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pLKO.1-Tet-Puro-shMMERVK9c_2

Plasmid

#185023

Purpose

shRNA mediated knockdown

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

ERV_MMERVK9c

Use

Lentiviral

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1-Tet-Puro-shMMERVK9c_1

Plasmid

#185022

Purpose

shRNA mediated knockdown

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

ERV_MMERVK9c

Use

Lentiviral

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1-Tet-Puro-shMMERVK10c_2

Plasmid

#185021

Purpose

shRNA mediated knockdown

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

ERV_MMERVK10c

Use

Lentiviral

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1-Tet-Puro-shMMERVK10c_1

Plasmid

#185020

Purpose

shRNA mediated knockdown

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

ERV_MMERVK10c

Use

Lentiviral

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1-Tet-Puro-shMMETn_2

Plasmid

#185019

Purpose

shRNA mediated knockdown

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

ERV_MMETn

Use

Lentiviral

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1-Tet-Puro-shMMETn_1

Plasmid

#185018

Purpose

shRNA mediated knockdown

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

ERV_MMETn

Use

Lentiviral

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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MSCV-Arkadia C946S H948L

Plasmid

#180373

Purpose

expressing mouse Arkadia (isoform 3) C946S H948L E3 ligase dead mutant proteins

Depositor

Dan Littman

Article

Xu et al J Exp Med. 2021 Nov 1;218(11). pii: 2126

Insert

Arkadia (Rnf111 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

C946S H948L

Promoter

LTR promoter

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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mCh-2xFKBP (no insert) (pBS1139)

Plasmid

#185322

Purpose

The empty vector used for the FKBP experiments in our paper

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Type

Empty backbone

Expression

Mammalian

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only
empty-mCh-SspB (pBS1140)

Plasmid

#185323

Purpose

The empty vector used for the iLID-SspB experiments in our paper

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Type

Empty backbone

Expression

Mammalian

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pST_MAHS_RAMVA_mts_1-81 (pBS0918)

Plasmid

#185287

Purpose

For the mammalian expression of the tardigrade protein MAHS_RAMVA_mts_1-81. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

MAHS_RAMVA_mts_1-81

Expression

Mammalian

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pST_MAHS_RAMVA_mts_1-74 (pBS0917)

Plasmid

#185286

Purpose

For the mammalian expression of the tardigrade protein MAHS_RAMVA_mts_1-74. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

MAHS_RAMVA_mts_1-74

Expression

Mammalian

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pST_AfrLEA2 (pBS0910)

Plasmid

#185284

Purpose

For the mammalian expression of the brine shrimp protein AfrLEA2. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

AfrLEA2

Expression

Mammalian

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pST_H2FLK5_CAEEL_30-800 (pBS0864)

Plasmid

#185283

Purpose

For the mammalian expression of the synthetic protein H2FLK5_CAEEL_30-800. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

H2FLK5_CAEEL_30-800

Expression

Mammalian

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pST_APOE_HUMAN_K164E (pBS0843)

Plasmid

#185277

Purpose

For the mammalian expression of the human protein APOE_HUMAN_K164E. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

APOE_HUMAN_K164E

Expression

Mammalian

Mutation

K164E

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pST_APOE_HUMAN_R163C (pBS0842)

Plasmid

#185276

Purpose

For the mammalian expression of the human protein APOE_HUMAN_R163C. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

APOE_HUMAN_R163C

Expression

Mammalian

Mutation

R163C

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pST_APOE_HUMAN_D125I (pBS0840)

Plasmid

#185275

Purpose

For the mammalian expression of the human protein APOE_HUMAN_D125I. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

APOE_HUMAN_D125I

Expression

Mammalian

Mutation

D125I

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pST_APOE_HUMAN_E114C (pBS0838)

Plasmid

#185273

Purpose

For the mammalian expression of the human protein APOE_HUMAN_E114C. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

APOE_HUMAN_E114C

Expression

Mammalian

Mutation

E114C

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pST_APOE_HUMAN_Y136C (pBS0836)

Plasmid

#185272

Purpose

For the mammalian expression of the human protein APOE_HUMAN_Y136C. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

APOE_HUMAN_Y136C

Expression

Mammalian

Mutation

Y136C

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pST_APOE_HUMAN_R160W (pBS0835)

Plasmid

#185271

Purpose

For the mammalian expression of the human protein APOE_HUMAN_R160W. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

APOE_HUMAN_R160W

Expression

Mammalian

Mutation

R160W

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pST_APOE_HUMAN_V153N (pBS0824)

Plasmid

#185270

Purpose

For the mammalian expression of the human protein APOE_HUMAN_V153N. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

APOE_HUMAN_V153N

Expression

Mammalian

Mutation

V153N

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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