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pU6_T7_INT[6xminimal_Puf1]

Plasmid

#107608

Purpose

Transient expression of an "INT" construct [bearing six minimal Puf1 stem-loops; 'INT' design: Shechner et al., Nature Methods 2015], in mammalian cells, U6 promoter

Depositor

John Rinn

Article

Maass et al Nat Struct Mol Biol. 2018 Jan 8. pii: 10

Insert

6x minimal Puf1 motif

Use

Synthetic Biology

Expression

Mammalian

Promoter

U6

Available Since

April 2, 2018

Availability

Academic Institutions and Nonprofits only

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pGK:HygroR-CMV:mKO2-C_UTR

Plasmid

#68481

Purpose

Lentivirus expressing mKO2 with UTR sensor cassette and hygromycin resistance

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Inserts

Hygromycin Resistance
mKO2-C_UTR

Use

Lentiviral

Expression

Mammalian

Mutation

shRNA sensor cassette "B" added to 3&#x…

Promoter

CMV and pGK

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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MSCV-miRE_shBRD9_508-SV40-GFP

Plasmid

#75138

Purpose

Retroviral expression plasmid encoding shBRD9_508 (targeting human BRD)

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

shBRD9_508

Use

Retroviral

Available Since

May 23, 2016

Availability

Academic Institutions and Nonprofits only
pCDF1-ERBB4 (E836K)

Plasmid

#29542

Depositor

Yardena Samuels

Article

Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

Insert

ERBB4 (ERBB4 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

July 20, 2011

Availability

Academic Institutions and Nonprofits only
1161F

Plasmid

#183138

Purpose

Plasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 3 gRNA targeting D.suzukii bTub,Hr5Ie1-eGFP tagged, and can be integrated with pBac.

Depositor

Omar Akbari

Article

Kandul et al GEN Biotechnology (2022) 1:4, 372-385

Insert

U6.3-gRNAs[sxl, bTub]

Use

CRISPR

Expression

Insect

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pVMG0302_Cq-U6-1_2xBbsI_gRNA-Loop

Plasmid

#169369

Purpose

Expression of gRNA in Culex quinquefasciatus or other mosquitoes

Depositor

Valentino Gantz

Article

Feng et al Nat Commun. 2021 May 20;12(1):2960. doi:

Insert

pVMG0302_Cq-U6-1_2xBbsI_gRNA-Loop

Use

CRISPR

Expression

Insect

Promoter

CPIJ039653

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pCDF1-ERBB4 (E542K)

Plasmid

#29539

Depositor

Yardena Samuels

Article

Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

Insert

ERBB4 (ERBB4 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only
pAimR

Plasmid

#98687

Purpose

Expresses AimR gene from the B. subtilis phage phi3T, with its native promoter, C' His-tag

Depositor

Rotem Sorek

Article

Erez et al Nature. 2017 Jan 26;541(7638):488-493. d

Insert

AimR

Tags

His-tag

Expression

Bacterial

Promoter

Native

Available Since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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874U=ExuL-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40

Plasmid

#159671

Purpose

Plasmid supports Cas9 expression only in males, Opie2-dsRed tagged, and can be integrated with pBac or phC31.

Depositor

Omar Akbari

Article

Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

Insert

ExuL-NLS-hSpCas9-T2A-GFP

Tags

T2A-GFP

Expression

Insect

Available Since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ERBB4 (R544W)

Plasmid

#29531

Depositor

Yardena Samuels

Article

Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

Insert

ERBB4 (ERBB4 Human)

Expression

Mammalian

Mutation

changed arginine 544 to tryptophan

Available Since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only