We narrowed to 18,769 results for: Mut

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relAE335Q_NS1

Plasmid

#100917

Purpose

expresses E.coli relAE335Q from the NS1 site in the S. elongatus PCC7942 genome

Depositor

Erin O'Shea

Article

Puszynska et al Cell Rep. 2017 Dec 12;21(11):3155-3165.

Insert

RelA fragment from E.coli with point mutation E335Q

Use

Cyanobacteria cloning vector

Tags

FLAG

Mutation

E 335 converted to Q

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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pUG-UBC26

Plasmid

#104691

Purpose

Cloning Module

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

AtUBC26 (AT1G53025 Mustard Weed)

Use

Synthetic Biology

Mutation

BpiI/BsaI sites removed by point-mutation

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pCDB325

Plasmid

#113669

Purpose

expresses Ulp1_R1 in Escherichia coli

Depositor

Christopher Bahl

Article

Lau et al J Biol Chem. 2018 Aug 24;293(34):13224-1

Insert

Ulp1_R1

Tags

6-His

Expression

Bacterial

Mutation

contains solubility enhancing mutations

Promoter

T7

Available Since

Sept. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pre-Ubc4 BrG pUC19

Plasmid

#111322

Purpose

for transcription to Ubc4 pre-mRNA

Depositor

Christine Guthrie

Article

Abelson et al Methods Enzymol. 2010;472:31-40. doi: 10

Insert

UBC4 (UBC4 Budding Yeast)

Expression

Bacterial

Mutation

intron has branch-site A to G mutation

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pAW217

Plasmid

#113239

Purpose

E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader ggtaC mutant

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2
Leader-repeat

Expression

Bacterial

Mutation

GGTAG->ggtaC

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pAW215

Plasmid

#113237

Purpose

E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader ggAag mutant

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2
Leader-repeat

Expression

Bacterial

Mutation

GGTAG->ggAag

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAW214

Plasmid

#113236

Purpose

E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader gCtag mutant

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2
Leader-repeat

Expression

Bacterial

Mutation

GGTAG->gCtag

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAW213

Plasmid

#113235

Purpose

E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader Cgtag mutant

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2
Leader-repeat

Expression

Bacterial

Mutation

GGTAG->Cgtag

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAW216

Plasmid

#113238

Purpose

E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader ggtTg mutant

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2
Leader-repeat

Expression

Bacterial

Mutation

GGTAG->ggtTg

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pHBS1333 (IBB-GFP-mCherry 3E splicer)-(BFP-P2A-TDP43 G368W W385G)

Plasmid

#107855

Purpose

To test the effect of sequence on TDP43 splicing activity

Depositor

Rajat Rohatgi

Insert

TARDBP mutant (TARDBP Human)

Expression

Mammalian

Mutation

G368W and W385G in splicing reporter

Available Since

April 13, 2018

Availability

Academic Institutions and Nonprofits only

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pCS2TAL3_DD_NvFP-7R_Ex2-LEFT

Plasmid

#90036

Purpose

TALEN-mediated mutagenesis of Exon 2 in NvFP-7R

Depositor

Matthew Gibson

Article

Ikmi et al Nat Commun. 2014 Nov 24;5:5486. doi: 10.

Insert

7R_Ex2-LEFT

Use

TALEN

Tags

3x Flag and Fok1-DD domain

Promoter

CMV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pCS2TAL3_RR_NvFP-7R_Ex2-RIGHT

Plasmid

#90037

Purpose

TALEN-mediated mutagenesis of Exon 2 in NvFP-7R

Depositor

Matthew Gibson

Article

Ikmi et al Nat Commun. 2014 Nov 24;5:5486. doi: 10.

Insert

7R_Ex2-RIGHT

Use

TALEN

Tags

Fok1-RR domain and HA

Promoter

CMV

Available Since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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pNR166

Plasmid

#79547

Purpose

Output plasmid: contains 5-state register that regulates expression of GFP. Doesn't behave as expected.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

GFPmut3b

Expression

Bacterial

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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pMZ036

Plasmid

#66973

Purpose

Expresses zebrafish Chk1

Depositor

Michael Lampson

Article

Zhang et al Cell Cycle. 2014;13(24):3828-38. doi: 10

Insert

zebrafish Chk1

Use

Zebrafish mrna injection

Expression

Mammalian

Mutation

Q341H mutation compared to GenBank reference sequ…

Promoter

CMV

Available Since

Jan. 13, 2016

Availability

Academic Institutions and Nonprofits only

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c7-87/199_HLL

Plasmid

#63122

Purpose

Mm-cpn cysteine double mutant for crosslinking

Depositor

Tanja Kortemme

Article

Hoersch et al Nat Nanotechnol. 2013 Dec;8(12):928-32.

Insert

Mm-cpn c7_87/199_HLL (NEWENTRY )

Tags

6x HIS-tag

Expression

Bacterial

Mutation

C140S-C237S-C286A-C359T-C393A-C470S-C484S-K87C-S1…

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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c7-132/202_HLL

Plasmid

#63123

Purpose

Mm-cpn double cysteine mutant for crosslinking

Depositor

Tanja Kortemme

Article

Hoersch et al Nat Nanotechnol. 2013 Dec;8(12):928-32.

Insert

Mm-cpn c7_132/202_HLL (NEWENTRY )

Tags

6x HIS-tag

Expression

Bacterial

Mutation

C140S-C237S-C286A-C359T-C393A-C470S-C484S-Q132C-S…

Available Since

April 1, 2015

Availability

Academic Institutions and Nonprofits only

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pFA6a-VN155(I153L)-TRP1

Plasmid

#53193

Purpose

chromosomal tagging with Venus

Depositor

John Pringle

Article

Onishi et al J Cell Biol. 2013 Jul 22;202(2):311-29.

Insert

Venus

Use

Yeast genomic targeting

Tags

TRP1

Mutation

aa 1–155, I to L mutation at aa 153

Available Since

May 7, 2014

Availability

Academic Institutions and Nonprofits only

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pET29b-ClbP-S95A

Plasmid

#48245

Purpose

Untagged, full-length protein with active site mutation Serine95 to Alanine for in vivo peptidase assays

Depositor

Emily Balskus

Article

Brotherton et al J Am Chem Soc. 2013 Mar 6;135(9):3359-62

Insert

ClbP

Expression

Bacterial

Mutation

Changed Serine 95 to Alanine

Promoter

T7

Available Since

March 3, 2014

Availability

Academic Institutions and Nonprofits only

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pICH44233

Plasmid

#50288

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

5'UTR CMV2 (Cucumber Mosaic Virus)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICH44179

Plasmid

#50290

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

5' UTR, RbcS2B (AT5g38420, A. thaliana)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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