We narrowed to 10,985 results for: cat.1
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Plasmid#163672PurposeExpresses a TFR and ST6GAL1 swapped chimera with a truncated cytosolic tail from TFR, TMD from TFR and luminal domain from ST6GAL1 in mammalian cellsDepositorInsertTagsGFPExpressionMammalianMutationThis chimera contains the 9 amino acids from the …Available SinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only
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pCDH_CMV_HMGB_ctail_mKate2_CLEAVAGE
Plasmid#167338PurposeExpresses mKate2 fused to TFAM HMGB+C-tail mutant in mammalian cellsDepositorInsertTFAM (TFAM Human)
UseLentiviralTagsmKate2ExpressionMammalianMutationTruncation mutant containing MTS (1-42) as well a…PromoterCMVAvailable SinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
CPH3568 (YCp LEU2 Rpb1 P1455_E1456InsGGGGGLEVLFQGP(H)10,N1501K; msLink1)
Plasmid#91819PurposeYeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site and 10xHis tag for purification of the linker-CTDDepositorInsertRPO21 (RPO21 Budding Yeast)
Tags10xHisExpressionYeastMutation5xGly linker, PreScission Protease site (LEVLFQGP…PromoterEndogenous Rpb1 promoterAvailable SinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
MAP3K1 gRNA (BRDN0001149426)
Plasmid#77824Purpose3rd generation lentiviral gRNA plasmid targeting human MAP3K1DepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
ACVRL1 gRNA (BRDN0001149122)
Plasmid#77782Purpose3rd generation lentiviral gRNA plasmid targeting human ACVRL1DepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
EPHA5 gRNA (BRDN0001146414)
Plasmid#76676Purpose3rd generation lentiviral gRNA plasmid targeting human EPHA5DepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
DAPK2 gRNA (BRDN0001145554)
Plasmid#76019Purpose3rd generation lentiviral gRNA plasmid targeting human DAPK2DepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
ACVRL1 gRNA (BRDN0001147340)
Plasmid#77783Purpose3rd generation lentiviral gRNA plasmid targeting human ACVRL1DepositorAvailable SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
Ezh2[SET]-dCas9
Plasmid#100087PurposeCatalytic domain [SET] of mouse Ezh2 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expressionDepositorInsertEzh2[SET] (Ezh2 Mouse)
UseCRISPRTags3XFLag-NLS-Ezh2[SET]-dCas9-NLSExpressionMammalianMutationEzh2[SET] includes aa 482-746PromoterCMVAvailable SinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
Beta-2-adrenergic receptor-mRFP
Plasmid#67145PurposeBeta-2-adrenergic receptor tagged with mRFP on carboxyl terminusDepositorInsertBeta-2-adrenergic receptor-mRFP (ADRB2 Discosoma sp., Human)
TagsmRFPExpressionMammalianPromoterCMVAvailable SinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
Beta-2-adrenergic receptor-YFP
Plasmid#67146PurposeBeta-2-adrenergic receptor tagged with YFP on carboxyl terminusDepositorInsertBeta-2-adrenergic receptor-YFP (ADRB2 Human, Aequorea victoria)
TagsYFPExpressionMammalianPromoterCMVAvailable SinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
Beta-2-adrenergic receptor-CFP
Plasmid#55794PurposeBeta-2-adrenergic receptor tagged with ECFP on carboxyl terminusDepositorInsertBeta-2-adrenergic receptor-ECFP (ADRB2 Human, Aequorea victoria)
TagsECFPExpressionMammalianPromoterCMVAvailable SinceAug. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
G protein alpha-s(alpha3beta5/G226A/A366S)
Plasmid#55797PurposeThis highly effective dominant negative G protein alpha-s mutant contains, in addition to the mutations in alpha-s(alpha3beta5/G226A), the A366S mutation, which increases GDP release.DepositorInsertalpha-s (alpha3beta5/G226A/A366S) (Gnas Rat)
Tagsinternal EE epitope (residues 189-194 in alpha-s …ExpressionMammalianMutationN271K, K274D, R280K, T284D, I285T, G226A, A366S i…PromoterCMVAvailable SinceAug. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKan cpRBP-FL P53
Plasmid#174504PurposeExpress Full-length P53 in E.coli with cleavable expression/purification tag (ribose binding protein circular permutant)DepositorInsertP53 gene (TP53 Human)
Tags20 amino acid linker containing HRV3C protease si…ExpressionBacterialPromoterT7Available SinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
Beta-2-adrenergic receptor-mCerulean
Plasmid#66957PurposeBeta-2-adrenergic receptor tagged with mCerulean on carboxyl terminusDepositorInsertBeta-2-adrenergic receptor-mCerulean (ADRB2 Human, Aequorea victoria)
TagsmCeruleanExpressionMammalianPromoterCMVAvailable SinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAV-CMV-scFVtet1_bGHpA
Plasmid#177351PurposeAAV expression of scFV-fused catalytic domain of TET1 for targeted DNA methylation editingDepositorInsertTet Methylcytosine Dioxygenase 1 (TET1 Human)
UseAAVTagsmyc and scFVExpressionMammalianMutationN terminal domain of human Tet1PromoterCMV promoterAvailable SinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-TRE-scFVtet1_bGHpA
Plasmid#177357PurposeAAV expression of scFV-fused catalytic domain of TET1 from doxycycine-inducible promoter for targeted DNA methylation editingDepositorInsertTet Methylcytosine Dioxygenase 1 (TET1 Human)
UseAAVTagsmyc and scFVExpressionMammalianMutationN terminal domain of human Tet1PromoterTetracycline-dependent promoterAvailable SinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
CPH3569 (YCp LEU2 Rpb1 P1455_E1456InsGGGGGLEVLFQGP(H)10,K1487N,D1497K; msLink2)
Plasmid#91820PurposeYeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site and 10xHis tag for purification of the linker-CTDDepositorInsertRPO21 (RPO21 Budding Yeast)
Tags10xHisExpressionYeastMutation5xGly linker, PreScission Protease site (LEVLFQGP…PromoterEndogenous Rpb1 promoterAvailable SinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
T7-VEE-OKS-iG
Plasmid#58974PurposeRNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2 separated by 2A peptides followed by an IRES then GLIS1 and a second IRES and PuroR ORFDepositorUseVenezuelan equine encephalitis (vee) virus rna re…ExpressionMammalianAvailable SinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
SP6-VEE-OKS-iG
Plasmid#58975PurposeRNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2 separated by 2A peptides followed by an IRES then GLIS1 and a second IRES and PuroR ORFDepositorUseVenezuelan equine encephalitis (vee) virus rna re…ExpressionMammalianAvailable SinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only