GFP

   Expression

   Worm

   Mutation

   Promoter

   Prig-3

   Available since

   May 7, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. mCherry Codon 59 sgRNA

   Plasmid

   #109431

   Purpose

   MLM3636 backbone containing a gRNA that guides Cas9 to codon 59 of mCherry in the ACE reporter

   Depositor

   Reuben Harris

   Article

   St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

   Insert

   mCherry codon 59 gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET30b jGCaMP8f F366H

   Plasmid

   #204139

   Purpose

   Production of recombinant calcium sensor jGCaMP8f F366H high dynamic range variant in E.coli

   Depositor

   Katalin Torok

   Article

   Tran et al Front Cell Neurosci. 2023 May 18;17:1155

   Insert

   jGCaMP8f F366H
   

   Use

   Tags

   N-terminal His tag

   Expression

   Bacterial

   Mutation

   Phenylalanine 366 changed to histidine

   Promoter

   T7

   Available since

   Dec. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLENTI_RPS2

    Plasmid

    #127127

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

    Insert

    RPS2 (RPS2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. mCerulean3-Caveolin-C-10

    Plasmid

    #55404

    Purpose

    Localization: Membrane, Excitation: 433, Emission: 475

    Depositor

    Michael Davidson

    Article

    Markwardt et al PLoS One. 2011 . 6(3):e17896.

    Insert

    Caveolin (CAV1 Human)
    

    Use

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pAAV CaMKIIa iChloC 2A tDimer

    Plasmid

    #66709

    Purpose

    Chloride-conducting Channelrhodopsin with neuron-specific promoter, plus red fluorescent protein

    Depositor

    Thomas Oertner

    Article

    Wietek et al Sci Rep. 2015 Oct 7;5:14807. doi: 10.103

    Inserts

    Channelrhodopsin-2
    red fluorescent protein
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    E83Q,E90R,E101S,D156N,T159C

    Promoter

    CaMKIIa

    Available since

    Oct. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMX-hTAF11

    Plasmid

    #44341

    Depositor

    Marc Timmers

    Article

    Pijnappel et al Nature. 2013 Mar 28;495(7442):516-9. doi

    Insert

    TAF11 (TAF11 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    Oct. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMX_hTAF5

    Plasmid

    #44336

    Depositor

    Marc Timmers

    Article

    Pijnappel et al Nature. 2013 Mar 28;495(7442):516-9. doi

    Insert

    TAF5 (TAF5 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    Oct. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEX-HcRed-hLC3WT

    Plasmid

    #24991

    Depositor

    Isei Tanida

    Article

    Tanida et al Autophagy. 2008 Jan 1. 4(1):131-4.

    Insert

    a wild type human MAP1-LC3B fused with HcRed (MAP1LC3B Human)
    

    Use

    Tags

    HcRed

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2010

    Availability

    Academic Institutions and Nonprofits only

16. pK1E-RNAP

    Plasmid

    #48144

    Purpose

    Shuttle vector E. coli/B.meg.; encoding the RNA polymerase of the phage K1E under control of the xylose inducible promoter PxylA

    Depositor

    Dieter Jahn

    Article

    Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

    Insert

    rna polymerase K1E
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. mApple-M1 SpastinK388R

    Plasmid

    #134463

    Purpose

    M1 SpastinK388R expression

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Chang et al J Cell Biol. 2019 Aug 5;218(8):2583-2599

    Insert

    M1 Spastin (SPAST Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Lys to Arg at amino acid 388

    Promoter

    CMV

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCS2+ Zf cdh1-deltaCyot-GFP

    Plasmid

    #185412

    Purpose

    GFP tagged zebrafish cdh1 lacking the cytoplasmic domain.

    Depositor

    Nicolas David

    Article

    Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

    Insert

    cdh1 (cdh1 Zebrafish)
    

    Use

    In vitro synthesis of mrna

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Cytoplasmic domain deleted

    Promoter

    Available since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pICH67131

    Plasmid

    #153223

    Purpose

    Level 1 position 1 containing the Nos promoter, NPTII (Kan resistance), and the Nos terminator

    Depositor

    Sylvestre Marillonnet

    Article

    Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

    Insert

    Nos promoter, NPTII, Nos terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDY118A

    Plasmid

    #182958

    Purpose

    pSC101 ori, chl34 resistant. Cas9 induced at 0.4 μg/ml anhydrotetracycline, recombineering proteins induced by a 15 min heat-shock at 42°C, I-Scel (induced by 0.1 mM IPTG) is to cleave the pDonor2.

    Depositor

    Shelley Copley

    Article

    Yang et al Proc Natl Acad Sci U S A. 2024 Jan 30;12

    Inserts

    Cas9
    Gam, Beta, Exo
    I-scel
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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