164,826 results

1. pRluc-LC3G120A

   Plasmid

   #105003

   Purpose

   Mammalian expression of Rluc-LC3G120A. Can be used for measuring autophagic flux.

   Depositor

   Marja Jããttelã

   Article

   Farkas et al Methods Enzymol. 2017;588:1-13. doi: 10.

   Insert

   LC3B (Map1lc3b Rat)
   

   Use

   Luciferase

   Tags

   Rluc with C124A substitution

   Expression

   Mammalian

   Mutation

   Glycine 120 mutated to alanine, prevents lipidati…

   Promoter

   Available Since

   Jan. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPBbsr2-AREG-ScNeo

   Plasmid

   #209910

   Purpose

   To monitor the status of Amphiregulin, the plasmid encodes a recombinant AREG fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

   Depositor

   Michiyuki Matsuda

   Article

   Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

   Insert

   AREG-ScNeo (AREG Human)
   

   Use

   Tags

   mNeonGreen and mScarlet

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pQTEV-PSMD11

   Plasmid

   #31333

   Depositor

   Konrad Buessow

   Article

   Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

   Insert

   proteasome (prosome, macropain) 26S subunit, non-ATPase, 11 (PSMD11 Human)
   

   Use

   Tags

   His and TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. PB-tetO-NGN2

   Plasmid

   #197090

   Purpose

   PiggyBac plasmid with tet-inducible expression of transcription factors for cortical neuron differentiation

   Depositor

   Brian Wainger

   Article

   Held et al Cell Rep. 2023 Aug 30;42(9):113046. doi:

   Insert

   NGN2 (NEUROG2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRN111

   Plasmid

   #84463

   Purpose

   pJB38-NWMN29-30 + SarA_P1-mCherry-Term

   Depositor

   Reindert Nijland

   Article

   de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

   Insert

   SarA_P1-mCherry from pRN11
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKK-BiFC-Venus

   Plasmid

   #105804

   Purpose

   Concomitant expression of two proteins in fusion with fragments of Venus protein. Protein interactions studies by bimolecular fluorescence complementation (BiFC), including BiFC-FRET assays.

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   VN173 (I152L); VC155

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. SRE reporter vector_559

   Plasmid

   #82686

   Purpose

   Cloning vector containing serum response element (SRE) followed by an AscI restriction site

   Depositor

   Harald Janovjak

   Article

   Reichhart et al Angew Chem Int Ed Engl. 2016 May 17;55(2

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. cytoplasmic EKAR (Cerulean-Venus)

   Plasmid

   #18679

   Depositor

   Karel Svoboda

   Article

   Harvey et al PNAS 2008 Dec 9;105(49):19264-9

   Insert

   ERK Activity Reporter
   

   Use

   Tags

   monomeric Cerulean and monomeric Venus

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 2, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSLQ9757: CMV-BPNLS_TadA-linkerA-TadA*(8e)-Linker B-SV40 NLS-dCasMINI-V4-linkerC-c-Myc_NLS-3x FLAG-polyA

   Plasmid

   #176270

   Purpose

   Expression of TadA-TadA* (8e)-dCasMINI-V4 in mammalian cells

   Depositor

   Stanley Qi

   Article

   Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

   Insert

   TadA-TadA*(8e)-dCasMINI-V4
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRsetB-His7tag-Peredox-mCherry

    Plasmid

    #32382

    Depositor

    Gary Yellen

    Article

    Hung et al Cell Metab. 2011 Oct 5;14(4):545-54.

    Insert

    His7-Peredox-mCherry
    

    Use

    Tags

    His7tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. SETD2-GFP

    Plasmid

    #80653

    Purpose

    mammalian expression of SETD2-GFP

    Depositor

    Sérgio De Almeida

    Article

    Carvalho et al Elife. 2014 May 6;3:e02482. doi: 10.7554

    Insert

    SETD2 (SETD2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSFFV_CloGFP(1-10)

    Plasmid

    #157994

    Purpose

    Lentiviral vector of constitutive expression of CloGFP(1-10)

    Depositor

    Bo Huang

    Article

    Zhou et al PLoS One. 2020 Nov 23;15(11):e0242592. d

    Insert

    CloGFP(1-10)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    G32Q, F46L, K101R, S202L

    Promoter

    SFFV

    Available Since

    Oct. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV6-XL4 FLAG-CBLN4-Fc

    Plasmid

    #115769

    Purpose

    Expression of the extracellular domain of CBLN4 with N-terminal FLAG tag and C-terminal hIgG tag.

    Depositor

    Davide Comoletti

    Insert

    CBLN4 (CBLN4 Human)
    

    Use

    Tags

    FLAG and hIgG/Fc

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    Oct. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMU-TPr

    Plasmid

    #61189

    Purpose

    Fluorescent reporter for tonoplast AtTIP1-1-mCherry expressed under AtUBQ10 promoter

    Depositor

    Maria Harrison

    Article

    Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

    Insert

    AtTIP1-1
    

    Use

    Tags

    mCherry

    Expression

    Plant

    Mutation

    Promoter

    AtUBQ10

    Available Since

    Feb. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV INS2x mRFP-3MREs (AAV-beta)

    Plasmid

    #206928

    Purpose

    Beta cell-specific gene expression from AAV in human pancreatic islets

    Depositor

    Markus Grompe

    Article

    Chai et al Hum Gene Ther. 2022 Aug;33(15-16):789-80

    Insert

    mRFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    INSx2

    Available Since

    Sept. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRS316-OsTIR1F74A-T2A-mAID-Nb (VHHGFP4)

    Plasmid

    #198417

    Purpose

    Expresses both OsTIR1F74A and mAID-Nb(VHHGFP4) under the control of ADH1 promoter in budding yeast

    Depositor

    Takumi Kamura

    Article

    Ogawa et al PLoS Genet. 2023 Jun 14;19(6):e1010731.

    Insert

    OsTIR1F74A-T2A-mAID-Nb (VHHGFP4)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH1 promoter

    Available Since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. Dazl-CPG-TV

    Plasmid

    #70144

    Purpose

    a targeting vector, to target the endogenous DAZL CPG island with Snrp-GFP-polyA

    Depositor

    Rudolf Jaenisch

    Article

    Stelzer et al Cell. 2015 Sep 24;163(1):218-29. doi: 10

    Insert

    5'hDAZL-snrp-GFP-polyA-pgK-PURO-3'hDAZL
    

    Use

    Mouse Targeting and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEYFP-N1-Pink1

    Plasmid

    #101874

    Purpose

    Transient expression of Pink1-YFP in mammalian cells

    Depositor

    Richard Youle

    Article

    Narendra et al PLoS Biol. 2010 Jan 26;8(1):e1000298. do

    Insert

    PTEN induced putative kinase 1 (PINK1 Human)
    

    Use

    Tags

    fluorescent tag EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX330-SpCas9-NG

    Plasmid

    #117919

    Purpose

    Expresses 3xFLAG-NLS-SpCas9-NG-NLS in mammalian cells.

    Depositor

    Osamu Nureki

    Article

    Nishimasu et al Science. 2018 Aug 30. pii: science.aas91

    Insert

    SpCas9-NG (L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. PB-CAG-rtTA-IRES-eGFP-pGK-NeoR

    Plasmid

    #204497

    Purpose

    Expresses rtTA and Neomycin resistance genes in mammalian cells

    Depositor

    Wataru Fujii

    Article

    Matsuya et al Sci Rep. 2024 Feb 8;14(1):3204. doi: 10.

    Insert

    rtTA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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