164,826 results

1. pEGFP-parkin W403A

   Plasmid

   #45876

   Depositor

   Edward Fon

   Article

   Trempe et al Science. 2013 May 9.

   Insert

   Parkin (PRKN Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   W403A mutation

   Promoter

   CMV

   Available Since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

2. pHAGE-CMV-Flag-TLK2_IDG-K

   Plasmid

   #135278

   Purpose

   Gateway destination clone of TLK2 (human) tagged with N-terminal Flag for generating protein-protein networks using fusion tag affinity-based proteomics

   Depositor

   Ben Major

   Insert

   Flag-TLK2 (TLK2 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-Bxb1-His

   Plasmid

   #123132

   Purpose

   Bacterial expression plasmid for wild-type Bxb1 with a C-terminal His-tag.

   Depositor

   David Liu

   Article

   Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

   Insert

   Bxb1 integrase
   

   Use

   Tags

   6x His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   April 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV.hSyn.iGluSnFR3.v857.SGZ

   Plasmid

   #178330

   Purpose

   Fluorescent reporter for glutamate, third generation, variant 857 in Stargazin (SGZ) backbone. iGluSnFR3.v857.SGZ

   Depositor

   Kaspar Podgorski

   Article

   Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

   Insert

   pAAV hSyn iGluSnFR3 v857.SGZ
   

   Use

   AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

   Tags

   IgK-chain, Myc epi tag, and Stargazin and PDZ dom…

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOTTC476 - pAAV c-fos eYFP

   Plasmid

   #47907

   Purpose

   An AAV vector that expresses eYFP under the c-fos promoter.

   Depositor

   Brandon Harvey

   Article

   Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

   Insert

   enhanced Yellow Fluorescent Protein
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   c-fos

   Available Since

   Nov. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. PB-TRE-GNL

   Plasmid

   #204499

   Purpose

   Expresses Glis1, Nanog and lin28a by tet-on promoter in mammalian cells

   Depositor

   Wataru Fujii

   Article

   Matsuya et al Sci Rep. 2024 Feb 8;14(1):3204. doi: 10.

   Insert

   Glis1 (Glis1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pORANGE GFP-Grin2b KI

   Plasmid

   #131487

   Purpose

   Endogenous tagging of GluN2b: N-terminal (amino acid position: S34)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Grin2b Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and Cbh

   Available Since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMPRA2

   Plasmid

   #49350

   Purpose

   Backbone for transient transfection of Massively Parallel Reporter Assays (MPRA) libraries, pPGK-H2Kk for physical selection of transfected cells

   Depositor

   Tarjei Mikkelsen

   Article

   Melnikov et al J Vis Exp. 2014 Aug 17;(90). doi: 10.379

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBABE puro K-Ras V12

   Plasmid

   #9052

   Purpose

   Retroviral expression of a constitutively active form of K-Ras

   Depositor

   William Hahn

   Insert

   KRAS (KRAS Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   G12V. Constitutively active.

   Promoter

   Available Since

   Nov. 14, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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10. eGFP-KIF1A

    Plasmid

    #172206

    Purpose

    Expresses KIF1A tagged with eGFP in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    Guardia et al Cell Rep. 2016 Nov 15;17(8):1950-1961. d

    Insert

    KIF1A (Kif1a Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLL5.0 shCAV1-NLS-mCherry

    Plasmid

    #187257

    Purpose

    Lentiviral expression of shRNA targeting Caveolin1 and NLS-mCherry

    Depositor

    Alpha Yap

    Article

    Katsuno-Kambe et al Biol Cell. 2021 Feb;113(2):107-117. doi:

    Insert

    ShRNA for Caveolin-1 (Cav1 Mouse)
    

    Use

    Lentiviral

    Tags

    NLS-mCherry

    Expression

    Mutation

    Promoter

    U6

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLifeAct_mScarlet_N1

    Plasmid

    #85054

    Purpose

    In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    LifeAct
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDV05.1_pEF1a-iRFP720_frameshifted

    Plasmid

    #200408

    Purpose

    Expresses a mutant iRFP720 mRNA constitutively in mammalian cells. A frameshift deletion in the ORF (compared to pDV05.0) prevents the correct translation of the fluorescent protein

    Depositor

    James Collins

    Article

    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

    Insert

    iRFP720 (frameshift mutant)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. EGFP-Rab5A S34N

    Plasmid

    #28045

    Depositor

    Qing Zhong

    Article

    Sun et al Proc Natl Acad Sci U S A. 2010 Nov 9. 10

    Insert

    Rab5A (RAB5A Human)
    

    Use

    Tags

    EGFP on N-term

    Expression

    Mammalian

    Mutation

    S34N

    Promoter

    Available Since

    Feb. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCFJ782 - HygroR

    Plasmid

    #190933

    Purpose

    Hygromycin resistance co-injection marker

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    Hygromycin resistance gene
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    rps-0

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. FH1-MGT#2-mCherry

    Plasmid

    #164097

    Purpose

    Fluorescent reporter for genetic tracing of mesenchymal Glioblastoma cell-state.

    Depositor

    Gaetano Gargiulo

    Article

    Schmitt et al Cancer Discov. 2021 Mar;11(3):754-777. d

    Insert

    MGT#2-mCherry
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pT3TS nErCas12an

    Plasmid

    #132642

    Purpose

    Used for in vitro transcription of dual NLS ErCas12a mRNA

    Depositor

    Stephen Ekker

    Article

    Wierson et al CRISPR J. 2019 Dec;2(6):417-433. doi: 10

    Insert

    ErCas12a
    

    Use

    CRISPR; In vitro transcription

    Tags

    SV40 NLS

    Expression

    Mutation

    Promoter

    T3

    Available Since

    Nov. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. mAzami-Green-Galectin

    Plasmid

    #102419

    Purpose

    Expresses mAzami-labeled galectin 3 (Gal3) as a marker for endosomal leakage or rupture.

    Depositor

    Geert van den Bogaart

    Article

    Dingjan et al Sci Rep. 2016 Feb 24;6:22064. doi: 10.10

    Insert

    LGALS3 (LGALS3 Human)
    

    Use

    Tags

    mAzami-green

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAVS1-TLR targeting vector

    Plasmid

    #64215

    Purpose

    Targeting vector for the human AAVS1 locus for insertion of traffic light reporter (TLR) construct

    Depositor

    Ralf Kuehn

    Article

    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

    Insert

    traffic light reporter (TLR) (AAVS1 Human)
    

    Use

    Human targeting

    Tags

    Expression

    Mutation

    see publication for details

    Promoter

    CAG

    Available Since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. AP1muA-mStayGold-polyA-Hygro HR

    Plasmid

    #229679

    Purpose

    Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with monomeric StayGold. Contains a hygromycin resistance cassette for selection of edited cells

    Depositor

    Francesca Bottanelli

    Article

    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

    Insert

    genomic sequence AP1M1 locus (AP1M1 Human)
    

    Use

    CRISPR

    Tags

    monomeric StayGold

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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